FACILITY

CEREAL TRANSFORMATION

Rothamsted Research offers a cereal transformation service to generate transgenic wheat plants for research purposes for both internal projects and external contracts.

ABOUT

The Cereal Transformation Group has a long history of wheat transformation research and has developed optimised protocols for both particle bombardment and Agrobacterium-mediated transformation, successfully transforming over 40 different wheat and wheat-related varieties. Both internal projects and external contracts can be supported with costs being dependent on the service required.

Advice is available on all aspects of transformation from the design stages such as suggesting promoters to drive transgene expression or providing appropriate cloning constructs for either overexpression of genes or their down-regulation by RNAi, to assistance with crossing transgenic plants or taking transgenic lines to field trial.

A more recent addition to transformation technology is genome editing which offers the possibility to generate highly targeted mutations in the host genome. Some success has already been achieved in modifying specific target genes and the intention is to add this to the range of services offered.

About the facility

BOOKING THE FACILITY

BOOKING

Please complete the booking form for any transformation work and/or speak to Caroline Sparks to discuss.

DOWNLOAD FORM

COSTINGS

Please contact Caroline Sparks to discuss requirements and costs. For internal projects, transformation is included in the online costing form.

RISK ASSESSMENT

Provided the gene to be introduced is not classed as a significant risk, a simple risk assessment form can be completed to describe the gene and its effects. Otherwise, please provide a full risk assessment by registering a new GM project - please email Caroline Sparks for more information.

GUIDED TOUR

A tour and explanation of the facility is available if required. Please e-mail Caroline Sparks.

CONTACT CEREAL TRANSFORMATION

Cereal Transformation Group
Rothamsted Research
Harpenden
Hertfordshire
AL5 2JQ

Tel: +44 (0)1582 763 133
Email: caroline.sparks@rothamsted.ac.uk