Rothamsted Research

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Dr Stephen Thomas

About

Contact

steve.thomas@rothamsted.ac.uk

Rothamsted Research, West Common, Harpenden, Hertfordshire, AL5 2JQ

Roles

  • PD Research Scientist
    PBCS Harpenden

Projects

Past Projects

Project Leader

  • Understanding how DELLA-mediated transcription controls GA-responsive growth and development in Arabidopsis
  • Lawes Trust Studentship: Chemical genetic dissection of GA signalling

Member

  • Enhancing wheat field performance and response to abiotic stress with novel growth-regulatory allelles
  • Understanding the signalling pathways that impact on plant architecture
  • BBSRC Quota Studentship: The role of Gibberellin signalling in reproductive development
  • Investigating the role of gibberellin signalling in the response to drought
  • Industry Funded Studentship: The role of gibberellin signalling in bolting in sugar beet

Profile

Publications

1.  Hedden P and Thomas SG. Gibberellin biosynthesis and its regulation (2012) Biochem. J. 444, 11-25

2. Plackett AR, Powers SJ, Fernandez-Garcia N, Urbanova T, Takebayashi Y, Seo M, Jikumaru Y, Benlloch R, Nilsson O, Ruiz-Rivero O, Phillips AL, Wilson ZA, Thomas SG, Hedden P. Analysis of the Developmental Roles of the Arabidopsis Gibberellin 20-Oxidases Demonstrates That GA20ox1, -2, and -3 Are the Dominant Paralogs. Plant Cell. 2012 Mar 16.

3. Pearce S, Saville R, Vaughan SP, Chandler PM, Wilhelm EP, Sparks CA, Al-Kaff N, Korolev A, Boulton MI, Phillips AL, Hedden P, Nicholson P, Thomas SG. Molecular characterization of Rht-1 dwarfing genes in hexaploid wheat. Plant Physiol. 2011 Dec;157(4):1820-31.

4. Plackett AR, Thomas SG, Wilson ZA, Hedden P. Gibberellin control of stamen development: a fertile field. Trends Plant Sci. 2011 Oct;16(10):568-78.

5. Mutasa-Gottgens, E,  Qi, AM, Mathews, A,  Thomas, S, Phillips, A, Hedden, P (2009) Modification of gibberellin signalling (metabolism & signal transduction) in sugar beet: analysis of potential targets for crop improvement. TRANSGENIC RESEARCH 18, 301-308   

6. Rieu I, Eriksson S, Powers SJ, Gong F, Griffiths J, Woolley L, Benlloch R, Nilsson O, Thomas SG, Hedden P, Phillips AL. “Genetic analysis reveals that C19-GA 2-oxidation is a major gibberellin inactivation pathway in Arabidopsis.Plant Cell. 2008 Sep;20(9):2420-36.

7.  Ivo Rieu, Omar Ruiz-Rivero, Nieves Fernandez-Garcia, Jayne Griffiths, Stephen J. Powers, Fan Gong, Terezie Linhartova, Sven Eriksson, Ove Nilsson, Stephen G. Thomas, Andrew L. Phillips and Peter Hedden.”The gibberellin biosynthetic genes AtGA20ox1 and AtGA20ox2 act, partially redundantly, to promote growth and development throughout the Arabidopsis life cycle.” Plant J. 2008 Feb;53(3):488-504. Epub 2007 Dec 6.

8.  Dijkstra C, Adams E, Bhattacharya A, Page AF, Anthony P, Kourmpetli S, Power JB, Lowe KC, Thomas SG, Hedden P, Phillips AL, Davey MR.”Over-expression of a gibberellin 2-oxidase gene from Phaseolus coccineus L. enhances gibberellin inactivation and induces dwarfism in Solanum species.” Plant Cell Rep. 2008 Mar;27(3):463-70. Epub 2007 Nov 13.

9.  Zentella R, Zhang ZL, Park M, Thomas SG, Endo A, Murase K, Fleet CM, Jikumaru Y, Nambara E, Kamiya Y, Sun TP. “Global analysis of DELLA direct targets in early gibberellin signaling in Arabidopsis.” Plant Cell. 2007 Oct;19(10):3037-57.

10.  Appleford NE, Wilkinson MD, Ma Q, Evans DJ, Stone MC, Pearce SP, Powers SJ, Thomas SG, Jones HD, Phillips AL, Hedden P, Lenton JR. “Decreased shoot stature and grain alpha-amylase activity following ectopic expression of a gibberellin 2-oxidase gene in transgenic wheat.” J Exp Bot. 2007;58(12):3213-26.

11.  Ivo Rieu, Omar Ruiz-Rivero, Nieves Fernandez-Garcia, Jayne Griffiths, Stephen J. Powers, Fan Gong, Terezie Linhartova, Sven Eriksson, Ove Nilsson, Stephen G. Thomas, Andrew L. Phillips and Peter Hedden.”The gibberellin biosynthetic genes AtGA20ox1 and AtGA20ox2 act, partially redundantly, to promote growth and development throughout the Arabidopsis life cycle.” Plant J. 2008 Feb;53(3):488-504. Epub 2007 Dec 6.

