The Molecular Biology Notebook Online
Nested PCR
A nested PCR is the second round of amplification on a fragment which is already the result of a PCR reaction.
In the "2nd" round reaction, the set of primers will be internal to those of the first round, thus amplifying a slightly shorter fragment.
Dr Chromo's lecture on 'PCR'.
Dr Chromo's lecture on 'Sequencing'.
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