Rothamsted Research

where knowledge grows

napier

Prof Johnathan Napier

About

Contact

johnathan.napier@rothamsted.ac.uk

+44 (0) 1582 763 133 ext: 2136

Rothamsted Research, West Common, Harpenden, Hertfordshire, AL5 2JQ

Roles

  • Principal Investigator
    BCCP Harpenden

Projects

Past Projects

Principal Investigator

  • Purification and functional characterisation of COMATOSE a peroxisomal ABC transporter from Arabidopsis thaliana
  • Lipid transport and metabolism
  • Genetic Improvement of Algae for Value Added Products (GIAVAP)
  • Molecular characterization and genotype development of transgenic Camelina accumulating high levels of omega-3 long chain polyunsaturated fatty acids

Project Leader

  • Recombinant pharmaceuticals from plants for human health
  • Industrial crops producing added value oils for novel chemicals: ICON
  • Lipid-derived signalling
  • Rational metabolic engineering of oilseed fatty acid composition
  • Elucidating the mechanism of organ size control by KLU-dependent intercellular signalling
  • BBSRC Industry CASE Studentship: Understanding the biosynthesis of omega-3 long chain polyunsaturates in E. huxleyi
  • *fatty acids of the acyl-CoA,tri

Member

  • Towards incorporating the biosynthetic transformation required for Striga inhibition from Desmodium into edible legume intercrops

