Tailoring Plant Metabolism (ISP) Leader

Plant Sciences


Freddie's research focuses on targeted protein degradation in plants, in particular the N-end rule pathway. A combination of genetics, quantitative proteomics and conventional biochemistry is used to probe the functions and substrates of E3 ligases; the lab is also developing tools to measure protein lifetime in plants. We have a particular interest in protein turnover and oxygen sensing, with a long-term goal of improving flood tolerance in crops. Rothamsted Research is a centre of excellence for plant lipid metabolism, and the lab belongs to the Lipid Signalling and Metabolism "supergroup". We have expertise in peroxisomal transport proteins (especially ABC transporters) involved in lipid catabolism and have also identified novel regulators of lipid breakdown via genetic screens. Our discovery, that targeted protein degradation plays a role in the control of lipid metabolism, unites the two main research themes of the lab.


+44 (0) 1582 938 264


    Rothamsted Research, Harpenden, AL5 2JQ




      • 2000
        Theodoulou, F. L.

        Plant ABC transporters

        Biochimica et Biophysica Acta (BBA) - Biomembranes 1465 : pp.79-103
        doi: 10.1016/S0005-2736(00)00132-2

      • 1998
        Zhou, J-J.,Theodoulou, F. L.,Muldin, I.,Ingemarsson, B.,Miller, A. J.

        Cloning and functional characterization of a Brassica napus transporter that is able to transport nitrate and histidine

        Journal of Biological Chemistry 273 : pp.12017-12023

      • 1997
        Zhou, J-J.,Theodoulou, F. L.,Sauer, N.,Sanders, D.,Miller, A. J.

        A kinetic model with ordered cytoplasmic dissociation for SUC1, an Arabidopsis H+/sucrose cotransporter expressed in Xenopus oocytes

        Journal of Membrane Biology 159 : pp.113-125

      • 1997
        Davies, T. G. E.,Theodoulou, F. L.,Hallahan, D. L.,Forde, B. G.

        Cloning and characterisation of a novel P-glycoprotein homologue from barley

        Gene 199 : pp.195-202

      • 1995
        Theodoulou, F. L.,Miller, A. J.

        Xenopus oocytes as a heterologous expression system for plant proteins

        Molecular Biotechnology 3 : pp.101-115

      • 1994
        Theodoulou, F. L.,Dewey, F. M.,Evans, D. E.

        Calmodulin-stimulated ATPase of maize cells: functional reconstitution, monoclonal antibodies and subcellular localisation

        Journal of Experimental Botany 45 : pp.1553-1564


      Strategic programme

      • Tailoring Plant Metabolism (TPM)

      Science project

      • Cleavage of acyl-CoA by peroxisomal ABC subfamily D transporters in peroximes:mechansim and functional roles

      PhD project

      • Molecular characterisation of the plant N-end rule, a pathway of agricultural importance


      • 1992 - DPhil, Department of Plant Sciences, University of Oxford, UK
      • 1989 - BA (Hons), first class, Botany, University of Oxford, UK


      • 2015 - present - Honorary Professor (Plant Biochemistry), Department of Animal and Plant Sciences, University of Sheffield
      • 2010 -present - Science Editor, The Biochemist, magazine of the Biochemical Society
      • Membership representative, Biochemical Society Awards Committee
      • Steering Committee and founding member, UK Plant Sciences Federation
      • External PhD Examiner for numerous UK and overseas universities