Rothamsted Research

where knowledge grows

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Jacqueline Freeman

Role(s):
Research Scientist
Plant Sciences
Harpenden
Phone:
+44 (0) 1582 763 133 ext: +44 1582 938537
Location:
Rothamsted Research, West Common, Harpenden, Hertfordshire, AL5 2JQ

Freeman, J., Lovegrove, L., Wilkinson, M.D., Saulnier, L., Shewry, P.R. and Mitchell, R.A.C. (2016). Effect of suppression of arabinoxylan synthetic genes in wheat endosperm on chain length of arabinoxylan and extract viscosity. Plant Biotech J., 14, 109-116.

Shewry, P.R., Reynolds, S., Pellny, T., Freeman, J., Wilkinson, M., Kosik, O., Ulker, M., Wingen, L., Orford, S., Griffiths, S., Mitchell, R.A.C. and Lovegrove, A. (2015) Improving wheat as a source of dietary fibre for human health. Proceedings of the Nutrition Society 74, E87 (doi: 10.1017/S0029665115001020).

Shewry, P.R., Saulnier, L., Gebruers, K., Mitchell, R.A.C., Freeman, J., Nemeth, C and Ward, J.L. (2014). Optimising the content and composition of dietary fibre in wheat grain for end use quality. In: Genomics of Plant Genetic Resources, Volume 2, Crop productivity, food security and nutritional quality, (Tuberosa, R., Graner, A and Frison, E., ed.) p 455-466. Springer, Netherlands.

Lovegrove, A., Wilkinson, M.D., Freeman, J. Pellny, T.K., Tosi, P., Saulnier, L., Shewry, P.R. and Mitchell, R.A.C. (2013) RNA Interference Suppression of Genes in Glycosyl Transferase Families 43 and 47 in Wheat Starchy Endosperm Causes Large Decreases in Arabinoxylan Content. Plant Physiology, 163, 95–107.

Molinari, H.B.C., Pellny, T.K., Freeman, J., Shewry, P.R. and Mitchell, R.A.C. (2013) Grass cell wall feruloylation: distribution of bound ferulate and candidate gene expression in Brachypodium distachyon. Frontiers in Plant Science, 4, article 50. (DOI:10.3389/fpls.2013.00050)

Shewry, P.R., Mitchell, R.A.C.,  Tosi, P., Wan, Y., Underwood, C., Lovegrove, A., Freeman, J., Toole, G.A., Mills, E.N.C. and Ward, J.L. (2012) An integrated study of grain development of wheat (cv Hereward). Journal of Cereal Science, DOI:10.1016/j.jcs.2011.11.007

Pellny, T.K., Lovegrove, A., Freeman, J., Tosi, P., Love, C.G., Knox, J.P., Shewry, P.R. and Mitchell, R.A.C. (2012) Cell Walls of Developing Wheat Starchy Endosperm: Comparison of Composition and RNA-Seq Transcriptome. Plant Physiology, 158, 612-627.

Anders, N., Wilkinson, M., Lovegrove, A., Freeman, J., Tryfona, T., Pellny, T.K., Weimar, T., Baker, J., Shewry, P.R., Dupree, P. and Mitchell, R.A.C. (2012) Glycosyl transferases in family 61 mediate arabinofuranosyl transfer onto xylan in grasses. P.N.A.S., 109, 989-993.

Freeman, J., Sparks, C.A., West, J., Shewry, P.R. and Jones, H.D. (2011) Temporal and spatial control of transgene expression using a heat-inducible promoter in transgenic wheat. Plant Biotech. Journal, 9, 788-796.

Toole, G. A., Le Gall, G., Colquhoun, I. J., Nemeth, C., Saulnier, L., Lovegrove, A., Pellny, T., Wilkinson, M. D., Freeman, J., Mitchell, R. A. C., Mills, E. N. C., Shewry, P. R. (2010) Temporal and spatial changes in cell wall composition in developing grains of wheat cv. Hereward. Planta, 232, 677-689.

Shewry, P.R., Freeman, J., Wilkinson, M., Pellny, T. and Mitchell, R.A.C. (2010). Challenges and opportunities for using wheat for biofuel production. In: Energy Crops, (Halford, H.G. and Karp, A., ed.), p. 13-26. RSC publishing, London.

Nemeth, C., Freeman, J., Jones, H.D., Sparks, C., Pellny, T.K., Wilkinson, M.D., Dunwell, J., Andersson, A.A.M., Åman, P., Guillon, F., Saulnier, L., Mitchell, R.A.C. and Shewry, P.R. (2010) Down-Regulation of the CSLF6 Gene Results in Decreased (1,3;1,4)-β-D-Glucan in Endosperm of Wheat. Plant Physiology, 152, 1209-1218.

Van Herpen, T.W.J.M., Cordewener, J.H.G., Klok, H.J., Freeman, J., Bosch, D., Smulders, M.J.M., Gilissen, L.J.W.J., Shewry, P.R. and Hamer, R.J. (2008) The origin of wheat glutenin particles and early development. J. Cereal Science, 48, 3, 561-880.

Wellner, N., Mills, E.N.C., Brownsey, G., Wilson, R.H., Brown, N., Freeman, J., Halford, N.G., Shewry, P.R. and Belton, P.S. (2005) Changes in protein secondary structure during gluten deformation studied by dynamic fourier transform infrared spectroscopy. Biomacromolecules, 6, 255-261.