Publications

  1. Napier JA, Chapman JM, Black M (1989). Calcium-dependent induction of novel proteins by abscisic acid in wheat aleurone tissue of different developmental stages. Planta 179, 156-64 
  2. Larsson KH, Napier JA, Gray JC (1992). Import and processing of the precursor form of the gamma subunit of the chloroplast ATP synthase from tobacco. Plant Mol. Biol. 19, 343-349  
  3. Madueno F, Napier JA, Cejudo FJ, Gray JC (1992). Import and processing of the precursor of the Rieske FeS protein of tobacco chloroplasts. Plant Mol. Biol. 20, 289-299  
  4. Napier JA, Larrson KH, Madueno F, Gray JC (1992). Import and processing of the precursor of the delta subunit of tobacco chloroplast ATP synthase. Plant Mol. Biol. 20, 549-554  
  5. Salter AH, Newman BJ, Napier JA, & Gray JC (1992). Import of the precursor of the chloroplast Rieske iron-sulphur protein by pea chloroplasts. Plant Mol. Biol. 20, 569-574 
  6. Napier JA, Hogland A-S, Plant AL, Gray JC (1992). Chloroplast import of the precursor of the gamma subunit of pea  chloroplast ATP synthase. Plant Mol. Biol. 20: 737-741  
  7. Madueno F, Napier JA, Gray JC (1993). Newly imported Rieske iron-sulphur protein associates with both cpn60 and hsp70 in the chloroplast stroma. Plant Cell 5: 1865-1876. 
  8. Smith MA, Napier JA, Shewry PR, Stymne S, Stobart AK (1994) Expression of a biologically active plant cytochrome b5 in E.coli. Biochem. J. 303: 73-79.                    
  9. Smith MA, Stobart AK, Shewry PR,  Napier JA (1994). Isolation, characterisation and in vitro targeting of a cDNA encoding cytochrome b5 from tobacco.  Plant Mol. Biol. 25: 527-537.   
  10. Shewry PR, Napier JA, Tatham AS (1995). Seed storage proteins: structures and biosynthesis. Plant Cell 7: 945-956. 
  11. Conlan RS, Griffiths LA, Napier JA, Shewry PR, Mantell S, Ainsworth C (1995) Isolation and characterisation of cDNA clones representing the genes encoding the major tuber storage protein (discorin) of yam (Dioscorea cayenensis Lam.) Plant Mol Biol 28: 369-380. 
  12. Napier JA, Smith MA, Stobart AK, Shewry PR (1995) Isolation of a cDNA encoding a cytochrome b5 specifically expressed in developing tobacco seeds. Planta 197: 200-202 
  13. Thoyts PJE, Millichip MI, Stobart AK, Griffiths WT, Shewry PR, Napier JA (1995) Expression and in vitro targeting of a sunflower oleosin. Plant Mol Biol 29: 403-410 
  14. Richard G, Napier JA, Shewry PR (1995) Transport and deposition of cereal prolamins.Plant Physiol Biochem 34: 237-243 
  15. Grimwade B, Tatham AS, Freedman RB, Shewry PR, Napier JA (1996) Comparison of the expression patterns of wheat gluten proteins and proteins involved in the secretory pathway in developing caryopses of wheat. Plant Mol Biol 30: 1067-1073. 
  16. Thoyts PJE, Napier JA, Millichip M, Stobart AK, Griffiths WT, Tatham AS, Shewry PR (1996) Characterisation of a sunflower seed albumin which associates with oil bodies. Plant Science 118: 119-125. 
  17. Napier JA, Stobart AK, Shewry PR (1996) The structure and biogenesis of plant oil bodies: the role of the ER and the oleosin class of proteins: Plant Mol Biol 31: 945-956. 
  18. Sayanova O, Smith MA, Lapinskas P, Stobart AK, Dobson G, Christie WW, Shewry PR, Napier JA (1997) Expression of a borage cDNA containing an N-terminal cytochrome b5 domain results in the accumulation of high levels of?6-desaturated fatty acids in transgenic tobacco. Proc Natl Acad Sci USA 94: 4211-4216. 
  19. Andreeva AV, Evans DE, Hawes CR, Napier JA (1997) Tobacco rab1 cDNA cloning and expression and structure- functional analysis of the Rab1 protein. Russian Journal of Bioorganic Chemistry 23: 164-170. 
  20. Napier JA, Richard G, Turner MFP, Shewry PR (1997) Trafficking of wheat gluten proteins in transgenic tobacco plants: the-gliadin does not contain an ER-retention signal. Planta 203: 488-494.
