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Research interests:

I have 13 years experience of developing and applying plant transformation technologies. I have developed and refined enabling technologies for transforming wheat and other species using Agrobacterium and particle bombardment and the regeneration of plants through somatic embryogenesis. I have interests in Cisgenesis, in validating cereal promoters for targeting expression to predefined tissues, in sequences for tagging or targeting proteins encoded by transgene and in the risk assessment of GM crops. I manage a fully functioning transformation laboratory with access to glasshouse and constant environment plant growth facilities for appropriate for GM containment. I have held Defra licences for noncommercial, environmental release of transgenic wheat modified for research purposes in the UK.

I collaborate with 'cereal trait-specialists' within the BBSRC and more widely to facilitate a transgenic approach to problem solving in wheat. I also have several technology-based research interests, some of which are outlined below.

General wheat transformation capability (See posters for further details) BRACT Poster; Agro Optimisation Poster; Current Status Poster

I lead one of the few labs with expertise in routine wheat transformation using either biolistics or Agrobacterium DNA-delivery methods as appropriate. We have experience of transforming over 30 different wheat genotypes including durum and elite varieties and possess a catalogue of previously characterised tissue-specific, inducible and constitutive promoters. We regularly use both over-expression or RNAi silencing approaches and incorporate immuno-reactive tags e.g. FLAG, c-MYC & Ha for identification, or purification of recombinant protein. Outputs include over 40 journal papers or book chapters describing candidate gene analysis or technology development since 2005. Time scales are: 12 weeks from DNA-delivery to PCR-positive plants with a further 14 weeks to T1 plants via embryo rescue or 24 weeks to T1 seed. Service transformation available at FEC costings.

Y. He, et al. (2010). Journal Experimental Botany, 61: 1567 - 1581.

H Wu, et al. (2008). Transgenic Research 17(3): 425-436.

H Wu, et al. (2006). Molecular Breeding, 18: 195-208.

HD Jones, et al. (2005). Plant Methods 1: 5.

HD Jones (2005). Journal of Cereal Science. Vol 41 (2): 137-147

H Wu, et al. (2003). Plant Cell Reports 21: 659-668.

Targeting GFP to wheat plastids (See poster for further details)

The ability to target marker proteins to specific subcellular compartments is a powerful research tool to study the structure and development of organelles. Here transit sequences from nuclear-encoded, plastid proteins, namely rice FtsZ, maize non-photosynthetic ferredoxin III (FdIII) and the small subunit of RubisCO were used to target a modified synthetic GFP (S65G, S72A) to plastids. The localisation of the fusion proteins expressed in transgenic wheat plants and under the control of the rice actin promoter were compared to an untargeted GFP control. GFP fluorescence was localised to non-green plastids in pollen, roots and seed endosperm and detected in isolated leaf chloroplasts using a GFP-specific antibody. Transit peptides appeared to influence the relative fluorescence intensities of plastids in different tissues. This is consistent with differential targeting and/or turnover of GFP fusion proteins in different plastid types. Replacement of GFP sequences with alternative coding regions enables immediate applications of our vectors for academic research and commercial applications.

LF Primavesi, et al. (2008) Transgenic Research 17(4): 529-543.

Heat-inducible expression system for wheat transgenics (See poster for further details).

Constitutive promoters are widely used to functionally characterise plant genes in transgenic plants but their lack of specificity and control over protein expression can be a major disadvantage. On the other hand, promoters that provide precise regulation of temporal or spatial transgene expression facilitate such studies by targeting over-expression or knockdown of target genes to specific tissues and/or at particular developmental stages. Here we used the uidA (GUS) reporter gene to demonstrate that the barley Hvhsp17 gene promoter can be induced by heat treatment of 38-40 °C for 1-2 hours in transgenic wheat. The GUS enzyme was expressed only in those tissues directly exposed to heat and not in neighbouring tissues. The induction of HSP: : GUS was demonstrated in all organs and tissues tested but expression in older tissues was lower. Proximal root sections showed less GUS activity than in root tips. This heat-inducible promoter provides the ability to investigate the function of candidate genes by up- or down-regulation of expression in selected tissues or developmental stages of a transgenic plant, limited only by the ability to apply a heat shock to the selected tissues. It also allows the investigation of genes that would be lethal or reduce fertility if expressed constitutively.

