People › Dr Angela Karp
Angela Karp is Scientific Director of the Rothamsted Centre for Bioenergy and Climate Change. Her research focuses on optimising perennial biomass crops (especially willows) for bioenergy and biofuels. Amongst many projects, Dr Karp is responsible for the UK willow breeding programme and also coordinated a RELU-Biomass project on the social, environmental and economic implications of increasing land use under energy crops. She now leads the BSBEC-BioMASS Programme - one of six research hubs comprising the BBSRC Sustainable Bioenergy Centre (http://www.bsbec.bbsrc.ac.uk/). In 2007 she received the RASE Research Medal and, in 2008, the Alfred-Toepfer prize for research achievements in willow.
Principal Investigator› Ionic liquid biorefining of lignocellulose to sustainable polymers
› Logistics for energy crops′ biomass - LOGISTEC
Project Leader› Accelerating breeding for biomass yield in short rotation coppice willow by exploiting knowledge of shoot development in Arabidopsis
› Maximising carbon retention in soils
› The BBSRC Sustainable Bioenergy Centre (BSBEC): Perennial Bioenergy Crops Programme
Member› Integrating carbon systems
› Maximising carbon harvest from perennial crops
Principal InvestigatorA whole-system approach to analysing bioenergy demand and supply: mobilising the long-term potential of bioenergy TSEC-BIOSYS
The specific research objectives are to:
The project will determine if economic production of viable seed of reed canary grass and switchgrass is possible in the UK.
The aim of this project is to develop a Bayesian inference programme for making inferences about the genealogy of selfing lines and the outcrossing rate, from multi-locus genotypic data.
Willow (Salix spp) grown as Short Rotation Coppice (SRC) is one of the main biomass crops in the UK and has been identified as appropriate for the production of biomass energy in the Palearctic and North America due to its potential for rapid growth in temperate climates. It serves as a host for several insect species, many of which are potential pests. The latter includes the giant willow aphid (Tuberolachnus salignus (Gmelin)), which often occurs at high density.
The overall aim of this project is to develop and apply models for soil-plant-atmosphere interactions to assess productivity and impacts of new cropping systems under climatic variability (global change). These will operate over different scales and complexities. Particular focus will be on finding low input optima for maximising productivity from perennial bioenergy crops whilst minimising costs and environmental impacts. Concepts established for arable crops are being expanded to biomass crops, e.g. rhizomatous grasses. Initially, emphasis is given to quantifying crop responses to water stress and temperature, and to evaluating different crop growth strategies (evasion, enhanced exploration, recycling of reserves).
Genetic diversity provides us with "markers" which can be used to illuminate population processes such as migration, population growth and decline, extinction and recolonisation. We are also interested in the contribution of different genotypes to population growth and decline, and hence adaptation.
Within this project Rothamsted Research is responsible for an investigation of the role of agronomy in determining yield and quality of three perennial grasses (Panicum virgatum, Phalaris arundinacea and Miscanthus x giganteus) grown as bio-fuels. These crops can achieve their yield potential with the addition of fertiliser, but those fertilisers could affect the product quality in a negative way. The concentration of the following elements in the bio-fuel should be minimised to maximise product quality; concentration directly influenced by fertiliser practice; N, K, S and Cl, indirectly influenced by fertiliser practice Na, Ca and Mg and water content.
A recent evaluation of trials at Rothamsted concluded that the most promising energy grasses for use as biofuels in the UK are Miscanthus, switchgrass and reed canary grass. The grasses were found to be comparable in performance to short rotation coppice (SRC). Fuel and ash characteristics showed the three grasses to have similar characteristics to straw and suitable for combustion in a modern combustion plant. In this project switchgrass and reed canary grass will be evaluated at nine locations throughout the UK to establish their yield under various soil and climatic conditions. Miscanthus ,of which more is known, will be used as the benchmark crop at each site.Growing energy in the Atlantic Area: opportunities for large scale development
Five genotypes of Miscanthus are being evaluated in a fully replicated and randomised design. This work is additional to an existing project Superglen (project no. 4602)Integrated control of fungal diseases in willows and poplars for bioenergy
The main objective of the research is to provide underpinning science for the implementation of an integrated, non-chemical strategy of disease management in UK renewable energy crop production.
Rust caused by Melampsora is the most serious problem of willow (Salix), the main crop for renewable energy and important vegetation for conservation. Economic and environmental considerations rule out the use of fungicide for rust control. This project is to develop integrated rust control measures, without inputs of fungicide by addressing population biology and epidemiology of Melampsora, sources and genetics of rust resistance in Salix, function of host mixtures against rust and the potential of using a mycoparasite for biological control. The project will provide measures towards breeding for durable resistance, designing optimum mixtures and deploying the biocontrol agent to suppress rust disease.
Theme 1: Resources (Theme Leader: Rothamsted Research)
Molecular marker techniques will be used to determine the genetic identity of introgressed populations of polyploid plants. Each EU partner will use a different technique: AFLPs, RAPDs and enzyme consensus primers, nuclear SSRs, cpDNA and mtDNA sequence analyses and cpSSRs. Salix (willow) species and hybrids at a European scale will be used as the model plants. There are three major activities: (i) the application (and further development) of molecular techniques on intra- and inter-specific controlled crosses and reference germplasm, constituting the test system (S. alba, S. fragilis and their hybrids); (ii) data analysis and evaluation of each technique in their ability to genotype individuals, reveal hybrid identity and to elucidate polyploid heterozygosity and introgressive hybridisation; (iii) generation of guidelines for restoration objectives of natural and semi-natural willow-dominated areas. Year 1 objectives: DNA extractions and screening of full-sib progenies and reference material from germplasm collections. Forestry Institutes and Breeding Stations (including IACR-Long Ashton) will supply plant material. The major problem to address will be the polyploid nature of the genomes. Year 2 and 3 objectives: an in-depth study of natural hybrid populations along stretches of major European rivers using the most appropriate molecular techniques. A major problem to overcome will be the dynamic nature of the hybrid complex.
A process based water balance model able to estimate the water use for a range of land covers and the full range of climates will be used to estimate the water use of future plantations of energy crops. Some of the parameter values for the model will be measured in established and recently planted energy crops at Rothamsted and Woburn.
Genetic improvement programmes in Sweden and the UK have made significant progress in breeding Short Rotation Coppice (SRC) willow. However, to expand production, cultivars suited to a wider range of European environments and future climates will be needed. The project will address this by delivering an understanding of the genetic basis of yield in the context of varied European environments into breeding programmes and provide molecular tools for selection. The research will focus on a Salix viminalis association mapping population generated from the unique germplasm resources held by Rothamsted Research and Swedish partners. After first assessing population structure in the germplasm, suitable material (~400 genotypes) will be planted at seven contrasting sites across Europe and key biomass-related traits will be assessed. Comparative trait data will be used in candidate gene-based association mapping to identify favourable alleles that will be delivered to breeding programmes for the development and deployment of molecular based selection strategies. Focus will be given to traits that are not yet will-studied in national programmes but are of importance, namely achievement of high biomass yield on marginal land and in conditions where water may be limiting. For growth on marginal land, novel microarray studies will be performed to identify candidate genes involved in this trait. To study yield in the context of future climate conditions, candidate genes for drought-related will be selected from our QTL and from the available literature. A similar approach will be taken for phenology traits. A significant component of the research comprises the analysis and transfer of existing research outputs to support this project and the development of willow in partner countries. Furthermore, active knowledge management and stakeholder interaction will ensure delivery into practice and provide an accessible legacy for efficient public research in future.Willows and Energy Grass Collection
Short rotation coppice willow is already being grown as an energy crop in parts of the UK but crop breeding has the potential to greatly improve yields, making it economically viable for a much wider range of growers. Rothamsted’s research on bio energy is aimed at helping to bring about these improvements. Central to this work is a unique resource; the National Willows Collection. This is a repository for willow germplasm, set up in the 1920s as a way of conserving varieties which were being lost when rural crafts such as basket and hurdle-making declined. Today it is maintained on the Rothamsted farm and contains around 1,300 accessions. It forms the basic resource for the Defra funded ‘Begin’ programme (Improving short rotation coppice through breeding and genomics) and a significant resource. for the EraNet project (Targeted breeding of a European SRC willow crop for diverse environments and future climates) and the BBSRC Crop Science Initiative project 'Accelerating breeding for biomass yield in short rotation coppice willow by exploiting knowledge of shoot development in Arabidopsis'.