  21.  Haward SR, Napier JA, Gray JC (1997) Chloroplast SecA functions as a membrane-associated component of the Sec-like translocase of pea chloroplasts. Eur J Biochem 248: 724-730. 
  22. Napier JA, Sayanova O, Stobart AK, Shewry PR (1997) A new class of cytochrome b5 fusion proteins. Biochem J. 328: 717-718 
  23. Napier JA, Hey SJ, Lacey DJ, Shewry PR (1998) Identification of a Caenorhabditis elegans?6-fatty acid -desaturase by heterologous expression in Saccharomyces cerevisiae. Biochem J. 330: 611-614. 
  24. Mazhar H, Qualyle R, Fido RJ, Stobart AK, Napier JA, Shewry PR (1998) Synthesis of storage reserves in developing seeds of sunflower. Phytochemistry 48: 429-432. 
  25. Lacey DJ, Wellner N, Beaudoin F, Napier JA, Shewry PR (1998) Secondary structure of oleosins studied in intact oil bodies isolated from seeds of safflower (Carthamus tinctorius L.) and sunflower (Helianthus annuus L.). Biochem J. 334: 469-477.
  26.  Michaelson LV, Lazarus CM, Griffiths G, Napier JA, Stobart AK (1998) Isolation of a?5 desaturase gene from Mortierella alpina. J. Biol. Chem. 273: 19055-19059.
  27.  Michaelson LV, Napier JA, Lazarus CM, Griffiths G, Stobart AK (1998) Isolation of a?5 desaturase gene from Caenorhabditis elegans. FEBS Letts  439: 215-218. 
  28. Napier JA, Sayanova O, Sperling P, Heinz E (1999) A growing family of cytochrome b5 fusion desaturases.  Trends in Plant Sci. 4: 2-4 
  29. Lacey DJ, Beaudoin F, Dempsey CE, Shewry PR, Napier JA (1999) The accumulation of triacylglycerols within the endoplasmic reticulum of developing seeds of Helianthus annuus. Plant J. 17: 397-406. 
  30. Miflin, B. J., Napier, J. A. & Shewry, P. R. (1999). Improving plant product quality. Nature Biotechnology 17: (Supplement), BV13-BV14. 
  31. Sayanova O, Shewry PR, Napier JA (1999).  Cloning and expression of a fatty acid desaturase from Borago officinalis. J. Ex. Bot 50: 409-410. 
  32. Napier JA, Michaelson LV, Stobart AK (1999)  Plant desaturases: harvesting the fat of the land.  Curr. Op. Plant Biol. 2: 123-127. 
  33. Beaudoin F, Lacey DJ, Napier JA (1999)  The biogenesis of the plant seed oil body: oleosin protein is synthesised by ER-bound ribosomes.  Plant Physiol. Biochem.37: 481-490 
  34. Sayanova O, Napier JA, Shewry PR (1999)   ?6-Unsaturated fatty acids in species and tisses of the Primulaceae.  Phytochemistry 52: 419-422 
  35. Li Z, Chu X, Mouille G, Yan L, Kosar-Hashemi B, Hey S, Napier J, Shewry P, Clarke B, Appels R, Morell M, Rahman S. (1999) The localization and expression of the class II starch synthases in wheat.  Plant Physiol. 120: 1147-1156. 
  36. Sayanova O, Shewry PR, Napier JA. (1999)  Histidine41 of the cytochrome b5 domain of the borage ?6 fatty acid desaturase is essential for enzyme activity.  Plant Physiol. 121: 641-646 
  37. Sayanova O,. Davies GM, Smith MA, Griffiths G, Stobart AK, Shewry PR, Napier JA (1999)  Accumulation of?6-unsaturated fatty acids in transgenic tobacco plants expressing a?6-desaturase from Borago officinalis.  J. Ex. Bot. 50: 1647-1652
  38.  Beaudoin F, Napier JA (2000) The targeting and accumulation of ectopically expressed oleosin in non-seed tissues of Arabidopsis thalianaPlanta 210: 439-445.
  39.  Beaudoin F, Wilkinson BM, Stirling CJ, Napier JA  (2000) In vivo targeting of a sunflower oil body protein in yeast secretory (sec) mutants. Plant J. 23: 159-170.
  40.  Beaudoin F, Michaelson LV, Hey SJ, Lewis MJ, Shewry PR, Sayanova O, Napier JA (2000) Heterologous reconstitution in yeast of the polyunsaturated fatty acid biosynthetic pathway.  Proc. Natl. Acad. Sci. USA 97: 6421-6426.
  41.  Osuna-Castro JA, Rascon-Cruz Q, Napier J, Fido RJ, Shewry PR, Paredes-Lopez O. (2000) Overexpression, purification, and in vitro refolding of the 11S globulin from amaranth seed in Escherichia coli. J Agric Food Chem.;48(11):5249-55.
