Transformation of fungi with marker genes offers a method for visualising hyphae on roots, nematodes and in soil and observing interactions in situ.  The gene for jellyfish green fluorescent protein (gfp) has been used as a marker in many organisms including fungi.  We have developed a transformation system for Pochonia chlamydosporia and we have observed expression of gfp in regenerated protoplasts (Atkins et al., 2004).

Transformation of nematophagous fungi with gfp

Unfortunately this system is unstable and the gene is lost after a few cycles of cell division: as yet, we have not yet obtained stable transformants that can mainatin the marker gene through several cycles of growth.  (Simon Atkins, Brian Kerry and Penny Hirsch

References:

Atkins S.D., Mauchline, T.H., Kerry, B.R. & Hirsch PR. (2004)  Development of a transformation system for the nematophagous fungus Pochonia chlamydosporia. Mycological Research 108, 654-661.

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