Project LeaderBBSRC Industrial CASE: Carbon and nitrogen recycling in Short Rotation Coppice willow in relation to development and biomass composition
Willows are among the most advanced biomass crops in temperate regions. They can accumulate biomass rapidly due to an effective growth strategy which relies on rapid mobilisation of resources stored over the winter enabling simultaneous development of many shoots from each stool in the spring. As the stems grow larger and their leaves mature, they become independent of reserves and eventually the flux is reversed and resources are exported from the leaves to other parts of the plant. The balance between new growth and recycling is a crucial factor for willow. Studies of different Short Rotation Coppice (SRC) cycles and planting densities have defined the shortest number of years between harvests which enable sufficient root reserves to build up and the highest densities possible for reducing competition between willow stools. However, at present, little is known about how N recycling changes during the SRC cycle and over successive SRC cycles. These are important issues to study since the positive greenhouse gas emission and energy balances from crop to fuel result from the low-input nature of their cultivation and specifically the low use of N. Yield improvement gains thus must be achieved without increasing the requirements for nutrients. The composition of willow stem biomass will also affect the efficiency of energy conversion. This project will address these knowledge gaps by studying N (and to some extent C) in willow in different tissues and at different stages in the SRC cycle and relating this to biomass composition and biofuel productivity. This project will take advantage of existing willow material including the K8 population in which QTL for biomass yield have been mapped. In addition, we will utilise standard material (varieties) in which the same genotype is available at different SRC cycle stages within one year.Biodiversity impacts of Miscanthus and short rotation coppice (SRC) willow at a landscape scale
Biomass crops, principally short-rotation coppice (SRC) willow (Salix spp) and Miscanthus grass (Miscanthus x giganteus), are recognised as playing an important role in the UK Government’s commitment to increasing energy security and reducing greenhouse gas emissions. Land conversion to such crops is predicted to expand significantly. Compared with arable crops, biomass crops typically require low inputs, however, they are perennial and much taller than traditional arable crops, remain in the ground for long periods (up to 25 years) and are harvested in late winter/early spring. These factors have implications for the visual appearance and character of a landscape, for farm and tourist income, and for the hydrology and biodiversity. An ongoing RELU funded project on the social, environmental and ecological implications of increasing rural land use under energy crops aims to develop a sustainability appraisal of conversion of land to biomass crops. Although it provides a comprehensive platform upon which to assess the implications of increasing land use under energy crops not all aspects on biodiversity could be covered. The FarmScale Evaluations (FSE) of genetically modified herbicide tolerant crops showed that management should be optimised to assure the highest biodiversity attainable. For biomass crops, this includes the scales of growing within a landscape, and temporal effects including the crop age, time in the cutting cycle and timing of cutting. This project aims to expand the evidence base on biodiversity by:
The main aim of this project is the development of perennial biomass crops as a source of renewable energy within a sustainable land-use context.
Alien invasive plants such as Heracleum mantegazzianum are having a severe impact on biodiversity in Europe but no sustainable solutions are available to stop their spread and prevent future invasions. The overall objective of the project is to develop an integrated management strategy that comprises effective, practicable and sustainable means of controlling an alien non-agricultural weed (H. mantegazzianum). This will provide a generic control strategy to safeguard the biodiversity of Europe from the increasingly serious threat of other alien invasives. In addition to the effective control of this species, a concept would be produced which could serve as a template by which other exotic species could be controlled or prevented from reaching the invasive phase.
This project aims to deliver the breeding programme and plant materials for further improvement of willows as an energy crop. A breeding programme that draws from previous and ongoing molecular marker and genetic mapping research will be developed to produce elite genotypes with high yields and resistances. Crossing will make intelligent use of the germplasm within the National Willow Collection. Selection strategies for yield will be improved. Attempts will be made to overcome existing crossing barriers and water-use efficiency and/or are drought tolerance will be explored. The cost-effectiveness of marker-assisted selection (MAS) will be evaluated. Genes with major effects on key agronomic traits will be identified through a genomics-based approach, which exploits recent developments in poplar. We have already identified markers for QTLs conferring rust resistance, susceptibility to invertebrate pests and major components of yield. We will confirm the robustness of these QTLs by comparative assessments at the Long Ashton and Rothamsted sites. We will exploit poplar genomics, to identify candidate genes for development of EST-based markers in willow and map these on the existing K8 willow map to identify those showing co-location with QTL. A BAC library will also be constructed. The locations of QTL known to determine yield in poplar will be compared with willow to determine ‘robust’ SRC yield QTL. From the available ESTs, and from on-going research on gene expression using microarrays, candidates for yield traits will be identified and mapped using a SNPs-based approach. The physical sequence of poplar, (available in 2004/5) will be used, with focus on ESTs of interest from microarrays, to detect candidate genes from blast searches and their physical locality on the poplar genome map will be determined. A large and diverse collection of Populus nigra will be used in a linkage disequilibrium study to confirm candidate-QTL associations for yield.Improving short rotation coppice through breeding and genomics (Extension)
This project is a 6 month extension to the Defra sponsored project 'Improving short rotation coppice through breeding and genomics' which aims to deliver the breeding programme and plant materials for further improvement of willows as an energy crop. A breeding programme that draws from previous and ongoing molecular marker and genetic mapping research will be developed to produce elite genotypes with high yields and resistances. Crossing will make intelligent use of the germplasm within the National Willow Collection. Selection strategies for yield will be improved. Attempts will be made to overcome existing crossing barriers and water-use efficiency and/or drought tolerance will be explored. Genes with major effects on key agronomic traits will be identified through a genomics-based approach, which exploits recent developments in poplar. We have already identified markers for QTLs conferring rust resistance, susceptibility to invertebrate pests and major components of yield. We will confirm the robustness of these QTLs by comparative assessments at the Long Ashton and Rothamsted sites. We will exploit poplar genomics, to identify candidate genes for development of EST-based markers in willow and map these on the existing K8 willow map to identify those showing co-location with QTL. A BAC library will also be constructed. The locations of QTL known to determine yield in poplar will be compared with willow to determine ‘robust’ SRC yield QTL. From the available ESTs and from on-going research, candidates for yield traits will be identified and mapped using a SNPs-based approach. The physical sequence of poplar will be used, with focus on ESTs of interest from microarrays, to detect candidate genes from blast searches and their physical locality on the poplar genome map will be determined. A large and diverse collection of Populus nigra will be used in a linkage disequilibrium study to confirm candidate-QTL associations for yield.Improving willow breeding efficiency for biomass through the implementation of molecular marker technologies
The aim of this project is to improve willow breeding efficiency through the implementation of molecular marker technologies. These technologies will be used to map and locate genes controlling agronomic traits and to provide markers linked to the traits for marker assisted selection. A first genetic map of willow will be constructed and attempts to identify markers which are linked to the rust resistance genes initiated. Emphasis will be placed on locating and establishing the genetic basis of three main agronomic traits in willow (rust resistance, beetle resistance and yield) and identifying linked markers for more efficient selections. Two work strategies will be pursued: (i) reference crosses will be established for trait assessment using both phenotypic (field assessed) and molecular marker assays (ii) the existing willow map will be enriched to improve density and coverage of markers and poplar markers will be added to the willow map. The establishment of reference families will provide the means for genetic analysis of agronomic traits. Emphasis will be placed on establishing the genetic basis of rust resistance, Chrysomelid beetle resistance and yield. The physiological basis of biomass yield will be studied in selected lines in collaboration with the University of Southampton. These families will also provide a standard resource for studies of various other aspects of willows. The advanced genome mapping of willow and identification of linked markers will aid marker assisted selection and accelerate breeding efforts to achieve higher biomass yield production. Merging of the willow and poplar maps will increase the number of markers available in willow for marker-assisted backcross and introgression selections and increase the chances of finding markers linked to agronomic traits for marker assisted selection.Optimising the development of the energy grass Miscanthus through manipulation of flowering time
Biomass from energy crops are an important part of the renewable energy mix. Miscanthus is a perennial grass which combines the fast growth rate of a tropical grass with a tolerance to grow at UK temperatures. It requires little to no fertiliser or herbicide inputs and produces a high yield of biomass every year. However as Miscanthus is a new crop, previous research has been extremely limited. This proposal seeks to start addressing this deficit by investigating the molecular basis of flowering by exploiting knowledge in model organisms such as Arabidopsis thaliana, rice and maize.