J. Freeman, et al. (2011). Plant Biotechnology Journal On Line DOI: 10.1111/j.1467-7652.2011.00588.x

Rapid-cycling Space wheat (See poster for further details)

Conventional wheat varieties have long life-cycle of approximately 6 months from seed to seed. This is a problem for projects using wheat transgenics where homozygous lines need to be identified, when crossing is required, or when large quantities of seeds from specific lines needs to be bulked. A rapid-cycling, dwarf wheat cultivar (USU-Apogee) was released by the Utah Ag. Exp. Sta. in cooperation with the National Aeronautics and Space Administration (NASA) in April 1996. This cultivar was developed to provide improved food crops for bio-regenerative life support systems in space (space farms). USU-Apogee is a full dwarf (45 cm tall), hard red spring wheat developed for high yields in controlled environments. Under continuous light, time to heading is only 25 days and 60 to 65 days to harvest. Under our standard diurnal growth room conditions (16h light, temp 18C day / 15C night) time to heading is 35 days and seeds can be harvested at 80 to 85 days which represents approximately half the life-cycle duration of our current model transformation genotype Cadenza.

Promoters for wheat transformation (See poster for further details).

One of the current limitations in functional genomics using wheat transformation is the lack of suitable promoters to drive the candidate genes cassettes. Our aim is to develop a catalogue of well-characterised promoters with known ‘strengths’ and expression profiles. We have used the UidA reporter gene encoding the GUS enzyme in transgenic wheat plants to analyse a range of defined promoter sequences.

Chromosomal location of transgene insertions revealed by FISH (See poster for further details).

The transformation of wheat, predominantly by biolistic methods, has recently become routine in many laboratories. Little is known of the relationship between physical location of transgene and their integration, expression and inheritance. The integration process itself is poorly understood but thought to occur via illegitimate recombination and to be essentially random. We used fluorescent in-situ hybridisation techniques to investigate whether there was bias in the integration patterns either within or between wheat chromosomes or genomes.

Wheat chloroplast transformation studentship

Chloroplasts are the site of photosynthesis and many other fundamental metabolic reactions. They contain their own set of genes and an expression apparatus to transcribe and translate these genes into functional proteins. There are several important targets including reducing photorespiration by manipulating the kinetics of rubisco that could be pursued if it were possible to engineer wheat plastid genes. In addition, transformation of the plant chloroplast genome has many compelling trait-independent features including: precise targeted integration of trait genes via homologous recombination; the ability to accumulate proteins to extremely high levels and maternal inheritance of chloroplast genes which prevents the pollen-mediated spread of transgenes. However, chloroplast transformation in cereals has not yet been achieved and is hampered by a lack of applied research into the morphology and developmental state of plastids able to integrate exogenous DNA, the uncertainty over appropriate tissue targets for DNA-delivery and the absence of methods to identify and select rare heteroplasmic events and drive the conversion to homoplasmy. This studentship will identify the key limiting steps in DNA-delivery/homologous recombination in the wheat plastid genome and in the regeneration/selection of homoplasmic cells. The ultimate goal is to understand and overcome the bottlenecks currently limiting plastid transformation in wheat.

Zinc-finger nucleases for targeted mutation in wheat

In collaboration with Dow AgroSciences, we have begun to evaluate the use of zinc-finer nucleases to generate site-specific mutations in the wheat genome.

Project Leader

Member

Project Leader

  Analysis of cereal transplastomic technology for preventing the expression and spread of transgenes in pollen

To assess gene flow through and gene expression in plastids present in cereals and grass pollen. We will generate stable nuclear transgenic wheat containing organelle targeted GFP to monitor the fate of GFP-tagged organelles during pollen development. The presence, integrity and expression of plastid DNA in pollen from barley, Lolium, maize, Secale and wheat will be determined. Identification of inter- and intra-specific plasmid DNA polymorphisms will be used to assess the frequency of transmission of paternal plastids in inter- and intra-specific crosses involving barley, Lolium, maize, Secale and wheat. Crop to crop and crop to weed transmissions of plastid DNA will be examined by sampling experimental plots and weed populations.

