Tailoring Plant Metabolism (ISP) Leader

Plant Sciences


Freddie's research focuses on targeted protein degradation in plants, in particular the N-end rule pathway. A combination of genetics, quantitative proteomics and conventional biochemistry is used to probe the functions and substrates of E3 ligases; the lab is also developing tools to measure protein lifetime in plants. We have a particular interest in protein turnover and oxygen sensing, with a long-term goal of improving flood tolerance in crops. Rothamsted Research is a centre of excellence for plant lipid metabolism, and the lab belongs to the Lipid Signalling and Metabolism "supergroup". We have expertise in peroxisomal transport proteins (especially ABC transporters) involved in lipid catabolism and have also identified novel regulators of lipid breakdown via genetic screens. Our discovery, that targeted protein degradation plays a role in the control of lipid metabolism, unites the two main research themes of the lab.


+44 (0) 1582 938 264


    Rothamsted Research, Harpenden, AL5 2JQ




      • 2015
        Baker, A.,Carrier, D. J.,Schaedler, T. A.,Waterham, H. R.,Van Roermund, C. W.,Theodoulou, F. L.

        Peroxisomal ABC transporters: functions and mechanisms

        : 959-965
        doi: 10.1042/BST20150127 Open Access

      • 2001
        Lao, S-H.,Theodoulou, F. L.,Coleman, J. O. D.,Cole, D.,Edwards, R.

        Role of 3,4-dichloroaniline N -malonyltransferase in the detoxification of herbicides

        In Proceedings Brighton Crop Protection Conference - Weeds 2001 : 349-352

      • 1994
        Evans, D. E.,Theodoulou, F. L.,Williamson, I. M.,Boyce, J. M.

        The calcium pumps of plant cell membranes

        In Membrane transport in plants and fungi: molecular mechanisms and control : 43-59

        Edited by: Leigh, R. A.,Sanders, D.,Blatt, M.

      Strategic programme

      • Tailoring Plant Metabolism (TPM)

      Science project

      • Cleavage of acyl-CoA by peroxisomal ABC subfamily D transporters in peroximes:mechansim and functional roles

      PhD project

      • Molecular characterisation of the plant N-end rule, a pathway of agricultural importance


      • 1992 - DPhil, Department of Plant Sciences, University of Oxford, UK
      • 1989 - BA (Hons), first class, Botany, University of Oxford, UK


      • 2015 - present - Honorary Professor (Plant Biochemistry), Department of Animal and Plant Sciences, University of Sheffield
      • 2010 -present - Science Editor, The Biochemist, magazine of the Biochemical Society
      • Membership representative, Biochemical Society Awards Committee
      • Steering Committee and founding member, UK Plant Sciences Federation
      • External PhD Examiner for numerous UK and overseas universities