The Crop Transformation asnd Genome Editing Unit has a long history of wheat transformation research and has developed optimised protocols for both particle bombardment and Agrobacterium-mediated transformation, successfully transforming over 40 different wheat and wheat-related varieties. Both internal projects and external contracts can be supported with costs being dependent on the service required.
Advice is available on all aspects of transformation from the design stages such as suggesting promoters to drive transgene expression or providing appropriate cloning constructs for either overexpression of genes or their down-regulation by RNAi, to assistance with crossing transgenic plants or taking transgenic lines to field trial.
Recently we have expanded our range of transformed species which now includes rice (Oryza sativa, both indica and japonica cultivars), oilseed rape (Brassica napus) and tomato (Solanum lycopersicum).
We are also at the cutting edge of genome editing, a technology which offers the possibility to generate highly targeted mutations in the host genome. Success has already been achieved in modifying specific target genes in wheat, rice and tomato, and we are delighted to add this method to the range of services offered.
Provided the gene to be introduced is not classed as a significant risk, a simple risk assessment form can be completed to describe the gene and its effects. Otherwise, please provide a full risk assessment by registering a new GM project - please email Mark Wilkinson for more information.