12.  Jayne Griffiths, Kohji Murase, Ivo Rieu, Rodolfo Zentella, Zhong-Lin Zhang, Stephen Powers, Fan Gong, Andrew L Phillips, Peter Hedden, Tai-ping Sun and Stephen G Thomas. “Genetic Characterization and Functional Analysis of Gibberellin Receptors in Arabidopsis.” Plant Cell (2006) 18: 3399-3414

13.  Thomas, S.G. and Hedden, P. “Gibberellin metabolism and signal transduction.” Annual Plant Reviews-Plant Hormone Signaling. 2006. 24:147-184.

14.Thomas, S.G., Rieu, I. and Steber, C.M. “Gibberellin metabolism and signalling.” Vitamins and Hormones-Advances in Research and Applications. 2005. 72: 289-338

15.Muangprom, A., Thomas, S.G., Sun, T.-p. and Osborn, T.C. “A novel dwarfing mutation in a ‘Green Revolution’ gene from Brassica rapa.” Plant Physiol. 2005 137(3): 931-938.

16.Thomas, S.G. and Sun, T.-p. “Update on gibberellin signaling. A tale of the tall and the short.” Plant Physiol. (2004) 135: 668-676.

17.Tyler, L., Thomas, S.G., Hu, J., Dill, A., Alonso, J.M., Ecker, J.R. and Sun, T.-p. “DELLA proteins and gibberellin-regulated seed germination and floral development in Arabidopsis.” Plant Physiol. (2004) 135: 1008-1019.

18.Dill, A., Thomas, S.G., Hu, J., Steber, C.M. and Sun, T.-p. “The Arabidopsis F-Box Protein    SLEEPY1 Targets Gibberellin Signaling Repressors for Gibberellin-Induced Degradation.” Plant Cell (2004) 16: 1392-1405.

19.McGinnis, K.M., Thomas, S.G., Soule, J.D., Strader, L.C., Zale, J.M., Sun, T.-p. and Steber C.M. "The Arabidopsis SLEEPY1 gene encodes a putative F-box subunit of an SCF E3 ubiquitin ligase." Plant Cell (2003) 15:1120-1130

20.Curtis, I.S., Ward, D.A., Thomas, S.G., Phillips, A.L., Davey, M.R., Power, J.B., Lowe, K.C., Croker, S.J., Lewis, M.J., Magness, S.L. and Hedden, P. "Induction of dwarfism in transgenic Solanum dulcamara by over- expression of a gibberellin 20-oxidase cDNA from pumpkin." Plant J. (2000) 23:329-338

21.Thomas, S.G., Phillips, A.L. and Hedden, P. "Molecular cloning and functional expression of gibberellin 2-oxidases, multifunctional enzymes involved in gibberellin deactivation." Proc. Natl. Acad. Sci. USA (1999) 96:4698-4703

22.Hedden, P., Phillips, A.L., Coles, J., Thomas, S., Appleford, N., Ward, D., Beale, M. and Lenton, J. "Gibberellin biosynthesis: Genes, regulation and genetic manipulation." RIKEN Review (1999) 21:29-30

23.Hedden, P., Coles, J.P., Phillips, A.L., Thomas, S.G., Ward, D.A., Curtis, I.S., Power, J.B., Lowe, K.C., Davey, M.R., Gray, D., Seymour, G.B. and Thomas, B. "Modification of plant morphology by genetic manipulation of gibberellin biosynthesis." In: Genetic and Environmental Manipulation of Horticultural Crops, K. E. Cockshull (Ed.) (1998) 205-217

24.Henderson, L.M., Thomas, S., Banting, G. and Chappell, J.B. (1997). The arachidonate-activatable, NADPH oxidase-associated H+ channel is contained within the multi-membrane-spanning N-terminal region of gp91-phox. Biochemical Journal 325, 701-705.

25.Soriano, S., Thomas, S., High, S., Griffiths, G., Dsantos, C., Cullen, P. and Banting, G. "Membrane association, localization and topology of rat inositol 1,4,5-trisphosphate 3-kinase B: Implications for membrane traffic and Ca2+ homoeostasis." Biochemical Journal (1997) 324:579-589

26.Thomas, S., Soriano, S., dSantos, C. and Banting, G. "Expression of recombinant rat myo-inositol 1,4,5-trisphosphate 3-kinase B suggests a regulatory role for its N-terminus." Biochemical Journal (1996) 319:713-716

27.Thomas, S., Brake, B., Luzio, J.P., Stanley, K. and Banting, G. (1994). Isolation and sequence of a full-length cDNA encoding a novel rat inositol 1,4,5-trisphosphate 3-kinase. Biochimica et Biophysica Acta 1220, 219-222.

28.Ellis, N.A., Tippett, P., Petty, A., Reid, M., Weller, P.A., Ye, T.Z., German, J., Goodfellow, P.N., Thomas, S. and Banting, G. (1994). PBDX is the XG blood-group gene. Nature Genetics 8, 285-290.

 

 

Qualifications

1996

  • PhD in Biochemistry (Bristol University)

1991

  • BSc in Biochemistry with chemistry (Southampton University)