  42. Birkett MA, Campbell CAM, Chamberlain K, Guerrieri E, Hick AJ, Martin JL, Matthes M, Napier JA, Pettersson J, Pickett JA, Poppy GM, Pow EM, Pye BJ, Smart LE,Wadhams GH, Wadhams LJ, Woodcock CM (2000) New roles for cis-jasmone as an insect semiochemical and in plant defense. Proc. Natl. Acad. Sci. USA 97: 9329-9334.
  43.  Libisch B, Michaelson LV, Lewis MJ, Shewry PR, Napier JA (2000) Chimeras of ?6-fatty acid and ?8-sphingolipid desaturases.  Biochem Biophys. Res. Comm. 279: 779-785.
  44.  Sperling P, Libisch B, ZahringerU, NapierJA, Heinz E (2001) Functional identification of a ?8-sphingolipid desaturase from Borago officinalis.  Arch Biochem. Biophys. 388: 293-298.
  45.  Sayanova O, Beaudoin F, Libisch B, Castel A, Shewry PR, Napier JA. (2001) Mutagenesis and heterologous expression in yeast of a plant ?6-fatty acid desaturase. J Exp Bot.52(360):1581-5.
  46.  Napier JA, Michaelson LV. (2001) Towards the production of pharmaceutical fatty acids in transgenic plants.  J. Sci. Food Agric 81: 883-888.
  47.  Napier JA, Michaelson LV. (2001) Genomic and functional characterization of polyunsaturated fatty acid biosynthesis in Caenorhabditis elegans.   Lipids. 36(8):761-6.
  48.  Lamacchia C, Shewry PR, Di Fonzo N, Forsyth JL, Harris N, Lazzeri PA, Napier JA, Halford NG, Barcelo P. (2001) Endosperm-specific activity of a storage protein gene promoter in transgenic wheat seed.  J Exp Bot. 52(355):243-50.
  49. Napier JA, Beaudoin F, Tatham AS, Alexander LG, Shewry PR (2001) The seed oleosins: structure, properties and biological role.  Advances Botanical Res. 35: 111-138.
  50.  Qi B, Beaudoin F, Fraser T, Stobart AK, Napier JA, Lazarus CM. (2002)  Identification of a cDNA encoding a novel C18-Delta(9) polyunsaturated fatty acid-specific elongating activity from the docosahexaenoic acid (DHA)-producing microalga, Isochrysis galbana. FEBS Lett. 510(3):159-65.
  51.  Napier JA (2002) Plumbing the depths of PUFA biosynthesis: a novel polyketide synthase-like pathway from marine organisms.  Trends Plant Science 7: 51-55
  52.  Lucille G. Alexander, Richard B. Sessions, Anthony R. Clarke, Arthur S. Tatham, Peter R. Shewry, Johnathan A. Napier (2002) Characterization and modelling of the hydrophobic domain of a sunflower oleosin.  Planta 214:546-51.
  53.  Beaudoin F, Gable K, Sayanova O, Dunn T, Napier JA. (2002)A Saccharomyces cerevisiae gene required for heterologous fatty acid elongase activity encodes a microsomal {beta}-keto-reductase. J Biol Chem  277(38):35440-9
  54.  Beaudoin F, Napier JA (2002)  Targeting and memebrane-insertion of a sunflower oleosin in vitro and in Saccharomyces cerevisiae.  Planta 215: 293-303.
  55.  Han G, Gable K, Kohlwein SD, Beaudoin F, Napier JA, Dunn TM.  The Saccharomyces cerevisiae YBR159w gene encodes the 3-ketoreductase of the microsomal fatty acid elongase.  J Biol Chem. 2002 Sep 20;277(38):35440-9.
  56.  Napier JA, Michaelson LV, Dunn TM. (2002) A new class of lipid desaturase central to sphingolipid biosynthesis and signalling. Trends Plant Sci.7: 475-8.
  57.  Michaelson LV, Longman AJ, Sayanova O, Stobart AK, Napier JA. (2002)  Isolation and characterization of a cDNA encoding a Delta8 sphingolipid desaturase from Aquilegia vulgaris. Biochem Soc Trans. 30: 1073-5.
  58.  Napier JA, Michaelson LV, Sayanova O. (2003) The role of cytochrome b5 fusion desaturases in the synthesis of polyunsaturated fatty acids. Prostaglandins Leukot Essent Fatty Acids. 68: 135-43.
  59.  Garton S, Michaelson LV, Beaudoin F, Beale MH, Napier JA.  The dihydroceramide desaturase is not essential for cell viability in Schizosaccharomyces pombe. FEBS Lett. 538: 192-6.
  60.  Zhang Y, Darlington H, Jones HD, Halford NG, Napier JA, Davey MR, Lazzeri PA, Shewry PR. (2003) Expression of the gamma-zein protein of maize in seeds of transgenic barley: effects on grain composition and properties. Theor Appl Genet. 106: 1139-46.