The largest threat to renewable energy production from short rotation coppice (SRC) willows is the propensity for plantations to succumb to pests and diseases. This project aims to provide a firm scientific basis for the use of a non-chemical approach to the control of vertebrate and invertebrate pests of SRC willows. The approach seeks to identify and exploit the interactions between pest herbivores and the biological chemistry of the willow. Depending upon the specific compounds that are present, pest herbivores are either attracted or repelled from feeding on the willow and this may provide the basis of a pest management strategy that is reliant on the willow’s own natural defences and not on the application of pesticides. Such a strategy would be based on the use of different willow varieties which differ in their attractiveness or repulsion to pests. There are parallels between the sensory acuities of insects and mammals, and the strategy could be targeted against pest species of both groups. In the first instance, the work will focus on Phratora vulgatissima (the blue willow beetle) as the invertebrate pest and rabbit as the vertebrate pest. In future projects, the work should be extended to other major pest species of SRC willows. In this project, experiments will be conducted to establish: (i) the identification of the specific biological compounds in the willow host to which beetles and rabbits respond to (2) variation in the willow hosts with respect to these compounds both among varieties and for the same variety in different environmental conditions (i.e. different localities in the UK) and (3) variation in the beetle populations with respect to their genetic composition (assessed using microsatellite markers), overall morphology and feeding preferences. This information is needed before a second phase of the work can be pursued.Population genetics of herbicide resistance in grass-weeds
The evolution of herbicide resistance offers exciting opportunities for fundamental research on the origins, selection and spread of adaptive traits in weed populations. This project will build on recent advances in understanding the mechanism of resistance to herbicides inhibiting the enzyme acetylcoenzyme A carboxylase (ACCase) in grass-weeds. It will exploit a combination of bioassays, biochemical assays and molecular approaches to gain further insights into how mutation at the ACCase locus affect the expression and dominance of resistance, and use neutral genetic markers to investigate the 'phylogeny' and dynamics of resistance genes in field populations. Candidate genes for other potentially adaptive traits will also be studied as they become available from genomic studies in grass and other plant genomes.Social, Economic and Environmental Implications of Increasing Rural Land Use under Energy Crops
Energy crops, such as short rotation coppice (SRC) willow and Miscanthus, can contribute to UK Government’s commitment to reduce CO2 emissions. Under policy support, energy crops may cover a greater land area in future. Although some potential impacts of converting land to energy crops have been researched it is not yet clear how to balance decisions based on climate, soil and water availability, against possible impacts on the environment, social acceptance and rural economy. Using the East Midlands and South-West regions as study areas, this interdisciplinary project reviews current knowledge and conducts new state-of-the-art social, economic, hydrological and biodiversity research to develop an integrated scientific framework for Sustainability Appraisal (SA) of the medium and long term conversion of land to energy crops. Implementation of the SA framework will be evaluated and the most appropriate planting scenarios identified. Scientific guidance for updating Best Practice Guides and scientific tools for Environmental Impact Assessments, Strategic Environmental Assessments or SAs involving projects, policies or programmes where increased planting of energy crops is proposed or anticipated, will be provided. Stakeholder involvement is integral in the approach. The results will benefit farmers, energy producers, land planners, regional development agencies, policy makers, environmental agencies and the public.UK-Brazil research on second generation biofuels
The project covers costs related to the travel and living expenses of 15 scientists from Embrapa and its partner R&D institutions of the Brazilian National System of Agricultural Research (SNPA). The scientists will spend up to 6 months in a UK institution within the BBSRC Sustainable Bioenergy Centre (BSBEC) but will continue to be employees of their institutions of origin (Embrapa or its partner R&D institutions of the SNPA). In this context, the main activities for this project are:
In this project molecular genetic and ecological approaches are being used to determine the processes that act upon the genetic diversity and ecological dynamics of plants as components of the agroecosystem. In agriculture, cultivated and natural species exist in a mosaic of fields and fragmented natural or semi-natural habitat. The growth, productivity and quality of products derived from crops are influenced both by the genetic make-up and management of the crop and by the way that the crop interacts with the environment and other species in the agro-ecosystem. Knowledge of the population structure and ecological dynamics of plants is key to sustainable agriculture and conservation of biodiversity.
MemberBBSRC SABR Studentship: An integrated bioinformatics approach for identifying genes that influence energy crop architecture
The aim of the project is to create an ONDEX database application and user software that will enable users in the Karp laboratory to explore the evidence linking genes found under a QTL and to prioritise them according to the evidence supporting their potential role in determining architecture and other traits under investigation for the genetic improvement of bioenergy crops.
This project aims to gain a better understanding of the population dynamics of resistant and susceptible populations of the rye grass Lolium multiflorum so that improved strategies for control of this weed can be devised. Specific objectives are:
The pieces of equipment to be purchased from this grant are three LICORs and a liquid handling robot.
Full publication list
Karp, A. & Jones, R.N. (1981) Variation in Chiasma distribution in inbred perennial ryegrass. Heredity 47: 152-152.
Karp, A. & Jones, R.N. (1982) Cytogenetics of Lolium perenne. Part 1. Chiasma frequency variation in inbred lines. Theor. Appl. Genet. 62: 177-183.
Karp, A., Nelson, R.S., Thomas, E. & Bright, S.W.J. (1982) Chromosome variation in protoplast-derived potato plants. Theor. Appl. Genet. 63: 265-272.
Karp, A., Rees, H., Jewell, A.W. (1982) The effects of nucleotype and genotype upon pollen development in Hyacinth and Scilla. Heredity 48: 251-261.
Karp, A. & Jones, R.N. (1983) Cytogenetics of Lolium perenne. Part 2. Chiasma distribution in inbred lines. Theor. Appl. Genet. 64: 137-145.
Karp, A. & Jones, R.N. (1983) Cytogenetics of Lolium perenne. Part 3. Correlation between chiasmata and U-type exchange. Theor. Appl.Genet. 65: 149-156.
Ooms, G., Karp, A. & Roberts, J. (1983) From tumour to tuber; tumour cell characteristics and chromosome numbers of crown-gall-derived tetraploid potato plants (Solanum tuberosum cv. Maris Bard). Theor. Appl. Genet. 66: 169-172.
Karp, A. & Maddock, S.E. (1984) Chromosome variation in wheat plants regenerated from cultured immature embryos. Theor. Appl. Genet. 67: 249-255.
Karp, A., Risiott, R., Jones, M.G.K. & Bright, S.W.J. (1984) Chromosome doubling in monohaploid and dihaploid potatoes by regeneration from cultured leaf explants. Plant Cell Tissue Organ Culture 3: 363-373.
Burrell, M.M., Twell, D., Karp, A. & Ooms, G. (1985) Expression of shoot-inducing Ti TL-DNA in differentiated tissues of potato (Solanum tuberosum c.v. Maris Bard). Plant. Mol. Biol. 5: 213-222.
Cresissen, G.P. & Karp, A. (1985) Karyotypic changes in potato plants regenerated from protoplasts. Plant Cell Tissue Organ Culture 4: 171-182.
Jones, R.N. & Karp, A. (1985) ‘Jumping genes in maize.' J. Agric Science 65: 79-108.
Miflin, B; Jones, M; Ooms, G; Karp, A; Maddock, S; Bright, S. (1985) Novel ways of manipulating plants. Journal of the Science of Food and Agriculture 36 (8): 651-652.
Ooms, G; Bains, A; Burrell, M; Karp, A; Twell, D; Wilcox, E. (1985) Genetic manipulation in cultivars of oilseed rape (Brassica napus) using agrobacterium. Theoretical and Applied Genetics 71 (2): 325-329.
Ooms, G., Karp, A., Burrell, M.M., Twell, D. & Roberts, J. (1985) Genetic modification of potato development using Ri-T-DNA. Theoretical and Applied Genetics 70: 440-446.
Wheeler, V.A., Evans, N.E., Foulger, D., Webb, K.J. Karp, A. Franklin, J. & Bright, S.W.J. (1985). Shoot formation from explant cultures of fourteen potato cultivars and studies of the cytology and morphology of regenerated plants. Ann. Bot. 55: 309-320.
Fish, N. & Karp, A. (1986) Improvements in regeneration from protoplasts of potato and studies on chromosome stability 1.The effect of initial culture media. Theor. Appl. Genet. 72: 405-412.
Nelson, R.S., Karp, A. & Bright, S.W.J. (1986) Ploidy variation in Solanum brevidens plants regenerated from protoplasts using an improved culture system. J. Exp. Bot. 37: 253-261
Ooms, G., Bossen, M.E., Burrell, M.M. & Karp, A. (1986) Genetic manipulation in potato using Agrobacterium rhizogenes. Potato Res. 29: 367-379.
Breiman, A., Rotem, D., Karp, A. & Shaskin, H. (1987) Heritable somaclonal variation in wild barley ( Hordeum spontaneum ). Theor. Appl. Genet. 74: 104-112.
Fish, N., Karp, A. & Jones, M.G.K. (1987) Improved isolation of dihaploid Solanum tuberosum protoplasts and the production of somatic hybrids between dihaploid S. tuberosum and S. brevidens. In: Vitro Cell Devel. Biol. 23: 575-580.
Karp, A., Jones, M.G.K. Ooms, G., Bright, S.W.J. (1987) Potato protoplasts and tissue-culture in crop improvement. Biotechnology & Genetic Engineering Reviews 5: 1-32.
Karp, A., Steele, S.H., Breiman, A., Shewry, P.R.S., Parmar, S. & Jones, M.G.K. (1987) Minimal variation in barley plants regenerated from cultured immature embryos. Genome 29: 405-412.
Karp, A., Wu, Q.S. & Jones, M.G.K. (1987) Cytology of wheat suspensions and cultured protoplasts. Annual Wheat Newsletter. 33: pp 102.
Karp, A., Wu, W.S., Steele, S.H. & Jones, M.G.K. (1987) Chromosome variation in dividing protoplasts and cell suspensions of wheat. Theor. Appl. Genet. 74: 140-146.
Ooms, G., Burrell, M.M., Karp, A. Bevan, M. & Hille, J. (1987) Genetic transformation in two cultivars of potato with T-DNA from disarmed Agrobacterium. Theor. Appl. Genet. 73: 740-755.