  Biotechnology resources for arable crop transformation (BRACT)

The BRACT research programme aims to provide an efficient transformation capability for the main UK crops, while facilitating further improvements in transformation technology and delivery to the UK research community. It is a collaboration between Rothamsted Research and the John Innes Centre. The work at Rothamsted Research will focus on developing protocols for Agrobacterium-mediated wheat transformation, testing constructs in wheat and on developing ‘clean gene’ transformation methods for wheat.
The key arable crops included in this proposal are wheat, barley and oilseed rape while others are included as secondary crops or addressed in separate proposals from other groups. For the three major crops, the programme will provide all the resources needed to facilitate transformation. These will include constructs requiring the minimum effort to insert genes or promoters of interest, through to efficient transformation methodology that builds in the capacity to produce transgenic plants free of selectable marker genes. To facilitate the production of marker free plants, Agrobacterium tumefaciens-mediated transformation will be used, although biolistic methodology will be available if required.
The principal route to inform users of the resources available will be via a web site. We have acquired the URL ‘www.bract.org’ The new web site will be updated and extended to include new vector sets and details of the transformation capability for the different crops. In developing and delivering a transformation capability for the UK research community, we propose to consult widely with potential users to assess future requirements. Through the involvement of a user group, resources will be tailored to meet demand as far as is practicable. It is envisaged that after three years it will be possible to maintain a transformation resource centre for the benefit of the research community by users building full costs for their transformation needs into grant proposals.

  Cytogenetic and molecular characterisation of transgene integration sites using fluorescence in situ hybridisation in wheat. Monsanto Case Award

Many factors influence the stability and expression of transgenes in plants. One factor that cannot be controlled and which has an influence on transgene behaviour is the physical location of the transgene in the genome. This ‘position effect’ may be mediated by a number of factors including chromatin structure and organisation. Very few cytogenetic studies examining the relationship between transgene location and stability have been carried out, and only one has included wheat transformants.
The studentship is based on collaboration between IACR, the University of Cambridge and Monsanto. The principal aim is to locate integration sites and characterise populations of wheat transformants cytogenetically using flouorescence in situ hybridisation (FISH). The genomic location of several different types of constructs in different genetic backgrounds will be determined along with the application of traditional and novel chromosome banding techniques, thus allowing a comparison of insertion sites with chromatin organisation. Many of the transgenic lines have already been analysed for stability of transgene expression and inheritance, thus cytogenetic characterisation of their genomic locations will improve our understanding of the relationship between integration sites and transgene behaviour. Wheat is an ideal species in which to conduct a detailed study because of its commercial importance and well-characterised genetics. The application of such a study in a hexaploid organism may lead to novel data on interactions within and between homeologous genomes.

  Development and application of cereal transformation technologies

Rothamsted Research occupies a leading position world-wide in the ability routinely to genetically transform commercial varieties of wheat. This project aims to increase our understanding of the processes involved and improve the practical utility of wheat transformation. The project also provides 'service' collaborations for scientists within Rothamsted Research to utilise wheat transgenic technologies in the study of agronomic and end-use quality traits of strategic interest.

The genetic transformation of wheat is a technically challenging process confined to a relatively few public-sector laboratories world-wide. During the last decade, we have systematically determined and optimised the variables of DNA delivery and tissue culture that are critical in the wheat transformation process via both biolistics and Agrobacterium. Recent developments have further improved our in-house protocols for transformation via bombardment (eg Pastori et al 2001), and we are refining novel methods for Agrobacterium-mediated DNA transfer to wheat (Amoah et al 2001, Wu et al 2003). As the methods for basic genetic transformation have become increasingly robust, our attention has turned to the mechanisms that control transgene insertion, and also to the isolation, characterisation and utilisation of promoter sequences that deliver transgene expression in particular wheat tissues or at defined stages of development. In this respect we are developing platform technologies for the generation of tagged and knockout wheat lines that will be used to identify genes and promoter sequences and form part of a wider wheat functional genomics initiative within Rothamsted Reseach. There are strong links with other projects, including: 4374, 4149, 4339, 4123, 4038.

  Expression patterns of gluten genes in transgenic wheat and their effect on grain processing properties

Details should only be considered in the context of PU information. To improve the processing quality of bread wheat by transformation with HMW subunit genes. To determine the stability and inheritance of the inserted transgenes.
The objectives are to transfer, using gene-only transformation methods, additional HMW subunit genes to general purpose wheats suitable for UK agriculture.

  RCUK Summer School: Advanced wheat breeding

This is a travel award to attend the RCUK Summer School which will be run jointly with the Chinese Acadamy of Agricultural Sciences and will be held in Beijing during April 2009. The title of Summer School event is: Molecular Technologies for Advanced Wheat breeding: Genetic Transformation and TILLING.