  61.  Spano G, Di Fonzo N, Perrotta C, Platani C, Ronga G, Lawlor DW, Napier JA, Shewry PR. (2003)  Physiological characterization of 'stay green' mutants in durum wheat. J Exp Bot. 54:1415-20.
  62.  Sayanova OV, Beaudoin F, Michaelson LV, Shewry PR, Napier JA.  (2003)  Identification of primula fatty acid delta 6-desaturases with n-3 substrate preferences. FEBS Lett. 542: 100-4.
  63.  Whitney HM, Michaelson LV, Sayanova O, Pickett JA, Napier JA. (2003) Functional characterisation of two cytochrome b(5)-fusion desaturases from Anemone leveillei: the unexpected identification of a fatty acid Delta(6)-desaturase. Planta. 217: 983-92.
  64.  Gable K, Garton S, Napier JA, Dunn TM. (2004)  Functional characterization of the Arabidopsis thaliana orthologue of Tsc13p, the enoyl reductase of the yeast microsomal fatty acid elongating system. J Exp Bot. 55: 543-5.
  65.  Whitney HM, Sayanova O, Pickett JA, Napier JA.  (2004) Isolation and expression pattern of two putative acyl-ACP desaturase cDNAs from Bassia scoparia. J Exp Bot. 55:787-9. 
  66.  Dunn TM, Lynch DV, Michaelson LV, Napier JA. (2004)  A post-genomic approach to understanding sphingolipid metabolism in Arabidopsis thaliana. Ann Bot (Lond). 93: 483-97.
  67.  Tosi P, D'Ovidio R, Napier JA, Bekes F, Shewry PR. (2004)  Expression of epitope-tagged LMW glutenin subunits in the starchy endosperm of transgenic wheat and their incorporation into glutenin polymers. Theor Appl Genet. 108: 468-76.
  68.  Sayanova OV, Napier JA.  (2004) Eicosapentaenoic acid: biosynthetic routes and the potential for synthesis in transgenic plants. Phytochemistry. 65:147-58. 
  69.  Mendoza de Gyves E, Sparks CA, Sayanova O, Lazzari PA, Napier JA, Jones HD (2004) Genetic manipulation of -linolenic acid (GLA) synthesis in a commercial variety of evening primrose (Oenothera sp.).  Plant Biotech. J. 2: 351-357.
  70.  Qi , Fraser T, Mugford S, Dobson G, Sayanova O, Butler J, Napier JA, Stobart AK, Lazarus CM. (2004) Production of very long chain polyunsaturated omega-3 and omega-6 fatty acids in plants.  Nature Biotechnol. 22: 739-45
  71. Napier JA, Beaudoin F, Michaelson LV, Sayanova O (2004) The production of longchain polyunsaturated fatty acids in transgenic plants. Genet Eng (N Y).  26:143-57.
  72. Abbadi A, Domergue F, Bauer J, Napier JA, Welti R, Zähringer U, Cirpus P, Heinz E (2004) Biosynthesis of very-long-chain polyunsaturated fatty acids intransgenic oilseeds: constraints on their accumulation. Plant Cell. 16(10):2734-48. 
  73. Napier JA, Sayanova O, Qi B, Lazarus CM (2004) Progress toward the production of long-chain polyunsaturated fatty acids in transgenic plants. Lipids 39(11):1067-75. 
  74.  Napier JA, Beaudoin F, Michaelson LV, Sayanova O (2004) The production of long chain polyunsaturated fatty acids in transgenic plants by reverse-engineering. Biochimie. 86(11):785-92. 
  75. Borner GH, Sherrier DJ, Weimar T, Michaelson LV, Hawkins ND, Macaskill A, Napier JA, Beale MH, Lilley KS, Dupree P (2005) Analysis of detergent-resistant membranes in Arabidopsis. Evidence for plasma membrane lipid rafts. Plant Physiol. 137(1):104-16. 
  76. Tonon T, Sayanova O, Michaelson LV, Qing R, Harvey D, Larson TR, Li Y, Napier JA, Graham IA (2005) Fatty acid desaturases from the microalga Thalassiosira pseudonana. FEBS J.  272(13):3401-12. 
  77. Napier JA, Sayanova O (2005) The production of very-long-chain PUFA biosynthesis in transgenic plants: towards a sustainable source of fish oils. Proc Nutr Soc. 64(3):387-93. 
  78. Sayanova O, Haslam R, Qi B, Lazarus CM, Napier JA (2006) The alternative pathway C20 D8-desaturase from the non-photosynthetic organism Acanthamoeba castellanii is an atypical cytochrome b5-fusion desaturase. FEBS Lett. 580(8):1946-52. 