Shewry, P.R. Tatham, A.S., Karp, A. & Jones, M.G.K. (1987) Improvement of the seed protein-quality of barley + wheat by genetic-engineering. Cereal Foods World 32 (9): 674-674.
Bebeli, P., Karp, A., Kaltsikes, P.J. (1988) Plant regeneration from cultured immature embryos of sister lines of rye and triticale differing in their content of heterochromatin 1. Morphogenetic response. Theor. Appl. Genet. 75: 929-936
Karp, A., Wu, Q.S. Steele, S.H. & Jones, M.G.K. (1988) Chromosome variation in dividing protoplasts and cell suspensions of wheat. Annual Wheat Newsletter 34: pp 9.
Lee, B.T., Murdoch, K., Topping, J., De Bothe M.T.J., Wu, Q.S., Karp, A., Steele, S., Symonds, C., Kreis, M. & Jones, M.G.K. (1988) Isolation, culture and morphogenesis from wheat protoplasts and study of expression of DNA constructs by direct gene transfer. Plant Cell Tissue and Organ Culture 12: 223-226.
Pehu, E., Potter, R., Karp, A. & Jones, M.G.K. (1988) Variation in chromosome-number in somatic hybrids of dihaploid potato Solanum tuberosum and Solanum brevidens. Physiologia Plantarum 73 (2): A22-A22.
Cannell, M., Mulchany M., Karp, A. & Shewry, P.R. (1989) Chromosomal assignment of characterised cDNA's and genes using RFLP analysis of ditelosomic addition lines. Barley Genet. Newsl. 19: 9-11.
Clark, M., Karp, A. & Archer S.A. (1989) Physical mapping of the B-hordein loci on barley chromosome 5 by in situ hybridisation. Genome 32: 925-929.
Fish, N., Karp, A. & Jones, M.G.K. (1989) Production of somatic hybrids by electrofusion in Solanum. Theor. Appl. Genet. 76: 260-266.
Jones, H., Karp, A. & Jones, M.G.K. (1989) Isolation, culture and regeneration of plants from potato protoplasts. Plant Cell Reports 8: 307-311.
Karp, A., Jones, M.G.K., Foulger, D., Fish, N. & Bright, S.W.J. (1989) Variability in potato tissue culture. Amer. Potato J. 66: 669-684.
Pehu, E.P., Karp, A., Moore, K., Steele, S.H., Dunckley, R. & Jones M.G.K. (1989) Molecular, cytogenetic and morphological characterisation of somatic hybrids of dihaploid Solanum tuberosum and diploid Solanum brevidens. Theor. Appl. Genet. 78: 696-704.
Bebeli P., Karp, A. & Kaltsikes, P.J. (1990) Somaclonal variation from cultured immature embryos of rye differing in heterochromatic content. Genome 33: 177-183.
Karp, A. (1990) Somaclonal Variation in Plants. In: Journal of the Agricultural Society 70: pp 67-83.
Pehu, E.P., Gibson, R.W., Jones M.G.K. & Karp, A. (1990) Studies on the genetic basis of resistance to Potato Leaf Roll Virus, Potato Virus Y and Potato Virus X in Solanum brevidens using somatic hybrids of S. brevidens and S. tuberosum. Plant Sci. 69: 95-101.
Pehu, E., Thomas, M., Poutala, T., Karp, A. & Jones, M.G.K. (1990) Species specific sequences in the genus Solanum: identification, characterization and application to study somatic hybrids of S. brevidens and S. tuberosum. Theor. Appl. Genet. 80: 693-698.
Cannell, A., Karp, A., Isaac, P. & Shewry, P.R. (1991) Chromosomal assignment of genes in barley using telosomic wheat-barley addition lines. Genome 35: 17-23.
Karp, A., Owen, P., Steele, S.H., Bebeli, P.J., Kaltsikes, P.J. (1992) Variation in telomeric heterochromatin in somaclones of rye. Genome 35: 590-593.
Bebeli, P.J., Kaltsikes, P.J. & Karp, A. (1993). Field evaluation of somaclonal variation in rye lines differing in telomeric heterochromatin. J. Genetics and Breed 47: 15-22.
Blunden, R., Wilkes, T.J., Forster, J.W. Sandery, M.J., Karp, A. & Jones, R.N. (1993) Identification of the E3900 family, a second family of rye B-chromosome specific sequences. Genome 36: 706-711.
Karp, A (1993) Are your plants normal? Somaclonal variation in regenerated and transgenic plants. Agro Food Industrial Food Hi-Tech. 4: 7-12.
Poulimatka, M. & Karp, A. (1993) Meiotic disturbances resulting from tissue culture of inbred and outbred rye. Heredity 71, 138-144.
Winfield, M., Davey, M.R. & Karp, A. (1993) A comparison of chromosome instability in cell suspensions of diploid, tetraploid and hexaploid wheats. Heredity 70: 187-194.
Xu, Y.S., Jones, M.G.K., Karp, A. & Pehu, E. (1993) Analysis of the mitochondrial DNA of the somatic hybrids of Solanum brevidens and Solanum tuberosum using non-radioactive digoxigenin-labelled probes. Theor Appl Genet 85: 1017-1022.
Gecheff, K., Georgiev, S., Wilkes, T., Hvarleva, T. & Karp, A. (1994) Cytological and molecular evidence of deletion of ribosomal RNA genes in chromosome 6 of barley (Hordeum vulgare L ) Genome 37: 419-425.
Iingram, D.S. & Karp A. (1994) Biotechnology Foresight and Biodiversity - Time for Stewardship. Biologist 41: pp 186.
Winfield, M.O., McMaster, T.J., Karp, A & Miles, M.J. (1994) Atomic force microscopy of plant chromosomes. Chromosome Res. 3: 128-131.
Karp, A. (1995) Somaclonal variation as a tool for crop improvement. Euphytica 85: 295-302.
Karp, A. & Ingram, D.S. (1995) Biotechnology, Biodiversity and Conservation. Bio/technology 13, 522.
Wilkes, T. M., Francki, M.G., Langridge, P., Karp, A., Jones, R.N. & Forster, J.W. (1995) Analysis of rye B-chromosome structure by the use of fluorescence in situ hybridisation (FISH). Chromosome Res. 3: 466-472.
Winfield, M.O., Karp, A., Lazzeri, P.A. & Davey, M.R. (1995) Instability in chromosome 5D in cell lines of Triticum tauschii and heritable variation in the regenerated plants. Genome 38: 737-742.
Winfield, M.O., Schmidt, M., Davey, M.R., Lörz, H. & Karp A (1995) Non-random chromosome variation and morphogenic potential in cell lines of breadwheat (Triticum aestivum). Genome 38: 869-878.
Edwards K.J., Barker A., Daly, A., Jones, C. & Karp, A. (1996) Microsatellite Libraries Enriched for several Microsatellite Sequences in Plants. BioTechniques 20: 758-759.
Hartmann, C., Winfield, M.O., Corre, F., Davey, M.R., Rode, A. & Karp, A. (1996) A comparative study of the mitochondrial genome organization in in vitro cultures of di-, tetra- and hexaploid Triticum species. Theor. Appl. Genet. 93: 968-974.
Karp, A, Seberg, O. & Buiatti, M. (1996) Molecular Techniques in the Assessment of Botanical Diversity. Ann. Botany 78: 143-149.
McMaster, T.J.; Winfield, M.O.; Karp A. & Miles, J. (1996) Analysis of cereal chromosomes by atomic force microscopy. Genome 39: 439-444.
McMaster, T.J., Winfield, M., Baker, A.A., Karp, A. & Miles, M.J. (1996) Chromosome classification by atomic force microscopy volume measurement. J. Vac. Sci. Technol. B14: 1-5.
Beismann, H., Barker J.H.A., Karp, A. & Speck, T. (1997) AFLP analysis sheds light on distribution of two Salix species and their hybrid along a natural gradient. Mol. Ecol. 6: 989-993.
Jones, C.J., Edwards, K.J., Castaglione, S., Winfield, M.O., Sala, F., Van De Wiel, C., Biedemeyer, G., Vosman, B., Matthes, M; Daly, A.; Brettschneider, R., Bettini, P. Buiatti, M.; Maestri, E., Malcevschi, A. Marmiroli, N., Aert, R., Volckaert, G., Rueda, J., Linacero, R., Vazquez, A. & Karp, A (1997) Reproducibility testing of RAPD, AFLP & SSR markers in plants by a network of European Laboratories. Mol. Breeding 3: 381-390.
Karp, A., Edwards, K.J., Bruford, M.. Funk, S., Vosman, B., Morgante, M., Seberg, O., Kremer, A., Boursot, P., Arctander, P., Tautz, D. & Hewitt, G.M. (1997) Newer technologies for biodiversity evaluation. Opportunities and challenges. Nature Biotech.15: 625-628.
Winfield, M.O., Arnold, G.M., Cooper, F., Le Ray, M., White, J., Karp, A. & Edwards, K.J. (1997) A study of genetic diversity in Populus nigra betulifolia in the Upper Severn using AFLP. Mol. Ecol. 7: 3-10.
