Animated Dictionnary Definition

Blot, blotting

To blot is usually to mop up liquid with an absorbant material. It is also a technique used to transfer DNA from an agarose gel to a nylon membrane.

View Dr Chromo's lecture on transfer and hybridization

Pronounce:

Pronounce: blotting

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This is a search for blot in our database

SIGMA: The entire Sigma catalog on line. Search by name, number, chemical structure and any key word. All Biochemicals for any type of laboratory work. All types of PCR reagents (Taq, JumpStart Taq, RedTaq, AccuTaq, dNTPs, custom oligos etc) Sequencing reagents (gels, plates, clean-up kits) DNA and RNA purification kits (plasmid, genomic, etc) Recombinant protein expression (Trasnfection reagents, vectors, media, competent cells, purification supports, western blotting reagents etc) Cell culture reagents (media, fetal bovine serum) Cell signaling reagents (antibodies, reagents, kits)

Package inserts on-line. MSDS info on-line. Orders taken on-line - handled by your local Sigma-Aldrich office. Pricing on-line in most national currencies. Technical Services ("Ask a Scientist") on-line
Southern Blot: This is a brief overview of how a Southern blot (more formally called an DNA blot) is performed and what type of data you can obtain form one.
Stratagene - Eagle Sight software:
Direct imaging of nucleic acid or protein gels stained with ethidium bromide,
Coomassie® Blue, SYBR® Green or silver stains
Digital imaging of fluorescent TLC plate-based assays, blots or ELISA plates
Digital imaging of autoradiographs
Imaging and analyzing chemiluminescent samples
Colony and plaque counting
Syngene - Gene tools: Powerful analysis software for the analysis of 1D gels, spot blots, plates, colonies and autorads
YouTube: southern Blot: 1 minute video on Southern Blot

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