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BioLab Help

Guided tour of BioLab
Getting Started
Description of BioLab
How do I?
Sample Experiments
Sample sequences available in BioLab
Advanced methods in BioLab

Full table of contents

Guided tour of BioLab

Getting Started

BioLab 's welcome dialog box
Your first ever experiment!
Disclaimers
Credits

Description of BioLab

• BioLab, the Virtual Laboratory
The Main Window

The Molecular Biology Lab
The Reaction Tubes

• Methods
  Basic Windows operations
  PCR
  Restriction Enzyme Digest
  Agarose Gel Electrophoresis
  Tube information pop-up
  ReL-label tubes
  Check Seqs (SeeMix)
  Hybridization of a blot
  
  
  Blotting a gel
  Making a DNA probe
  Hybridize the membrane with a probe
  Computing times

How do I?

Load an experiment?
Load an additional sample?
Save an experiment?
Transfer some DNA into a reaction tube?
Discard DNA?
Digest DNA?
PCR DNA?
Load a sample onto a gel?
Load a size marker onto the gel?
Change the agarose concentration of the gel?
Change the length of the gel?
Change the RunTime of the gel?
Blot a gel?
How do I cut a gel slice? (Prepare a probe)
Load a probe?
Hybridize a blot?
Import a DNA sequence into BioLab?
Create my own DNA?

Sample experiments

• Electrophoresis of DNA
  • Expt001 : Electrophoresis of a single DNA fragment
  • Expt002 : Electrophoresis of a mixture of DNA fragments
  • Expt003 : Using size markers
  • Expt004 : Effect of time on separation
  
  
• Restriction Enzyme Digest
  • Expt005 : Restriction Enzyme Digest
  • Expt006 : A choice of restriction enzymes
  • Expt007 : Double cut of a linear fragment
  • Expt008 : Restriction Map (difficult)
  • Expt014 : Restriction Digest of the Bacteriophage Lambda (with SAPS)
  
  
• Southern Blot
  • Expt009 : Making a DNA probe, Blotting a gel and hybridization
  
  
• Diagnostic
  • Expt010 : Diagnosis of sickle-cell anemia
  • Expt011 : Diagnosis of haemophilia
  • Expt013 : Diagnosis of Cystic Fibrosis
  
  
• PCR
  • Expt012 : Introduction to PCR
  • Expt011 : Diagnosis of Cystic Fibrosis (Uses a PCR diagnostic)

Sample sequences

Advanced methods in BioLab