The Molecular Biology Notebook OnlineYou are here: Biotechnology
Genetic Engineering
Introduction
Carefully selecting seeds for planting the next year, preventing
a weak bull from breeding, and so on, are common and old practices. They
lead to the improvement of the gene pool.
With the increase in the knowledge of molecular biology, and the decoding
of the human genome
for example, new tools are being introduced. It is
now possible to carefully select a gene in the genome of an individual,
and insert it in the genome of another, enabling this transfered gene to
be expressed.
What is needed
- A sound knowledge of DNA : its structure and associated proteins.
- Methods used in Molecular Biology
- Microorganisms.
You might need to go back to some of the preceding chapters to have a clearer idea.
In this chapter on the Applications of Molecular Biology, some of the basic techniques used in any molecular biology laboratory are presented. The techniques are sorted under the heading 'Genetic mapping', as most of them are used in an attempt to map genes!
- Preparation of DNA
- Electrophoresis
- Blotting and hybridisation
- PCR
- Sequencing
Another section, under the heading 'Gene cloning technology', deals with the recombination of DNA, i.e. the use of bacteria, or other organisms, to produce DNA.
In conclusion, a chapter is devoted to some examples of the use of these technologies in real life experiments.
Tweet
Genetic Engineering on Wikipedia
Genetic Engineering at Answers. com
Related Links
-NCBE GM food guideThis guide has been put together in response to the intense interest in food biotechnology in the
New Links
-Running DeCypher from Geneious
There is a plugin to connect Geneious and DeCypher together. This page explains how to set it up, an...
-DeCypher on G1
This is the first instance of DeCypher on a G1 card. The oldest. ...
-Decypher on J1
The latest instance (testing). ...
-Decypher on H1
Intermediate DeCypher instance: requires username and login....
-Laboratory Information Management Systems for DNA Barcoding
Here, we demonstrate an example workflow of a specimen’s entry into the LIMS database to the publis...