  Tissue culture and transformation of diploid wheat species

Efficient wheat transformation systems, incorporating clean-gene technologies and strong tissue-specific or developmentally regulated promoter sequences will provide novel germplasm for conventional cereal breeding programmes and is also a platform technology with which to study gene function and to identify novel wheat genes via tagging. Gene functional analysis is more flexible in diploid wheat than hexaploid wheat. For this reason, and in order to exploit potentially valuable genes located in diploid wheat genotypes we aim to develop tissue culture and DNA delivery protocols for diploid wheat species, focussing on the likely progenitors of current commercial wheat.

  Use of maize and rice Mar sequences to stabilise the expression of transgenes in wheat

The main objective of this project is to develop and improve GM technology in wheat by exploiting matrix attachment regions (MAR) sequences from the cereals maize and rice. The MARs will be used to stabilize transgene expression using a high molecular weight (HMW) glutenin subunit transgene as a marker. A novel approach will be taken in which the Ac/Ds transposable element system is used to generate new insertion sites after the primary transformation process, to ensure that lines with equal insertion numbers can be compared for MAR function and that the transgene loci examined are not biased by subjection to selection pressure.
Transgenic wheat populations will be produced which contain i) a pair of MARs isolated from rice flanking the wheat high molecular weight glutenin subunit promoter and gene (HMW-1Dx5) ii) a pair of MARs isolated from maize flanking the same cassette iii) a pair of control or ‘mock’ MARs flanking the HMW-1Dx5 promoter and gene. These MAR-flanked expression cassettes will in turn be flanked by Dissociator (Ds) transposable elements from the maize Ac/Ds transposon system. When crossed with wheat lines transformed with the maize Activator (Ac) transposable element, the Ds-flanked expression cassettes will ‘jump’ to novel locations which are free from selection pressure. Since Ac/Ds does not replicate sequences prior to transposition, the transgene copy number in each crossed population should remain constant. Levels of expression from the MAR-flanked HMW glutenin cassettes will be determined and compared for MAR-mediated effects on expression.

  Wheat biotechnology and gene validation platform

Efficient transformation systems are required as platform technologies to facilitate the study of gene function in cereals. This work will provide optimised transformation procedures for wheat and Brachypodium distachyon It will also give insight into localisation of transgene products afforded by incorporating immuno-reactive tags and targeting leader sequences, together with new information on tissue-specific, inducible or developmentally regulated promoter sequences. This will enable more targeted research in relation to wheat grain development and composition as well as nutrient mobilisation and plant/pathogen interactions.

We will set up service-level-agreements to cost and supply the transformation component for inhouse research projects and formal contracts for external collaborations.

Member

  Exploitation of natural plant biodiversity for the presticide-free production of food (BIOEXPLOIT)

The overall objective of this sub-project is to improve the diversity of race-non-specific resistance mechanisms available in hexaploid wheat to combat Fusarium ear blight (FEB) disease. Seven specific objectives will be pursued
1. To explore the cell biology of resistance mechanisms to FEB in newly identified resistance wheat accessions. This will use the already available Fusarium GUS and GFP reporter strains. This study will determine the exact point of hyphal arrest.
2. To quantify Fusarium biomass in infected grain and relate this to the level and profile of deoxynivalenol (DON) mycotoxins.
3. To identify which resistance genotypes confer a DON-mediated resistance phenotype. This will be achieved by parallel inoculations with DON+ and DON- strains.
4. To determine whether any of the novel resistance sources selected for this project contain any of the previously identified QTLs for resistance. This will involve SSR marker analysis.
5. To use a range of promoter TRI: GUS or promoter TRI: GFP transgenic reporter Fusarium strains to determine the onset of DON mycotoxin production in the tissues of novel resistance sources.
6. to explore resistance mechanism synergy. This will be achieved by examining the reactions of F1 plants generated by sexually crossing two different resistance genotypes that appear to operate via distinct mechanisms / have different QTLs /or no known QTLs
7. to determine the field reaction under UK conditions of a new set of resistant Chinese germplasm (non-Sumai-3 based).

Includes studentship.

  Exploiting bioactivity of European cereal grains for improved nutrition and health benefits - HEALTHGRAIN

To develop nutritionally enhanced wheat using a combination of improved varieties and innovative processing, with a focus on dietary fibre, resistant starch and phytochemicals. Should have a range of health benefits for consumers including reduced obesity and type 2 diabetes and improved bowel function. May also result in lower cardiovascular disease and reduced incidents of some forms of cancer.

