Dr Chromos tries again with fresh ampicillin, properly prepared plates, and a new batch of competent cells.

In the next experiment, there is a problem with the digestion with BamH1. Plate 4 has a lot of colonies. Of course in that case plate 8 also has a lot of colonies. There are a few white ones, but they will be difficult to pick up. Dr Chromo needs to buy some new Bam H1 (another £50 to spend on materials!)

Related Links

This is a search for transfo in our database

• Molecular Biology Protocols: Contains information about Buffers, RNA extractions, DNA techniques, Transformations, and Gels
• The photosynthetic process (article): This introductory chapter focuses on the structure of the photosynthetic machinery and the reactions essential for transforming light energy into chemical energy.
• Transformation of Competent cells: Transformation is the process of introducing foreign DNA (e.g plasmids, BAC) into a bacterium. Bacterial cells into which foreign DNA can be transformed are called competent.
• Tree inference at the T-REX server: The most comprehensive web server version includes methods for Visualization and interactive manipulation of phylogenetic trees (using Hierarchical, Radial and Axial types of drawing); Inference of phylogenetic trees using distance (NJ, BioNJ, UNJ, ADDTREE, MW, FITCH, Circular order reconstruction),parsimony (DNAPARS, PROTPARS, PARS, DOLLOP) and maximum likelihood (PHYML, DNAML, DNAMLK, PROML, PROMLK) based approaches; Inference of phylogenetic trees form incomplete distance matrices; Inference of phylogenetic networks (i.e. Reticulograms); Detection of horizontal gene transfers; Multiple sequence alignment (ClustalW), Sequence to distance transformations(using Uncorrected, Jukes-Cantor, Tajima-Nei, Kimura 2-parameter, Tamura, Jin-Nei gamma, Kimura protein, LogDet and F84 distances); Computation of the Robison and Foulds topological distance between two or more trees. The program can also carry out bootstrap and jackknife resampling to assess strength of support of the tree and network branches.