Throughout the last year, field trials of GM and GE crops have continued at Rothamsted despite the various lockdowns and other restrictions that have hampered progress elsewhere.
Rather than ask if genome editing leads to unintended genetic consequences, it’s better to ask if it leads to more changes, and the answer to that is no.
Starting a business can be high risk-high reward, and the ups and downs make it both exciting and challenging. No two days are the same and you never really know what is round the next corner.
CROP TRANSFORMATION & GENOME EDITING - PUBLICATIONS LIST
Raffan, S., Sparks, C. A., Huttly, A. K., Hyde, L., Martignago, D., Mead, A., Hanley, S. J., Wilkinson, P., Barker, G., Edwards, K. J., Curtis, T. Y., Usher, S. L., Kosik, O. and Halford, N. G. 2021. Wheat with greatly reduced accumulation of free asparagine in the grain, produced by CRISPR/Cas9 editing of asparagine synthetase gene TaASN2. Plant Biotechnology Journal. 19 (8), pp. 1602-1613.https://doi.org/10.1111/pbi.13573
Camerlengo, F., Frittelli, A., Sparks, C. A., Doherty, A., Martignago, D., Larre, C., Lupi, R., Sestili, F. and Masci, S. 2020. CRISPR-Cas9 multiplex editing of the α-amylase/trypsin inhibitor genes to reduce allergen proteins in durum wheat. Frontiers in Sustainable Food Systems.https://doi.org/10.3389/fsufs.2020.00104
Hahn, F., Sanjurjo-Loures, L., Sparks, C. A., Kanyuka, K. and Nekrasov, V. 2021. Efficient CRISPR/Cas-Mediated Targeted Mutagenesis in Spring and Winter Wheat Varieties. Plants - Basel. 10 (7), p. 1481.https://doi.org/10.3390/plants10071481
Nekrasov, V., Wang, C., Win, J., Lanz, C., Weigel, D. and Kamoun, S. 2017. Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion. Scientific Reports. 7 (1), p. 482.https://doi.org/10.1038/s41598-017-00578-x
Caroline A. Sparks & Huw D. Jones (2016) “Wheat Biotechnology: Recent Developments and future trends”. In: Biotechnology of Major Cereals. Ed. Huw D. Jones. CABI, Oxfordshire, UK; Boston, MA, USA. Chapter 6, p. 88-103.
TA Bruce, GI Aradottir, LE Smart, JL Martin, JC Caulfield, A Doherty, CA Sparks, CM Woodcock, MA. Birkett, JA Napier, HD Jones and JA Pickett (2015) The first crop plant genetically engineered to release an insect pheromone for defence. Scientific Reports 5, Article number: 11183 doi:10.1038/srep11183.
Caroline A. Sparks and Huw D. Jones (2104) “Genetic transformation of wheat via particle bombardment”. In: Cereal Genomics: Methods and Protocols. Methods in Molecular Biology Ed. Robert J. Henry & Agnelo Furtado. Humana Press, New York, USA. Chapter 17, Vol. 1099, p. 201-218.
Caroline A. Sparks, Angela Doherty & Huw D. Jones (2014) “Genetic transformation of wheat via Agrobacterium-mediated DNA delivery”. In: Cereal Genomics: Methods and Protocols. Methods in Molecular Biology Ed. Robert J. Henry & Agnelo Furtado. Humana Press, New York, USA. Chapter 19, Vol. 1099, p. 235-250.
Freeman, J, Sparks, C. A., West, J., Shewry, P. R., Jones, H. D. (2011) Temporal and spatial control of transgene expression using a heat-inducible promoter in transgenic wheat. Plant Biotechnology Journal 9(7): 788-796.
Huw D. Jones and Caroline Sparks (2008) “Selection of Transformed Plants”. In: Transgenic Wheat, Barley and Oats; Production and Characterisation. Methods in Biotechnology Ed. Jones HD & Shewry PR. Humana Press Totowa, NJ. Chapter 2 p.23-37.
Huw D. Jones and Caroline A. Sparks (2008) “Promoter sequences for defining transgene expression”. In: Transgenic Wheat, Barley and Oats; Production and Characterisation. Methods in Biotechnology Ed. Jones HD & Shewry PR. Humana Press Totowa, NJ. Chapter 11 p.171-184.
Huw D. Jones and Caroline A. Sparks (2008) “Stable Transformation of Plants”. In: Plant Genomics, Methods in Molecular Biology. Eds D Somers, P Langridge and JP Gustafson. Humana Press Totowa, NJ. Chapter 7 p.111-130.
Huw D. Jones, Angela Doherty and Caroline A. Sparks (2008) “Transient Transformation of Plants” In: Plant Genomics, Methods in Molecular Biology. Eds D Somers, P Langridge and JP Gustafson. Humana Press Totowa, NJ. Chapter 8 p.131-152.