Batley, J., Edwards, K.J., Wiltshire, C.W., Glen D.M. & Karp, A. (1998) The isolation and characterisation of microsatellite loci in the willow beetles, Phyllodecta vulgatissima (L.) and P. vitellinae (L.). Mol. Ecol. 7: 1434 -1436.
Owen, P.G., Pei, M., Karp, A., Royle, D.J. & Edwards, K.J. (1998) The isolation and characterisation of microsatellite loci in the wheat pathogen Mycosphaerella graminicola. Mol. Ecol. 7: 1611-1612.
Barker, J.H.A., Matthes, M., Arnold, G.M., Edwards, K.J. Åhamn, I., Larsson, S. & Karp, A. (1999) Characterisation of genetic diversity in potential biomass willows (Salix spp) by RAPD and AFLP analyses. Genome 42: 173-183.
Rivera, R., Edwards, K.J., Aronld, G.M., Ayad, W.G., Hodgkin, T. & Karp, A (1999) Isolation and characterisation of polymorphic microsatellites in Cocos nucifera L. Genome 42: 668-675.
Teulat, B., Trehin, R., Aldam, C., Barker, J.H.A. Arnold, G.M., Karp, A., Baudoin L. & Rognon, F. (1999) An analysis of genetic diversity in coconut (Cocos nucifera) populations from across the geographic range using sequence-tagged microsatellites (SSRs) and AFLPs. Theor. Appl. Genet. 100: 764-771.
Green, J., Edwards, K.J., Usher, S.L., Barker, J.H.A., Marshall, E.P.J., Froud-Williams, R.J. & Karp, A. (2000) Microsatellite markers for the inbreeding grass weed Barren Brome (Anisantha sterilis). Mol. Ecol. 9: 2155-2234.
Brookes, R.C., Barker, J.H.A., Bohan, D.A., Glen, D.M. & Karp, A. (2001) Microsatellites for the slugs Deroceras reticulatum and Arion intermedius. Mol. Ecol. Notes 1: 300-302.
Green, J., Barker, J.H.A., Marshall, E.P.J. Froud Williams, R.J. Peters, N.C.B. Arnold, G.M. Dawson, K. & Karp, A. (2001) Microsatellite analysis of the inbreeding grass weed Barren Brome (Anisantha sterilis) reveals genetic diversity at the within- and between-farm scales. Mol. Ecol. 10: 1035-1045.
Green, J. M., Batley, J., Peacock, L., Carter, P., Glen, D.M. & Karp, A. (2001) The molecular ecology of willow beetles (Phyllodecta spp.). Antennae 25: 254-257.
Matthes, M., Singh, R., Cheah, S-C. & Karp, A. (2001) Variation in oil palm (Eleais guineensis Jacq.) Tissue culture-derived regenerants revealed by AFLPs with methylation-sensitive enzymes. Theor. Appl. Genet. 102: 971-979.
Danquah, E.Y., Hanley, S.J., Brookes, R.C., Aldam, C. & Karp, A. (2002) Isolation and characterisation of microsatellites in Echinochloa (L.) Beauv spp. Mol. Ecol. Notes 2: 54-56.
Danquah, E.Y., Johnson, D.E., Riches, C., Arnold, G.M. & Karp, A. (2002) AFLPs and microsatellites reveal genetic diversity in Echinochloa (L). Beauv. spp. collected from different geographic origins and within rice fields at one locality. Weed Research 42, 394-405.
Gilbert (nee Stoker) K.G., Garton, S., Karam, M.A., Arnold, G.M., Karp, A., Edwards, K.J., Cooke D.T.& Barker J.H.A. (2002) A High Degree of Genetic Diversity is Revealed in Isatis spp. (dyers woad) by Amplified Fragment Length Polymorphism (AFLP) Theor. Appl. Genet. 104: 1150-1156.
Hanley, S.J Barker J.H.A., Aldam, C., Harris, S., Åhman, I., Larsson, S. & Karp, A. (2002) A genetic linkage map of willow (Salix viminalis x S. viminalis) based on AFLP and microsatellite markers. Theor. Appl. Genet. 105: 1087-1096.
Barker J.H.A., Pahlich, A., Trybush, S., Edwards, K.J. & Karp, A. (2003) Microsatellite markers for diverse Salix species. Molecular Ecology Notes 3: 4-6.
Peacock, L., Carter, P. & Karp, A. (2003) Geographic variation in phenotypic traits in Phratora spp. and the effects of conditioning on feeding preference. Entomologia Experimentalis et Applicata, 109: 31-37.
Robinson, K.M., Karp, A. & Taylor, G. (2003) Defining leaf and canopy traits linked to high yield in short rotation coppice willow. Biomass and Bioenergy. 26: 417-431.
Batley, J., Edwards K.J., Barker, J.H.A., Dawson, K., Wiltshire, C.W., Glen, D.M. & Karp, A. (2004) Molecular genetics of the beetle pests Phyllodecta vulgatissima and P. vitellinae on UK willow plantations. Insect Molecular Biology, 13: 413-421.
Peacock, L., Batley, J., Dungait, J., Barker, J.H.A., Powers, S. & Karp, A. (2004) A comparative study of interspecies mating of Phratora vulgatissima and P. vitellinae using behavioural tests and molecular markers. Ecological Entomology, 110 (3): 231-241.
Clark, J.S.C., Dani, M., Halford, N.G. & Karp A. (2005) Evidence of diversity within the SnRK1b gene family of Hordeum species. Genome 48: 1-13.
White, G.M., Moss, S.R. & Karp, A. (2005) The molecular basis of resistance to the cyclohexanedione herbicide sethoxydim in Lolium multiflorum Lam. Weed Research
Hanley, S.J., Mallott, M.D. & Karp A (2006) Alignment of a Salix linkage map to the Populus genomic sequence reveals macrosynteny between willow and poplar genomes. Tree Genetics and Genomes 3: 35-48.
Trybush, S., Kang-Hyun, C., Hanley, S.J., Jahodová, S., Grimmer, M., Bayon, C., Emelianov, I. &, Karp, A. (2006) Getting the most out of fluorescent AFLPs. Can. J. Bot. 84 (8): 1347-354.
Grimmer, M.K., Trybush, S., Hanley, S., Karp, A. & Asher M.J.C. (2007) A paternal anchored linkage map for sugar beet and mapping of a new source of resistance to beet necrotic yellow vein virus. Theor. Appl Genet 114: 1151-1160.
Jahodová, S., Trybush, S., Pysek, P., Wade, M. & Karp A. (2007) Amplified fragment length polymorphism reveals three tall invasive species of Heracleum in Europe. J. Biogeog. Div. Distr. (2007) 13: 99-114.
Kuzovkina, Y.A., Weih, M., Abalos Romero, M., Charles. J., Hurst, S., McIvor, I., Karp, A., Trybush, S., Labrecque, M., Teodorescu, T.I., Singh, N.B., Smart, L.B. & Volk, T.A. (2008) Salix: Botany and Global Horticulture. Horticultural Reviews 34: 447-489.
Trybush, S; Jahodova, S; Macalpine, W; Karp A (2008) Genetic studies of a germplasm resource reveal new insights into relationships among Salix subgenera, sections and species. BioEnergy Research 1: 67-79
Karp, A; Shield, I. (2008) Bioenergy from plants and the sustainable yield challenge. New Phytologist Tansley Review 179: 15-32
Shield, I; Macalpine W, Karp A. (2008) The effect of the size of the cuttings planted on the subsequent performance of 3 contrasting willow cultivars for short rotation coppice. Aspects of Applied Biology 90: 225-231
Macalpine, W; Shield, I; Trybush, S; Hayes C and Karp, A. (2008) Overcoming barriers to crossing in willow (Salix spp.) breeding. Aspects of Applied Biology 90: 173-180
Haughton, A.J. ; Bond A.J., Lovett A.A.; Dockerty T.; Sünnenberg G, Clark S.J., Bohan D.A, Sage, R.B., Mallott M.D., Mallott V.E., Cunningham M.D, Riche A.B., Shield I.F.; Finch, J.W.; Turner, M.M.; Karp, A. (2009). A novel, integrated approach to assessing social, economic and environmental implications of changing rural land-use: a case study of perennial biomass crops. Journal of Applied Ecology 46: 323-333
Lovett AA; Sünnenberg GM; Richter GM; Dailey AG; Riche AB and Karp A. (2009) Land use implications of increased biomass production identified by GIS-based suitability and yield mapping for Miscanthus in England. Bioenergy Research 2: 17-28
Bayon, C.; Pei, M; Ruiz, C; Hunter, T; Karp, A; Tubby, I (2010) Genetic structure and population dynamics of a heteroecious plant pathogen Melampsora larici-epitea in short-rotation coppice willow plantations". Molecular Ecology 18: 3006-3019.
