We have compiled a separate Sequences page, which includes accession numbers and the ability to download sequences.
Uptake of host DNA by Plasmodiophora
Bryngelsson et al. (1988) were the first to report that host (brassica) DNA could be detected within Plasmodiophora brassicae. Using Brassica napus and Sinapsis alba tandem repeats as a probe, in in situ hybridization and Southern blotting they showed the presence of host DNA sequences in the resting spores and zoospores of P. brassicae. These host sequences were shown to be incorporated into the parasite during each infection cycle in the form of high molecular weight DNA. The possibility of contamination of the P. brassicae DNA samples with host DNA during preparation was excluded. Crude resting spore preparations were treated with radioactively labelled rape DNA but only negligible amounts bound. Also purified resting spores were treated with DNaseI prior to DNA extraction, but this treatment did not affect the detection of the B. napus-derived tandem repeat.
Buhariwalla et al. (1995) also provided evidence for this. Screening of a random library from DNA extracted from P. brassicae resting spores revealed 2 clones that hybridized to Brassica napus DNA. Sequence analysis demonstrated that these clones were highly homologous to a family of repetitive DNA elements previously cloned from Brassica nigra.
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