Freeman, J., Ward, E., Gutteridge, R.J. and Bateman, G.L. (2005) Methods for studying population structure, including sensitivity to the fungicide silthiofam, of the cereal take-all fungus, Gaeumannomyces graminis var. tritici. Plant Pathology, 54, 686-698.

Freeman, J. and Ward, E. (2004) Gaeumannomyces graminis, the take-all fungus and its relatives. Molecular Plant Pathology 5, 235-252.

Calderon, C., Ward, E., Freeman, J., Foster, S.J. and McCartney, H.A. (2002). Detection of airborne inoculum of Leptosphaeria maculans and Pyrenopeziza brassicae in oilseed rape crops by polymerase chain reaction (PCR) assays. Plant Pathology, 51: 303-310.

Freeman, J., Ward, E., Calderon, C. amd McCartney, H.A. (2002). A polymerase chain reaction (PCR) assay for the detection of inoculum of Sclerotinia sclerotiorum. European Journal of Plant Pathology, 108: 877-886 

Calderon, C., Ward, E., Freeman, J. and McCartney, H.A., 2001. Detection of airborne fungal spores sampled by rotating-arm and Hirst-type spore traps using polymerase chain reaction assays. Journal of Aerosol Science, 33: 283-296.

Freeman J, Marquez AJ, Wallsgrove RM, Saarelainen R and Forde BG.  (1990).  Molecular analysis of barley mutants deficient in chloroplast glutamine synthetase.  Plant Mol Biol, 14, 517-525.

Forde BG, Freeman J, Oliver JE and Pineda M.  (1990).  Nuclear factors interact with conserved A/T-rich elements upstream of a nodule-enhanced glutamine synthetase gene from French bean.  Plant Cell, 2, 925-939.

Kreis M, Williamson MS, Buxton B, Pywell J, Hejgaard J and Svendson I.  (1987).  Primary structure and differential expression of b-amylase in normal and mutant barleys.  Eur J Biochem, 169, 517-525.

Kreis M, Williamson MS, Forde J, Clark J, Buxton B, Pywell J, Marris C, Gallois P and Shewry PR.  (1987).  Structure and regulation of expression of seed protein genes in barley.  Plant Physiol Biochem, 25, 291-302.

Kreis M, Williamson MS, Forde J, Schmutz D, Clark J, Buxton B, Pywell J, Marris C, Henderson J, Harris N, Shewry PR, Forde BG and Miflin BJ.  (1986).  Differential gene expression in the developing barley endosperm.  Phil Trans Roy Soc Lond, 355-365.

Forde BG, Heyworth A, Pywell J and Kreis M.  (1985).  Nucleotide sequence of a B1 hordein gene and the identification of possible upstream regulatory elements in endosperm storage protein genes from barley, wheat and maize.  NAR, 13, 7327-7339.

Forde BG, Kreis M, Williamson MS, Fry RP, Pywell J, Shewry PR, Bunce N and Miflin BJ.  (1985).  Short tandem repeats shared by B- and C-hordein cDNAs suggest a common evolutionary origin for two groups of cereal storage protein genes.  EMBO J, 4,9-15.

Kreis M, Rahman S, Forde BG, Pywell J, Shewry PR and Miflin BJ.  (1983).  Sub-families of hordein mRNA encoded at the Hor2 locus of barley.  Mol Gen Genet, 191, 194-200.

Qualifications

2004:

PhD in Molecular Plant Pathology ()

2000:

MSc in Molecular Biology ()

1987:

BSc in Applied Biology ()

Measures of esteem

2016

  • Improvement of wheat grain residue viscosity for non-food uses by reverse genetics: AAB conference. Wheat: genetic improvement and end-use quality.
  • Xylan in grasses â?? composition and structure of arabinoxylan: 1st LBNet International conference, Shrigley Hall, Cheshire

2015

  • Member of AAB

2014

  • Wheat genes for enhanced dietary fibre to improve human health: AAB conference. Wheat breeding 2014: tools, targets and progress.

2013

  • Methods for measuring cell wall feruloylation: EMBRAPA/BBSRC 1st BrachyBAHD workshop, Brasilia

2012

  • Member of Biosciences Knowledge Transfer Network
  • Induced and natural genetic variation in arabinoxylan content of wheat grain: tailoring properties for different end-uses. 9th Monogram Conference, March, 2012, Aberystwyth, UK.: Authors: Freeman, J, Lovegrove, A, Wilkinson, M, Pellny, T, Ulker, M, Shewry, P.R. and Mitchell, R.A.C.
  • Co-supervisor of BBSRC funded IBTI Training Club PhD student Lucy Hyde

2011

  • Cell wall feruloylation and expression of putative feruloyl transferase genes in Brachypodium distachyon and wheat.: Monogram Network Workshop. Nottingham University, Sutton Bonnington Campus.

2010

  • Investigating the role of BAHD genes in feruloylation of wheat endosperm arabinoxylan: XII Cell Wall meeting, Porto, Portugal

2000

  • Zetatronics prize for the best student on a Biosciences pathway: Awarded by University of Hertfordshire in recognition of grades achieved during MSc degree