King RA, Harris SL, Karp A and Barker JHA (2010) Characterisation and inheritance of nuclear microsatellite loci for use in population studies of the allotetraploid Salix alba-S. fragilis complex. Tree Genetics and Genomes 6: 247-258
Brereton, N.J., Pitre, F.E. Hanley, S.J., Ray, M., Karp, A. and Murphy, R.J. (2010) Mapping of Enzymatic Saccharification in Short Rotation Coppice Willow and Its Independence from Biomass Yield. Bioenergy Research 3: 251-261
Sage, R., Cunningham, M., Mallott, M., Haughton, A., Bohan, D., Riche, A., Karp, (2010) A. Birds in Miscanthus fields in SW England in summer and winter compared to arable grass and short rotation coppice willow and effects of crop structure. Ibis 152: 487-499
Pitre FE, Brereton, NJB, Audoire S, Richter GM, Shield I, Karp A (2010) Estimating root biomass in Salix viminalis x Salix schwerinii cultivar "Olof" using the electrical capacitance method. Plant Biosystems 144: 479-483
Pei, M.H., Ruiz, C., Shield, I., Macalpine, W., Lindegaard, K. Karp, A. (2010) Mendelian inheritance of rust resistance in hybrids between Salix sachalinensis and S. viminalis to Melampsora larici-epitea. Plant Pathology 59, 862–872
Bond, A, T Dockerty, A Lovett, A B Riche, A J Haughton, D A Bohan, R B Sage, I F Shield, J W Finch, M M Turner, Karp A (2010), "Learning how to deal with values, frames and governance in Sustainability Appraisal" Regional Studies. DOI: 10.1080/00343404.2010.485181
Karp, A. Hanley, S.J., Trybush, S.O.T., Macalpine, W., Pei, M., Shield I. (2011) Genetic improvement of willow for bioenergy and biofuels J. Integrative Plant Biology 53 (2) 151-165
Hanley S.J., Pei, M.,Powers, S.J., Ruiz, C., Mallott, M.D., Barker, J.H.A., Karp, A. (2011) Genetic mapping of rust resistance loci in biomass willow Tree Genetics and Genomes DOI: 10.1007/s11295-010-0359-x
Karp, A., Richter, G.M. (2011) Meeting the challenge of food and energy security. J.Experimental Botany.62: 3263-3271
Brereton, N.J.B., Pitre, F.E., Ray, M.J., Karp, A and Murphy, R.J. (2011) Investigation of Tension Wood Formation and 2, 6-dichlorbenzonitrile Application in SRC Willow Composition and Enzymatic Saccharification. Biotechnology for Biofuels 4: 13. doi: 10.1186/1754-6834-4-13
Aradottir,G.I.. Hanley, S.J., Collins, C.M., Dawson, K.J., Karp, A., Leather, S.R., Shield, I., Harrington, R. (2011) Population genetics of Tuberolachnus salignus, an obligate parthenogenetic aphid. Agricultural & Forest Entomology (In Press).
Jones, R.N. & Karp, A. (1986) ‘Introducing Genetics’ John Murray. London.
Karp, A., Isaac, P.G. & Ingram, D.S. (1998) ‘Molecular Tools for Screening Biodiversity: Plants and Animals’ Chapman & Hall, London.
Jones R.N., Karp A. & Giddings D.G. (2001) ‘The Essentials of Genetics’ John Murray. London
Halford N, G. & Karp, A. Energy Crops. RSC Energy and Environment Series. RSC Publishing.
c1) Invited reviews \chapters in books
Karp, A. & Bright, S.W.J. (1985) On the causes and origins of somaclonal variation. In: Oxford Surveys of Plant Molecular and Cell Biology. Vol. 2. (ed, B.J. Miflin) pp 199-234.
Bright, S.W.J., Ooms, G., Foulger, D., Karp. & Evans, N. (1986) Mutation and tissue culture. In: Plant Tissue Culture and it's Agricultural Applications (eds, L.A. Withers, P.G. Anderson) Butterworths, Kent pp 431-449.
Jones, M.G.K., Karp, A. (1986) Plant tissue culture technology and crop improvement. In: Advances in Biotechnological processes. Vol. 5, pp 91- 121.
Karp, A., Jones, M.G.K., Ooms, G. & Bricht, S.W.J. (1986) Potato protoplasts and tissue culture in crop improvement. In: Biotechnology & Genetic Engineering Reviews. Vol. 5 (ed, G.E. Russell) Intercept pp 1-32.
Karp, A. (1989) Can genetic instability be controlled in plant tissue cultures?. IAPTC Newsletter. No. 58, pp 2-11.
Karp, A. (1990) Somaclonal variation in potato. In: Biotechnology in Agriculture and Forestry (ed, Y.P.S. Bajaj). Sringer-Verlag. Berlin pp 379-399.
Karp, A., Wu, W.S., Maddock, S.E. & Jones, M.G.K. (1990) Chromosome instability in bread-wheat (Triticum aestivum) cell suspensions and their dividing protoplasts. In: Biotechnology in Agriculture and Forestry (ed, Y.P.S. Bajaj) Springer-Verlag, Berlin pp 481-493.
Karp, A. (1991) Cytological techniques. In: Plant Tissue Culture Manual. (ed, K. Lindsey) Kluwer Academic Press pp 1-13.
Karp, A. (1991) On the current understanding of somaclonal variation. In: Oxford Surveys of Plant Molecular and Cell Biology (ed., B.J. Miflin) Oxford University Press. Vol. 7, pp 1-58.
Karp, A. & Lazzeri, P.A. (1991) Regeneration, stability and transformation of barley. In: Barley: Genetics, Biochemistry, molecular Biology and Biotechnology (ed, P.R Shewry) CAB International Press pp 549-571.
Karp, A. (1992) The role of growth regulators in somaclonal variation. British Society for Plant Growth Regulation. Annual Bulletin No 2. May 1992 pp 1-9.
Karp, A. (1993) Labelling of double stranded probes with biotin. Humana Press, Totowa, New Jersey (ed. P.J. Isaac) pp 149-152.
Karp, A. (1993) Preparation of chromosome spreads by root-tip meristem dissection for in situ hybridisation with biotin-labelled probes. Humana Press, Totowa, New Jersey (ed P.J. Isaac) pp 83-88.
Karp, A. (1993) Use of biotin labelled probes on plant chromosomes. Humana Press, Totowa, New Jersey (ed., P.J. Isaac) pp 161-166.
Karp, A. (1995) Somaclonal variation as a tool for crop improvement. In: The Methodology of Plant Genetic Manipulation: Criteria for Decision Making. (eds. Cassels, A.C.; Jones, P.W.) Kluwer Academic Publishers (Dordrecht/ Boston/London).
Karp, A. & Edwards, K.J. (1997) DNA Markers - A Global Overview. In: DNA Markers: Protocols, Applications and Overviews. (eds Caetano-Anolles G., Gresshoff PM.) J. Wiley and Sons. NY. USA pp 1-13.
Karp, A., Kresovich, S., Bhat, K.V., Ayad, W.G. & Hodgkin, T. (1997) Molecular Tools in plant genetic resources conservation: a guide to the technologies. IPGRI Technical Bulletin No.2.
Karp, A. & Peacock, L. (2004) The ecology and population genetics of the blue and brassy willow beetles (Phyllodecta (= Phratora) vulgatissima L.) and P. vitellinae on United Kingdom willow (Salix) plantations. In: New Developments in the Biology of Chrysomelidae pp 97-104.
Karp A, Haughton AJ, Bohan DA, Lovett A; Bond AJ, Dockerty T, Sünnenberg G, Finch JW, Sage RB, Appleton KJ, Riche AB, Mallott MD, Mallott VE, Cunningham MD, Clark S and Turner MM. Chapter 4: Perennial Energy Crops: Implications and Potential. In : What is Land For? The Food, Fuel and Climate Change Debate (Ed Michael Winter and Matt Lobley) Earthscan, London UK
Karp A, Halrford N.G. Energy Crops: Introduction. In Energy Crops. (Halford N, G. & Karp, A eds)/ RSC Energy and Environment Series. RSC Publishing
c2) Chapters in conference proceedings
Bright, S.W.J., Nelson, R.S., Karp, A., Jarrett, V.A., Creissen, G.P. Ooms, G., Miflin, B.J. & Thomas, E. (1982) Variation in culture-derived potato plants. In: Proc. 5th Intl. Cong. Plant Tissue and Cell Cultures. Tokyo pp 413-414.
Jones, M.G.K., Maddock, S.E., Karp, A., Nelson, R.S. Creissen, G.P. Foulger, D. & Bright, S.W.J. (1984) Tissue and protoplast culture- a novel way to new crop varieties. In: The World Biotechnology Report 1984 Vol. 1. Europe Online Publications Ltd. pp 443-454.
Bright, S.W.J., Ooms, G., Karp, A., Foulger, D., Fish, N., Jones, M.G.K. & Evans, N.E. (1985) In: Genetic Manipulation in Crop Plants (eds, M.W. Swaminathan, H. Hu, Q.Q. Shaoi). Chinese Agriculture and Science Technology Press. Beijing. pp781-881.
