The Molecular Biology Notebook Online
A Beginners' Guide to Molecular Biology

---

Restriction enzyme

---

A restriction enzyme cuts DNA at specific sites (restriction sites) which correspond to particular sequences of DNA. Restriction enzymes are used in molecular biology to analyse DNA.

View Dr Chromo's lecture on 'Restriction digest'.

---

Pronounce:

---

Related Links

This is a search for restriction in our database

• BVtech Download and description: BVTech Plasmid is DNA plasmid drawing software.Ii is free software.You can use it to draw circle or linear plasmid map with double strands or single strand. You can label the plasmid with genes and restriction sites in different colors, text, and styles.
• MultiCut: restriction enzyme buffer search: Multi-cut is a database of restriction endonuclease buffers. It finds compatible buffers for a list of enzymes that you want to use in a multiple restriction endonuclease digest. Multi-Cut searches through activity data from the catalogs of several major restriction endonuclease manufacturers and finds buffers that will work with all of the endonucleases in the reaction.
• pDRAW32 DNA analysis software : Draw plasmid, restriction analysis..
• RESearch - a new computer program for DNA analysis: The main design philosophy behind the development of RESearch was to make the basic DNA analysis tools readily available on IBM PCs, and to make them easy to use. RESearch enables molecular biologists to analyse a GCG formatted sequence in several ways including searching for short nucleotide sequences and restriction enzyme sites, translating open reading frames and searching for short amino acid sequences.
• Restriction Mapper: Restriction Mapper is a web site that finds restriction endonuclease cleavage sites in DNA sequences. It supports linear and circular DNA and provides several ways to sort and filter output.
• SeqSearch: SeqSearch is build around a collection of databases for restriction enzymes, DNA sequences, plasmids, contructs, oligo's and motifs and offers an extended set of editing and analysis tools.
• UGENE - Unipro: UGENE is a free cross-platform genome analysis suite. Multiple sequence alignment using MUSCLE 3,4 and KAlign; HMM profiles build and search, based on the source of HMMER 2 and HMMER 3; PCR Primers design using Primer 3; Protein secondary structure prediction using GOR IV and PSIPRED; Phylogenetic analysis with Phylip; Search for restriction enzymes and integration with REBASE; Extremely fast repeat finder; DNA reference assembly using Bowtie; Search for transcription factor binding sites using SITECON; Protein back translation; ORF finder; Complete Smith-Waterman algorithm implementation; Comparing genomes using dotplot view.
• Vector Data from Invitrogen: Information on Invitrogen Vector Data Files: The search will display a table containing links to Vector Maps, DNA Sequences (ASCII/Text format), Restriction analyses (ASCII/Text format) and multiple cloning site detail sheets (pdf).
• WebCutter: Webcutter is an on-line tool for restriction mapping nucleotide sequences. Now the new version Cutter"

restriction on Wikipedia

restriction at Answers. com

---

Copyright Rothamsted Research 1999- 2010
Web technical : Nathalie Castells-Brooke

---