Products

RESearch

Copyright © 1995-1997 by John Antoniw

Overview

RESearch is a DNA sequence analysis Windows application for IBM compatible PCs. It has a user-friendly interface which enables you to analyse your DNA sequence with a minimum of effort.

It is now available from Amersham LIFE SCIENCE
catalogue number RPN 5300.


.

Quick summary of features

Edit and
manipulate
DNA
sequences


Edit DNA sequences

  • import GCG formatted sequences
  • insert sequences within vectors
  • reformat a sequence
  • reverse-complement a sequence


    • Search for
    nucleotide
    sequences


    Search DNA sequences

  • search for nucleotide motifs or PCR primer sites
  • make full use of nucleotide ambiguity codes for maximum
    flexibility
  • search in the forward and reverse-complement directions
  • search over the entire sequence or a sub-range
  • look for perfect matches or choose a number of mismatches
  • make progressive searches, increasing mismatches until a
    match is found
  • search with lists of predetermined nucleotide motifs
    or primers


    • Search for
    restriction
    enzyme sites


    Find restriction sites

  • use up-to-date information on over 400 restriction enzymes
  • select enzymes by:
    enzyme name (select by mouse click)
    type of ends produced, i.e. blunt, 5' or 3' overhang
    length of recognition sequence, i.e. 4-cutter, 6-cutter, etc.
    suppliers
  • search your entire DNA sequence or a sub-range
  • display enzymes that cut your sequence a certain number
    of times e.g. no cuts, one cut, 1-4 cuts
  • check for isoschizomers of any enzyme
  • find enzymes that produce compatible ends
  • easily find which companies supply the enzyme you want!


    • Investigate
    multiple
    restriction
    enzyme digests


    Multiple restriction digests

  • digest your DNA with as many enzymes as you like
  • select and deselect enzymes by a simple mouse click
  • predict the size and number of fragments produced
  • sort fragments by cut position and size for easy comparison
    with gels


    • Find
    open
    reading
    frames


    Find ORFs

  • display graphically open reading frames and
    all Met and Stop codons in all reading frames
  • select ORFs using the mouse and screen cursor;
    this automatically displays:
    nucleotide data of selected ORF
    amino acid sequence of encoded protein of selected ORF
    amino acid composition of encoded protein
    molecular weight of encoded protein


    • Search for
    amino acid
    sequences


    Search amino acid sequences

  • search any or all six reading frames
  • search over the entire sequence or a sub-range
  • look for perfect matches or a chosen number of mismatches
  • make progressive searches, increasing mismatches until
    a match is found
  • search with lists of predetermined amino acid motifs


    • Access
    built-in help


    Useful information

  • check nucleotide ambiguity codes,
    amino acid 1- and 3-letter codes,
    and the genetic code
  • datasheets for all Amersham restriction enzymes
  • a trouble-shooting guide for restriction enzyme problems
  • detailed on-line help explaining all features of the program


    • System
    requirements


    In order to use RESearch you will need
  • an IBM compatible PC with at least an 80386 microprocessor
  • Windows 3.1 or 3.11, Windows for Work Groups 3.11,
    or Windows 95.
  • a VGA monitor or better
  • at least 4 Mbytes RAM
  • at least 2 Mbytes of free hard disk space
  • a mouse or other pointing device


    • All enquiries to John Antoniw mail: john.antoniw@bbsrc.ac.uk

    Crop and Disease Management Dept., Rothamsted Research,
    Harpenden, Herts. AL5 2JQ

    Telephone: +44 1582 763133 - ext. 2428
    Fax: +44 1582 760981