  79. Paul S, Gable K, Beaudoin F, Cahoon E, Jaworski J, Napier JA, Dunn TM (2006) Members of the Arabidopsis FAE1-like 3-ketoacyl-CoA synthase gene family substitute for the Elop proteins of Saccharomyces cerevisiae. J Biol Chem. 281(14):9018-29. 
  80. Baker JM, Hawkins ND, Ward JL, Lovegrove A, Napier JA, Shewry PR, Beale MH (2006) A metabolomic study of substantial equivalence of field-grown genetically modified wheat. Plant Biotechnol J. 4(4):381-92. 
  81. Sayanova O, Haslam R, Venegas-Calerón M, Napier JA (2006) Identification of Primula "front-end" desaturases with distinct n-6 or n-3 substrate preferences. Planta. 224(6):1269-77. 
  82. Sayanova O, Haslam R, Guschina I, Lloyd D, Christie WW, Harwood JL, Napier JA (2006)  A bifunctional D12,D15-desaturase from Acanthamoeba castellanii directs the synthesis of highly unusual n-1 series unsaturated fatty acids. J Biol Chem. 281(48):36533-41. 
  83. Napier JA (2007) The production of unusual fatty acids in transgenic plants. Annu Rev Plant Biol. 58:295-319. 
  84. Venegas-Calerón M, Beaudoin F, Sayanova O, Napier JA (2007) Co-transcribed genes for long chain polyunsaturated fatty acid biosynthesis in the protozoon Perkinsus marinus include a plant-like FAE1 3-ketoacyl coenzyme A synthase. J Biol Chem. 282(5):2996-3003.
  85. Capuano F, Beaudoin F, Napier JA, Shewry PR (2007) Properties and exploitation of oleosins. Biotechnol Adv. 25(2):203-6. 
  86. Graham IA, Larson T, Napier JA (2007) Rational metabolic engineering of transgenic plants for biosynthesis of omega-3 polyunsaturates. Curr Opin Biotechnol. 18(2):142-7. 
  87. Lefebvre B, Furt F, Hartmann MA, Michaelson LV, Carde JP, Sargueil-Boiron F, Rossignol M, Napier JA, Cullimore J, Bessoule JJ, Mongrand S (2007) Characterization of lipid rafts from Medicago truncatula root plasma membranes: a proteomic study reveals the presence of a raft-associated redox system. Plant Physiol. 144(1):402-18. 
  88. Sayanova O, Haslam R, Venegas Caleron M, Napier JA (2007) Cloning and characterization of unusual fatty acid desaturases from Anemone leveillei: identification of an acyl-coenzyme A C20 D5-desaturase responsible for the synthesis of sciadonic acid. Plant Physiol. 144(1):455-67. 
  89. García-Maroto F, Garrido-Cárdenas JA, Michaelson LV, Napier JA, Alonso DL (2007) Cloning and molecular characterisation of a D8-sphingolipid-desaturase from Nicotiana tabacum closely related to D6-acyl-desaturases. Plant Mol Biol. 64(3):241-50 
  90. Pickett JA, Birkett MA, Bruce TJ, Chamberlain K, Gordon-Weeks R, Matthes MC, Napier JA, Smart LE, Woodcock CM (2007)  Developments in aspects of ecological phytochemistry: the role of cis-jasmone in inducible defence systems in plants. Phytochemistry. 68(22-24):2937-45. 
  91. Bruce TJ, Matthes MC, Chamberlain K, Woodcock CM, Mohib A, Webster B, Smart LE, Birkett MA, Pickett JA, Napier JA (2008) cis-Jasmone induces Arabidopsis genes that affect the chemical ecology of multitrophic interactions with aphids and their parasitoids. Proc Natl Acad Sci U S A. 105(12):4553-8. 
  92. Bach L, Michaelson LV, Haslam R, Bellec Y, Gissot L, Marion J, Da Costa M, Boutin JP, Miquel M, Tellier F, Domergue F, Markham JE, Beaudoin F, Napier JA, Faure JD (2008) The very-long-chain hydroxy fatty acyl-CoA dehydratase PASTICCINO2 is essential and limiting for plant development. Proc Natl Acad Sci U S A. 105(38):14727-31. 
  93. Matthes MC, Pickett JA, Napier JA (2008) Natural variation in responsiveness of Arabidopsis thaliana to methyl jasmonate is developmentally regulated. Planta.  228(6):1021-8. 
  94. Linka N, Theodoulou FL, Haslam RP, Linka M, Napier JA, Neuhaus HE, Weber AP (2008) Peroxisomal ATP import is essential for seedling development in Arabidopsis thaliana. Plant Cell. 20(12):3241-57. 
