The Molecular Biology Notebook Online
Phosphoric acid
Phosphoric acid is an important component of DNA, RNA, and other related compounds, eg. ATP. Highly reactive acid giving rise to phosphates.
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- DNA ligation protocol: DNA ligation is the process of joining together two DNA molecule ends (either from the same or different molecules). Specifically, it involves creating a phosphodiester bond bond between the 3\' hydroxyl of one nucleotide and the 5\' phosphate of another. This reaction is usually catalyzed by a DNA ligase enzyme. This enzyme will ligate DNA fragments having blunt or overhanging, complementary, \'sticky\' ends. Typically, it is easier to ligate molecules with complementary sticky ends than blunt ends. T4 DNA ligase is the most commonly used DNA ligase for molecular biology techniques and can ligate \'sticky\' or blunt ends.
phosphate on Wikipedia