Includes studentship.

  Exploiting wheat genetic diversity for improved diet and health

This is a China Partnering Award with the Chinese Academy of Agricultural Sciences, Beijing. The aim is to promote collaboration and exchanges of material via short visits and workshops. The focus is on improving the nutritional quality of wheat for humans, including starch composition, soluble fibre content and the content of phytochemicals, vitamins and minerals.

  Extension of systems of genetic improvement

The Meat and Livestock Commission is concerned with producing predictions of genetic merit
In sheep and beef cattle.By use of advanced statistical techniques we will improve estimation
procedures in several areas.These include
Genetic aspects of maternal performance to sustainthe offspring.
The use of crossbred information in combination with pure-bred infornmation inbeef cattle.
The genetics of calving ease and its relevance to the dairy industry.
The development of inernational comparisons.
This will involve using the development of new statistical techniques to measure the influence of different types of collateral and non-collateral relatives.

  The relationship between patterns of gluten protein synthesis and deposition and grain processing quality: a transgenic approach

It is well established that the gluten proteins of wheat are the major determinants of the visco-elastic properties that allow doughs to be processed into bread, pasta and many other food products. Although it is usual to mill the whole starchy endosperm of the grain to produce white flour, this tissue is not homogenous, with evidence for gradients in protein amount and type between the sub-aleurone and peripheral cell layers and the central starchy endosperm cells. Furthermore, there is evidence that two pathways of protein deposition operate, although there is little known about how the operation of these relate to different proteins and cell types. We consider that it is crucial to understand the precise patterns of gluten protein synthesis and deposition as these will determine the structures, composition and properties of the glutenin polymers and hence the end use quality.
The objectives of the research are therefore: -
1. to determine the distribution of the major types of gluten protein within the endosperm of wheat and their presence in vacuolar and/or ER-derived protein bodies in the different cell types, using monoclonal antibodies, epitope tagging and grain fractionation.
2. to manipulate the gluten protein compositions of the sub-aleurone and central parts of the starchy endosperm by using endogenous HMW and LMW subunit promoters to drive transgene expression.
3. to determine the relationship between the composition and deposition pathway of gluten proteins and grain functional properties by analysis of flour fractions derived from the sub-aleurone and starchy endosperm of control and transgenic lines.

  The role of gibberellin in the regulation of wheat grain development

He project investigates the processes regulated by gibberellins (GAs) in cereal grain development, using wheat as the experimental system. The results will indicate whether GAs play a role in determining grain yield and quality and may identify target genes for breeding for these characteristics. Existing transgenic lines with altered GA metabolism in the endosperm will be analysed for effects of transgene expression on grain morphology and in their propensity for preharvest sprouting and pre-maturity alpha-amylase production, both factors which are potentially under GA regulation and which reduce flour quality. Rht dwarfing genes, which confer insensitivity to GA, will be introgressed into the GA-overproducing lines to confirm that a functional GA-signal transduction pathway is necessary for any phenotypic changes identified. Sites of expression within the developing grain of genes of GA biosynthesis and signal transduction will be determined using reporter genes, in situ hybridisation and laser capture microdissection coupled with real time RT-PCR.
The major objectives are to:
- Characterise in detail the morphological, biochemical and molecular phenotypes of wheat grains with altered GA levels
- Identify the target tissues for GA action in grain development through identification of the sites of expression of GA-biosynthetic genes and the use of rht dwarfing genes to modify GA responses in the grain.
- Determine the roles of specific GA-biosynthetic genes in grain development through the isolation of mutants in diploid wheat (Triticum monococcum).
- Test alternative promoters for expression of GA 20-oxidase genes early in endosperm development to affect grain,development without compromising grain quality.

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Publications in Refereed Scientific Journals

L Xia, Y Ma, Yi He and HD Jones (2012) GM wheat development in China: current status and challenges to commercialization. J. Exp. Bot. On-line 15th Dec 2011 doi: 10.1093/jxb/err342

Xiudao Yu, Huw D. Jones, Youzhi Ma, Genping Wang, Zhaoshi Xu, Baoming Zhang, Yongjun Zhang, Guangwei Ren, John A. Pickett & Lanqin Xia (2011) (E)-β-Farnesene synthase genes affect aphid (Myzus persicae) infestation in tobacco (Nicotiana tabacum). Functional & Integrative Genomics On Line DOI 10.1007/s10142-011-0244-1