  95. Michaelson LV, Zäuner S, Markham JE, Haslam RP, Desikan R, Mugford S, Albrecht S, Warnecke D, Sperling P, Heinz E, Napier JA (2009) Functional characterization of a higher plant sphingolipid D4-desaturase: defining the role of sphingosine and sphingosine-1-phosphate in Arabidopsis. Plant Physiol. 149(1):487-98. 
  96. Reina-Pinto JJ, Voisin D, Kurdyukov S, Faust A, Haslam RP, Michaelson LV, Efremova N, Franke B, Schreiber L, Napier JA, Yephremov A (2009) Misexpression of FATTY ACID ELONGATION1 in the Arabidopsis epidermis induces cell death and suggests a critical role for phospholipase A2 in this process. Plant Cell. 21(4):1252-72. 
  97. Beaudoin F, Wu X, Li F, Haslam RP, Markham JE, Zheng H, Napier JA, Kunst L (2009) Functional characterization of the Arabidopsis beta-ketoacyl-coenzyme A reductase candidates of the fatty acid elongase. Plant Physiol. 150(3):1174-91. 
  98. Ruiz-López N, Haslam RP, Venegas-Calerón M, Larson TR, Graham IA, Napier JA, Sayanova O (2009) The synthesis and accumulation of stearidonic acid in transgenic plants: a novel source of 'heart-healthy' omega-3 fatty acids. Plant Biotechnol J. 7(7):704-16. 
  99. Venegas-Calerón M, Sayanova O, Napier JA (2010) An alternative to fish oils: Metabolic engineering of oil-seed crops to produce omega-3 long chain polyunsaturated fatty acids. Prog Lipid Res. 49(2):108-19 
  100. Napier JA, Haslam RP (2010) As simple as ACB--new insights into the role of acyl-CoA-binding proteins in Arabidopsis. New Phytol. 186(4):781-3. 
  101. Napier JA, Graham IA (2010) Tailoring plant lipid composition: designer oilseeds come of age. Curr Opin Plant Biol. 13(3):330-7. 
  102. Venegas-Calerón M, Beaudoin F, Garcés R, Napier JA, Martínez-Force E (2010) The sunflower plastidial omega3-fatty acid desaturase (HaFAD7) contains the signalling determinants required for targeting to, and retention in, the endoplasmic reticulum membrane in yeast but requires co-expressed ferredoxin for activity. Phytochemistry 71(10):1050-8. 
  103. Matthes MC, Bruce TJ, Ton J, Verrier PJ, Pickett JA, Napier JA (2010) The transcriptome of cis-jasmone-induced resistance in Arabidopsis thaliana and its role in indirect defence. Planta. 232(5):1163-80.
  104. Michaelson LV, Dunn TM, Napier JA. (2010) Viral trans-dominant manipulation of algal sphingolipids. Trends Plant Sci. 15(12):651-5. 
  105. Zadravec D, Tvrdik P, Guillou H, Haslam R, Kobayashi T, Napier JA, Capecchi MR, Jacobsson A (2011) ELOVL2 controls the level of n-6 28:5 and 30:5 fatty acids intestis, a prerequisite for male fertility and sperm maturation in mice. J Lipid Res. 52(2):245-55. 
  106. Tavares S, Grotkjær T, Obsen T, Haslam RP, Napier JA, Gunnarsson N. (2011) Metabolic engineering of Saccharomyces cerevisiae for production of Eicosapentaenoic Acid, using a novel D5-Desaturase from Paramecium tetraurelia. Appl Environ Microbiol. 77(5):1854-61. 
  107. Lu C, Napier JA, Clemente TE, Cahoon EB (2011) New frontiers in oilseed biotechnology: meeting the global demand for vegetable oils for food, feed, biofuel, and industrial applications. Curr Opin Biotechnol. 22(2):252-9. 
  108. Bourdenx B, Bernard A, Domergue F, Pascal S, Léger A, Roby D, Pervent M, Vile D, Haslam RP, Napier JA, Lessire R, Joubès J (2011) Overexpression of Arabidopsis ECERIFERUM1 promotes wax very-long-chain alkane biosynthesis and influences plant response to biotic and abiotic stresses. Plant Physiol. 156(1):29-45. 
  109. Sayanova O, Haslam RP, Calerón MV, López NR, Worthy C, Rooks P, Allen MJ, Napier JA (2011) Identification and functional characterisation of genes encoding the omega-3 polyunsaturated fatty acid biosynthetic pathway from the coccolithophore Emiliania huxleyi. Phytochemistry 72(7):594-600

Qualifications

2006

  • DSc in Plant Biotechnology (University of Nottingham)

1989

  • PhD in Plant Biochemistry (King's College, University of London)

1984

  • BSc in Agricultural Sciences (University of Nottingham)