Dr ROBERT KING
Computational and Analytical Sciences
Robert is a bioinformatics scientist. He has BSc/MSc/PhD respectively in molecular and cellular biology, applied bioinformatics, and cancer. Robert joined the applied bioinformatics team in October 2013, working on the application of next generation sequencing data to increase yield of agricultural crops, like wheat, and identify molecular targets for pest management. Robert has produced de novo genome assemblies, and corrected new and existing genomes using Illumina and PacBio data, followed by gene annotation/correction using the Maker pipeline with custom Perl/Python scripts. The submission to European Nucleotide Archive and display at Ensembl Fungi of these resources were completed for public access of data. Comparative genomics methods have been used to identify virulence targets using Illumina data by mapping to a reference and counting the read depth to identify presence/absence of regions, using an assembled pangenome. SNP/Indel calling in wheat and associated pathogens including re-assembly of missing wheat gene homologues and differential expression testing have been used to identify targets for crop yield improvement and pathogen reduction. Use and development of protein annotation, secondary metabolite, secretome and effector prediction pipelines have been used to annotate such analyses. Current focus is to use RNA-seq analysis of time course data from fungi and plant species and the integration of omics data sets and associated bioinformatics analyses to identify molecular targets linked to phenotypes.
- 2013 - MSc Applied Bioinformatics, Cranfield University, UK.
- 2007 - Ph.D in Cancer, Queen Mary University of London, UK.
- 2004 - BSc (Hons) in Molecular and Cellular Biology 2:1, University of Kent at Canterbury, UK.