BamHI
Origin: Isolated from Bacillus amyloliquefaciens H
Storage conditions: -20 °C
Storage buffer:
|
10
|
mM
|
Tris-HCl pH7.5
|
|
200
|
mM
|
NaCl
|
|
100
|
µM
|
EDTA
|
|
100
|
µg/ml
|
bovine serum albumin
|
|
10
|
mM
|
2-mercaptoethanol
|
|
0.15
|
%
|
Triton X-100
|
|
50
|
%
|
glycerol
|
Incubation conditions: Buffer K at 30 °C
Standard substrate: Lambda DNA
Unit definition
Relative activity in different buffers:
|
Buffer:
|
L
|
M
|
H
|
K
|
T+BSA
|
% Activity:
|
<20*
|
<20
|
40
|
100
|
<20*
|
* = star activity
Heat inactivation: Yes, 60 °C for 15 minutes
Ligation/recut efficiency:
After 10-fold overdigestion with this enzyme,
>95% of the DNA fragments could be ligated and
100% of these could be recut with this enzyme
Enforcement cloning tested
Catalogue numbers:
| E1010Y
|
5000
|
units
|
8-12
|
units/µl
|
| E1010Z
|
10000
|
units
|
8-12
|
units/µl
|
| E1010YH
|
5000
|
units
|
>40
|
units/µl
|
| E1010XH
|
50000
|
units
|
>40
|
units/µl
|
|