YW Sun, HD. Jones, Y Yang, S Dreisigacker, SM Li XM Chen, PR. Shewry and LQ. Xia (2011) Haplotype analysis of Viviparous-1 gene in CIMMYT elite bread wheat germplasm (2011) Haplotype analysis of Viviparous-1 gene in CIMMYT elite bread wheat germplasm. Euphytica (On Line Access DOI 10.1007/s10681-011-0482-2

J. Freeman, C.A. Sparks, J. West, P. R. Shewry & H.D. Jones (2011). Temporal and spatial control of transgene expression using a heat-inducible promoter in transgenic wheat. Plant Biotechnology Journal  9: 788–796

C Nemeth, J Freeman, HD Jones, C Sparks, TK Pellny, D Wilkinson, J Dunwell, AAM Andersson, P Åman, F Guillon, L Saulnier, RAC Mitchell and PR Shewry (2010). Down-regulation of the CSLF6 gene results in decreased (1,3;1,4)-β-D-glucan in endosperm of wheat. Plant Physiology. 152: 1209-1218.

F Sestili, M Janni, A Doherty, E Botticella, R D’Ovidio, S Masci, HD Jones, D Lafiandra (2010).  Increasing the amylose content of durum wheat through silencing of the SBEIIa genes. BMC Plant Biology, 10: 144.

Y. He, HD. Jones, S. Chen, X.M. Chen, DW. Wang, KX. Li, DS. Wang and LQ. Xia (2010). Agrobacterium-mediated transformation of durum wheat (Triticum turgidum L. var. durum cv Stewart) with improved efficiency. Journal Experimental Botany, 61: 1567 - 1581.

Xia L. Q., Yang Y., Ma Y. Z., Chen X. M., He Z. H., Roder M. S., Jones H. D. and Shewry P. R. (2009). What can the Viviparous-1 gene tell us about wheat pre-harvest sprouting? Euphytica 168: 385–394.

LF Primavesi, H Wu, EA Mudd, A Day and HD Jones  (2008) Visualisation of plastids in endosperm, pollen and roots of transgenic wheat expressing modified GFP fused to transit peptides from wheat SSU RubisCO, rice FtsZ and maize ferredoxin III proteins. Transgenic Research 17(4): 529-543.

Van Herpen, T., Riley, M., Sparks, C., Jones, H.D., Gritsch, C., Dekking, E.H., Hamer, R.J., Bosch, D., Salentijn, E.M.J., Smulders, M.J.M., Shewry, P.R. and Gilissen, L. (2008) Detailed analysis of the expression of an alpha-gliadin promoter and the deposition of alpha-gliadin protein during wheat grain development. Annals of Botany, 102, 331-342.

H Wu, A Doherty and H D Jones (2008). Efficient and rapid Agrobacterium-mediated genetic transformation of durum wheat (Triticum turgidum L. var. durum) using additional virulence genes. Transgenic Research 17(3): 425-436.

M. Rakszegi, G. Pastori, H.D. Jones, F. Békés, B. Butow, L. Láng, Z. BedÅ‘ and P.R. Shewry (2008). Technological quality of field grown transgenic lines of commercial wheat cultivars expressing the 1Ax1 HMW glutenin subunit gene. Journal of Cereal Science. 47(2): 310-321.

Appleford NEJ, Wilkinson MD, Ma Q, Evans DJ, Stone MC, Pearce SP, Powers SJ, Thomas SG, Jones HD, Phillips AL, Hedden P and Lenton JR (2007) Decreased shoot stature and grain α-amylase activity following ectopic expression of a GA2-oxidase gene in transgenic wheat. Journal of Experimental Botany 58(12): 3213-3226.

GM Pastori, A Huttly, J West, C Sparks, A Pieters, CM. Luna, HD. Jones and CH. Foyer (2007). The maize Ac/Ds system is functional in hexaploid wheat through successive generations. Functional Plant Biology 34: 835-843.

Y Yang, YZ Ma, ZS Xu,  XM Chen, ZH He, Z Yu, M Roder, M Wilkinson, HD Jones, PR Shewry and LQ Xia (2007). Isolation and Expression of Novel Viviparous-1 Genes in Common Wheat (Triticum aestivum L.).  Journal of Experimental Botany 58(11): 2863-2871.