Famaeler, Y., Cammaerts, D., Karp, A., Sidorov, V., De-Brouwer, D., Negrutiu, I. & Jacobs, M. (1986) Cellular engineering by gamma fusion and egg transformation. Recent Experimental data and applications in plant breeding. In: Nuclear Techniques and in vitro culture for plant improvement. IAEA Vienna. pp 453-461.
Famaeler, Y., Karp, A., Sidorov, V., Negrutiu, I., Cammaerts, D. & Jacobs, M. (1986) Cellular engineering by means of gamma fusion: Genetic variation in fusion products. In: Somaclonal Variation in Crop Improvement (ed, J. Semal) Gembloux. pp 260-263.
Karp, A. (1986) Chromosome variation in plants regenerated from protoplasts and cultured plant tissues. In: Somaclonal Variation in Crop Improvement (ed, J. Semal) Gembloux. pp 28-35.
Karp, A. (1986) Chromosome variation in regenerated plants. In: Genetic Manipulation in Plant Breeding (eds, W. Horn, C.J. Jensen, W. Odenbach, O. Schieder. Walter de Gruyter & Co., Berlin. pp 547-554.
Ooms, G., Burrell, M.M., Karp, A., Twell, D. & Roberts, J. (1986) Genetic manipulation in potato. In: Genetic Manipulation in Plant Breeding. (Eds, W.H. Horn, C.J. Jensen, W. Odenbach & O. Schieder. Walter de Gruyter & Co. Berlin pp 823-825.
Karp, A. & Shewry, P.R. (1987) Cell and molecular biology: What do they have in store for the grain trade? In: Proc. Int. Grain Forum. Amsterdam. pp 129-148.
Jones, M.G.K., Karp, A., Jones, H., Cooper-Bland, S., Lindsey, K., Wu, Q.S. Lee, B.T. de Both, M.T. J. & Fish, N. (1987) Electrofusion and electroporation of crop plant protoplasts. In: Genetic and Cellular Engineering of Plants and Microorganisms important in Agriculture. Abstracts CEC Meeting of Biotechnology Action Programme, Louvain, 1987. Eds D. de Nettancourt & E. Magnien. Louvain : CEC pp 97-98.
Karp, A. (1988) The origins and causes of chromosome instability in plant tissue culture and regeneration. In: Kew Chromosome Conference III (ed, PE Brandham). HMSO, London pp 185-192.
Bebeli, P., Karp, A. & Kaltsikes, P.J. (1989) Morphogenetic response of isogenic lines of Triticale differing in their content of hetero-chromatin. In: Proc. 7th Int. Wheat Symp. Vol. 1 (eds T.E. Miller, R.M.D. Koebner) IPRS Cambridge pp 711-714.
Bebeli, P., Karp, A. & Kaltsikes, P.J. (1989) Morphogenetic response of sister lines of rye differing in their content of heterochromatin. In Proc. 7th Int. Wheat Genetics Symp. Vol. 1 (eds, T.E. Miller, R.M.D. Koebner) IPRS Cambridge pp 715-718.
Jones, M.J.K., Duncley, R., Steele, S.H., Karp, A., Gibson, R., Fish, N., Valkonen, J., Poutala, T. & Pehu, E. (1990) Transfer of resistance to PLRV, PVX and PVY from S. brevidens to potato by somatic hybridisation: Characterisation and field evaluation. In; Progress in Plant Cellular and Molecular Biology (eds, H.J.J. Nijkamp, L.H.W. Van der Plas, J. Van Aartrijk). Kluwer Academic Publishers, Dordrecht, Boston, London pp 286-292.
Gibson, R.W., Valkonen, J., Pehu, E., Jones, M.G.K. & Karp, A. (1992) Broad spectrum virus resistance in Solanum brevidens and its introduction by protoplast fusion to cultivated potato. In: Biotechnology: Enhancing Research on Tropical Crops in Africa. (eds, Thottappilly, G.,Monti, L.M., Mohan Raj, D.R., Moore, A.W.) CTA, IITA 305-306.
Karp, A. & Edwards, K.J. (1996) Molecular techniques and Biodiversity Evaluation. In: Proceedings of the National Congress of Genetics, Kuala Lumpar. Malaysia November 13-15th.
Karp, A. & Edwards, K.J. (1996) Molecular techniques in the analysis of the extent and distribution of diversity. In: Molecular genetic techniques for plant genetic resources. Report of an IPGRI workshop, 9-11 October, 1995, Rome, Italy.
Karp, A (1999) Biodiversity in Agricultural systems: New challenges for genome diversity studies. In Genomes: Proceedings of the 22nd Stadler Genetics Symposium (ed. JP Gustafson) Plenum Press, New York, USA pp 99-108.
Karp, A. (1999) The use of polymorphic microsatellites for assessing genetic diversity in coconut. In: Current Advances in Coconut Biotechnology. Kluwer. Academic Press. (Dordrecht) pp 121-130.
Karp, A. (2002) The New Genetic Era: Will it help us in managing plant genetic diversity? In: Managing Plant Genetic Diversity (ed Engels JMM, Ramanatha Rao V, Brown AHD, Jackson MT). CABI pp 43-56.
d) Other publications
d1) Abstracts in conference proceedings
Karp, A. (1982) Chromosome variation in regenerated potato plants. In: Kew Chromosome Conference II. (eds, P.E. Brandham; Bennet, M.D.) George Allen & Unwin Ltd. pp 351.
Jones, M.G.K., Bright, S.W.J., Nelson, R.S., Foulger, D., Creissen, G.P., Karp, A. & Ooms, G. (1983) Variation in plants regenerated from protoplasts and complex explants of potato. Experentia Supplementum 45: 150-151.
Jones, R.N. & Karp, A. (1983) Genetic control of chiasma distribution of Lolium perenne. In: Proc. IVth International Congress of Genetics, New Delhi. pp 665.
Nelson, R.S. Karp, A., Creissen, G.P. & Bright S.W.J. (1983) Plants regenerated from isolated protoplasts of Solanum brevidens. Experientia Supplementum 45: 68-69.
Bright, S.W.J., Foulger, D., Evans, N.E., Karp, A., Nelson, R.S., Creissen G.P. & Jones, M.G.K. (1984) Potato plants regenerated from protoplasts and explants: nature and application of variation. In: The Genetic Manipulation of plants and it's application to agriculture (eds, P.J. Lea, G.R. Stewart, Oxford. Oxford University Press. pp 265.
Bright, S.W.J., Jones, M.G.K., Ooms, G., Maddock, S., Karp, A., Foulger, D. & Miflin, B.J. (1986) Biotechnology's potential for crop improvement. In: Biotechnology's potential for increasing crop production efficiency. National Agricultural Conference, Stoneleigh.
Foulger, D., Fish, N., Karp, A., Bains, A., Cooper-Bland, S., Bright, S.W.J. & Jones, M.G.K. (1986) Protoplast fusion of Solanum species. In : Proceedings 6th International Congress Plant Tissue Culture, Minneapolis, 1986. Eds D.A. Somers et al. Minneapolis: University of Minnesota\International Association for Plant Tissue Culture. p 420.
Lee, B.T. Murdoch, K., Topping, J., de Both, M.T. J., Wu, Q.S. Karp, A., Steele, Symonds, C., Kreis, M. & Jones, M.G.K. (1987) Isolation, culture and morphogenesis from wheat protoplasts and study of expression of DNA constructs by direct gene transfer. In: Proceedings 7th International Protoplast Symposium, Wageningen, 1987 p 24.
Ooms, G., Risiott, R., Karp, A. & Phelpstead, J. (1988) Transformation in potato. Abstracts 2nd International Congress Plant Molecular Biology, Jerusalem, p 588.
Pehu, E., Clark, M., Karp, A. & Jones, M.G.K. (1988) Molecular characterisation of somatic hybrids of Solanum tuberosum and S. brevidens. Abstracts 2nd International Congress Plant Molecular Biology, Jerusalem, p 432.
Pehu, E., Karp, A. & Jones, M.G.K. (1988) Chromosome number and parental nuclear and chloroplast DNA contributions in somatic hybrids of dihaploid S. tuberosum and S. brevidens. Abstracts Eucarpia Meeting on Genetic Manipulation in Plant Breeding, Elsinore, Denmark, p 111.
Pehu, E., Potter, R., Karp, A. & Jones, M.G.K. (1988) Variation in chromosome number in somatic hybrids of diploid potato (Solanum tuberosum and S. brevidens). Proceedings 15th congress of the Scandinavian Society of Plant Physiology, Turku, Finland, p 22A.
Jones, M.G.K., Dunckley, R., Steele, S., Pehu, E., Karp, A. & Gibson, R.W. (1989) Improving potatoes. Proceedings of the International Biological Conference Leicester, 1989 p 7.
Pehu, E., Gibson, R.W. Karp, A. &, Jones, M.G.K (1989) Cytogenetical analysis of parental genome instabilities in somatic hybrids of Solanum tuberosum and S. brevidens using species-specific probes. In: Proceedings of the 1st International Symposium on the molecular Biology of the potato, Maine, Abstract p 38.
