Tailoring Plant Metabolism (ISP) Leader

Plant Sciences


Freddie's research focuses on targeted protein degradation in plants, in particular the N-end rule pathway. A combination of genetics, quantitative proteomics and conventional biochemistry is used to probe the functions and substrates of E3 ligases; the lab is also developing tools to measure protein lifetime in plants. We have a particular interest in protein turnover and oxygen sensing, with a long-term goal of improving flood tolerance in crops. Rothamsted Research is a centre of excellence for plant lipid metabolism, and the lab belongs to the Lipid Signalling and Metabolism "supergroup". We have expertise in peroxisomal transport proteins (especially ABC transporters) involved in lipid catabolism and have also identified novel regulators of lipid breakdown via genetic screens. Our discovery, that targeted protein degradation plays a role in the control of lipid metabolism, unites the two main research themes of the lab.


+44 (0) 1582 938 264


    Rothamsted Research, Harpenden, AL5 2JQ




      • 2014
        Kaur, N.,Cross, L.,Theodoulou, F. L.,Baker, A.,Hu, J.

        Plant peroxisomes: protein import, dynamics and metabolite transport

        In Cell biology
        Edited by: Liu, B.,Assmann, S.
        Springer, New York : 25pp
        doi: 10.1007/978-1-4614-7881-2_4-1 ISBN: 9781461478812
      • 2014
        Theodoulou, F. L.,Baldwin, S. A.,Baldwin, J. M.,Baker, A.

        Plant peroxisomal ABC transporters: flexible and unusual

        In Plant ABC transporters. Signalling and Communication in Plants 22
        Edited by: Geisler, M.
        Springer, Cham : 77-101
        doi: 10.1007/978-3-319-06511-3_6 ISBN: 9783319065113
      • 2013
        Linka, N.,Theodoulou, F. L.

        Metabolite transporters of the plant peroxisomal membrane: known and unknown

        In Peroxisomes and their key role in cellular signaling and metabolism
        Edited by: Delrio, L. A.
        Springer, Dordrecht : 169-194
        doi: 10.1007/978-94-007-6889-5_10 ISBN: 9789400768888
      • 2011
        Theodoulou, F. L.,Zhang, X.,De Marcos Lousa, C.,Nyathi, Y.,Baker, A.

        Peroxisomal transport systems: roles in signaling and metabolism

        In Transporters and pumps in plant signaling
        Edited by: Geisler, M.,Venema, K.
        Springer, Berlin : 327-351
        doi: 10.1007/978-3-642-14369-4_12
      • 1995
        Theodoulou, F. L.,Miller, A. J.

        Xenopus oocytes as a heterologous expression system

        In Methods in molecular biology vol. 49: plant gene transfer and expression protocols
        Edited by: Jones, H.
        Humana Press, Totowa : 317-340
      • 1994
        Miller, A. J.,Smith, S. J.,Theodoulou, F. L.

        The heterologous expression of H+-coupled transporters in Xenopus oocytes

        In Membrane transport in plants and fungi: molecular mechanisms and control
        Edited by: Leigh, R. A.,Sanders, D.,Blatt, M. R.
        Company of Biologists, Cambridge : 167-177

      Strategic programme

      • Tailoring Plant Metabolism (TPM)

      Science project

      • Cleavage of acyl-CoA by peroxisomal ABC subfamily D transporters in peroximes:mechansim and functional roles

      PhD project

      • Molecular characterisation of the plant N-end rule, a pathway of agricultural importance


      • 1992 - DPhil, Department of Plant Sciences, University of Oxford, UK
      • 1989 - BA (Hons), first class, Botany, University of Oxford, UK


      • 2015 - present - Honorary Professor (Plant Biochemistry), Department of Animal and Plant Sciences, University of Sheffield
      • 2010 -present - Science Editor, The Biochemist, magazine of the Biochemical Society
      • Membership representative, Biochemical Society Awards Committee
      • Steering Committee and founding member, UK Plant Sciences Federation
      • External PhD Examiner for numerous UK and overseas universities