Pehu, E., Karp, A., Gibson, R.W. Thomas, M., Malone, R. & Jones, M.G.K. (1989) Identification and characterisation of Solanum brevidens and S. tuberosum specific DNA sequences and their use in a somatic hybridisation programme. In: Proceedings of the 1st International Symposium on the Molecular Biology of the Potato. Maine. Abstract p 67.
Jones, M.G.K., Jones, H., Dunckley, R., Karp, A. & Pehu, E. (1990) The application of protoplast technology to potato improvement. In: European Association of Potato Research Meeting, Edinburgh, 1990. Abstract 96.
Pehu, E., Karp, A., Thomas, M., Clark, M. & Jones, M.G.K. (1990) Cytogenetical analysis of parental genome stabilities in somatic hybrids of Solanum tuberosum and S. brevidens using species-specific probes. Proceedings of the 10th International Chromosome Symposium, Uppsala 1989, Abstract p 103.
Pehu, E., Malone, R., Karp, A. & Jones, M.G.K. (1990) Application of linkage group specific RFLPs to study assymetric hybrids of S. tuberosum and S. brevidens. In: European Association of Potato Research meeting, Edinburgh, Abstract p 13.
Cannell, M., Karp, A., Shewry, P.R. & Isaac, P. (1991) Restriction fragment length polymorphism analysis in barley and assignment of characterised probes to chromosome arms. In: Barley Genetics VI. Proc 6th Intl. Barley Genet Symp. (Helsingborg, Sweden).
Bebeli, P.J., Kaltsikes, P.J. & Karp, A. (1992) Somaclonal variation for agronomic traits in near isogenic lines of triticale differing in telomeric heterochromatin. Proc. Symp. New Genetical Approaches to Crop Improvement. Karachi.
Bebeli, P.J., Karp, A. & Kaltsikes, P.J. (1992) Somaclonal variation in sister lines of rye differing in telomeric heterochromatin. Proc. Symp. New Genetical Approaches to Crop Improvement. Karachi.
Barker, J.H.A., Edwards, K.J. & Karp, A. (1996) Molecular techniques and genetic diversity in willow. Conference abstract from the 16th annual meeting for the Tree Biotechnology Group, University of Bath, April 1996.
Barker, J.H.A., Pahlich, A., Edwards, K.J. & Karp, A. (1997) Studies on the genetic diversity of Salix spp. Journal of Experimental Botany, 48, Supplement, SEB Meeting, Book of Abstracts, p 93.
Beismann, H., Barker, J.H.A., Karp, A. & Speck, T. (1997) Genetic variation of three Salix taxa measured with AFLP technique along an ecological gradient and the effect of their biomechanical properties on dispersal. Conference abstract from the 10th meeting on Plant Population Biology of the GFO (Gesellschaft fur Okologie), University of Zurich, May 1997.
Beismann, H., Barker, J.H.A., Karp, A. & Speck, T. (1997) Genetic variation of three Salix taxa measured with AFLP technique along an ecological gradient and the effect of their biomechanical properties on dispersal. Journal of Experimental Botany, 48, Supplement, SEB Meeting, Book of Abstracts, p 91.
Barker, J.H.A., Lindegaard, K.N., Arnold, G.M., Royle, D.J. & Karp, A. (1998) Characterisation of genetic diversity in biomass willows (Salix spp) using molecular markers. Conference abstract from the 18th annual meeting for the Tree Biotechnology Group, East Malling, April 1998.
Arnold, G.M., Barker, J.H.A. & Karp, A. (1999) Some approaches to the analysis of microsatellite (SSR) data. Abstract from the 11th International Statistical Conference of Genstat Users, Poznan, Poland, 18â€‘21 May 1999.
Batley, J., Barker, J.H.A., Edwards, K.J., Glen, D.M., Wiltshire, C.W. & Karp, A. (1999) A Study of the Population Ecology of Willow Beetles using Microsatellites. Abstract from EURECO ’99 – 8th European Ecological Congress, Halkidiki, Greece, 18-23 September 1999.
Lindegaard, K.N., Hanley, S.J., Barker, J.H.A. & Karp, A. (1999) Genetic improvement of willows for renewable energy and fibre. Poster abstract from the International Poplar Symposium II, Orleans, France 13-17 September 1999.
Barker, J.H.A., Teulat, B., Lebrun, P. Baudouin. L., Karp, A. & Rognon, F. (2000) Coconut microsatellite markers and their use in genebank organisation and genetic mapping. Abstract from the International Plant & Animal Genome Conference VIII, San Diego, California,USA, January 2000.
Hanley, S.J., Barker, J.H.A., Aldam, C., Lindegaard, K.N., Pei, M.H., Hunter, T. & Karp A. (2000) Improving breeding efficiency of biomass willows using molecular marker technology. Abstract from the International Plant & Animal Genome Conference VIII, San Diego, California, USA, January 2000.
Riche, A., Karp, A., Pei, M., Shield, I. & Yates, N. (2007) Grass and woody biomass species – agronomic requirements learnt from 15 years field experimentation. International Plant Protection Congress, Glasgow, UK 15-18 Oct 2007.
Richter, G.M, Lovett, A, Sunnenberg, G., Dailey, G., Riche, A. & Karp, A. (2007) Integration of crop models and constraint mapping for the projection of bioenergy production. Farming Systems Design 2007 International symposium, Catania, Italy 10-12 Sept 2007.
d2) Technical reports (non-confidential)
Karp, A. (1992) Molecular control of genetic variability in regeneration of crop plants. In: Biotechnology Research for Innovation, Development and Growth in Europe (1990-1993) Progress Report 1992 (ed., A. Vassarotti) pp 154-148.
Karp, A. (1993) Molecular control of genetic variability in regeneration of crop plants. In: Biotechnology Research for Innovation, Development and Growth in Europe (1990-1993) Progress Report 1993 (ed., A. Vassarotti) pp 187-191.
Molecular Screening News Nos 1. August (1993) -19 December (1999).
Karp, A. (1994) Development of rapid novel molecular and cellular tools for screening and evaluation of genetic diversity. (eds Taxis du Poët, P., Vassarotti, A., Baselga, de Elorz, J Vol 1. Abstracts.
Karp, A. (1994) Development of rapid novel molecular and cellular tools for screening and evaluation of genetic diversity. (eds Taxis du Poët, P., Vassarotti, A., Baselga, de Elorz, J Vol 2. Detailed Presentations pp. 776-790.
Karp, A. (1994) Molecular control of genetic variability in regeneration of crop plants. In: Biotechnology Research for Innovation, Development and Growth in Europe (1990-1993) Progress Report 1994 (ed., A. Vassarotti).
Karp, A. (1995) Development of rapid novel molecular and cellular tools for the screening and evaluation of genetic diversity of genetic diversity in plants. European Commission Final Report. Biotechnology (ed A. Hoeveler, M. Cresti) (1992-1994) pp 557-563.
Karp, A. (1995) Molecular Genetic Screening Tools. European Commission Final Report. Biotechnology (ed A. Hoeveler, M. Cresti) (1992-1994) pp 543-550.
Ayad, G., Karp, A., Edwards, K.J. & Rivera, R (1998) Determining how molecular methods can best be used to locate maximum genetic diversity for ex situ and in situ conservation through on-site sampling analysis in coconut (Cocos nucifera). In: DFID and the Consultative Group on International Agricultural Research (CGIAR) : The Competitive Research Facility 1990-1997 (ed., E.J. Warham) pp 107-109.
› PhD Supervision: PhD Supervisor Gia Arradottir: BBSRC Quota
› Inivted Speaker; New Phytologist Bioenergy Trees conference
› European Society of Agronomy international conference (Montpellier)
› Food Security international conference (Lancaster Sept 12-14)
› Can Bio international conference and EU mission (Vancouver Sept 26-29)
› â€˘ European Science Foundation â€śBioenergyâ€ť European conference (Dublin Apr 25-27)
› SRC European conference (nr. Hamburg)
› Cheltenham Science Festival (national public conference) (UK Jun 03)
› Spaital Impacts of Biomass: Berlin
› Botanical Congres: Leipzig
› EPSO Biofuels workshop; London (Royal Scociety)
› Global Change Biology: Bioenergy Editor
› Chelsea Flower Show Gold Medal
› Alfred Toepfer prize: Alfred Toepfer prize for Agriculture, Forestry and Nature Conservation
› Editor of BioEnergy Research
› RASE Research Medal: Awarded Royal Agricultural Society of England Research medal for energy crop reserach
› PhD Supervisor Nick Brereton: PhD Supervision - Porter Studentship with Imperial College
› PhD Supervisor Rocio Alarcon-Reverte: PhD Supervision - Co-supervisor with Steve Moss (main)
› Renewables Link Committee member
› Subject Editor of Weed Research
› PhD Supervisor Ron Marshall: PhD Supervision - Co-Supervisor with Steve Moss (main)
› Biofuels, Bioproducts and Biorefining editor
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