PR Wiley, P Tosi, A Evrard, A Lovegrove, HD Jones and PR Shewry (2007). Promoter Analysis and Immunolocalisation Show That Puroindoline Genes are Exclusively Expressed in Starchy Endosperm Cells of Wheat Grain. Plant Molecular Biology, 64: 125-136.

H Wu, CA Sparks & HD Jones (2006). Characterisation of T-DNA loci and vector backbone sequences in transgenic wheat produced by Agrobacterium-mediated transformation. Molecular Breeding, 18: 195-208. 

Peter R Shewry, Stephen Powers, J Michael Field, Roger J Fido, Huw D Jones, Gillian M Arnold, Jevon West, Paul A Lazzeri, Pilar Barcelo, Francisco Barro, Arthur S Tatham, Frank Bekes, Barbara Butow and Helen Darlington (2006). Comparative Field Performance over Three Years and Two Sites of Transgenic Wheat Lines Expressing HMW Subunit Transgene. Theor. Appl. Genet. 113: 128-136.

Huw D Jones, Angela Doherty and Huixia Wu (2005). Review of Methodologies and a Protocol for the Agrobacterium-mediated transformation of wheat. Plant Methods 1: 5.

Shewry PR and Jones HD. (2005). Transgenic wheat: where do we stand after the first 12 years? Annals of Applied Biology 147 (1), 1-14.

Guang Y He; Huw D Jones; Renato D'Ovidio; Stefania Masci; Mingjie Chen; Jevon West; Barbara Butow; Olin D Anderson; Paul Lazzeri; Roger Fido; Peter Shewry Expression of an extended HMW subunit in transgenic wheat and the effect on dough mixing properties (2005).  Journal of Cereal Science. 42: 225-231.

Jonathan R. Howarth, Jean N. Jacquet, Angela Doherty, Huw D. Jones  and Martin E. Cannell (2005). Molecular genetic analysis of silencing in two lines of Triticum aestivum transformed with pAHC25. Annals of Applied Biology 146: 311-320.

Jones Huw D (2005). Wheat transformation: Current technology and applications to grain development and composition. Journal of Cereal Science. Vol 41 (2): 137-147.

Chrimes D, Rogers HJ, Frances D, Jones HD and Ainsworth C (2005). Expression of fission yeast cdc25 driven by the wheat ADP-glucose pyrophosphorylase large subunit promoter perturbs both pollen and ovule development in transgenic wheat. NewPhytologist, Vol 166 (1): 185-192.

N-N Shi, He G-Y, Li KX, Jones HD & Shewry PR (2005). Transformation and Expression of High Molecular Weight Glutenin Subunit Genes in Common Wheat (Triticum aestivum L.) varieties. Scientia Agricultura Sinica 38(5): 874-881.

Mendoza de Gyves E, Sparks C, Sayanova O, Lazzeri P, Napier J A, and Jones, H D (2004). Genetic manipulation of g-linolenic acid (GLA) synthesis in commercial varieties of evening primrose (Oenothera Sp.). Plant Biotechnology Journal, 2: 351-357.

Jones HD and Kanyuka K (2004). Can biotechnology and genomics offer better routes to crop protection? Outlooks on Pest Management 15(5): 217-221.

Huixia Wu, Caroline Sparks, Ben Amoah and Huw D Jones (2003). Factors influencing successful Agrobacterium-mediated genetic transformation of wheat. Plant Cell Reports 21: 659-668.

Helen Darlington, Roger Fido, Arthur S. Tatham, Huw Jones, Susan E. Salmon and Peter R. Shewry (2003).  Milling and baking properties of field grown wheat expressing HMW subunit transgenes, Journal of Cereal Science, 38(3): 301-306.

Zhang Y, Darlington H, Jones HD, Halford NG, Napier JA, Lazzeri PA, Shewry PR (2003). Expression of the gamma zein protein of maize in seeds of transgenic barley: effects on grain composition and properties. Theoretical and Applied Genetics 106(6):  1139 – 1146.

Salgueiro S, Matthes M, Gil J, Steele S, Savazzini F, Riley A, Jones HD, Lazzeri PA and Barcelo P (2002). Insertional tagging of regulatory sequences in Tritordeum; a hexaploid cereal species. Theoretical & Applied Genetics 104 : 916-925.

P.R Shewry A.S Tatham R. Fido H. Jones  P. Barcelo  P.A. Lazzeri (2001). Improving the end use properties of wheat by manipulating the grain protein composition.  Euphytica  119 (1/2): 45-48.

Zhang Y, Shewry PR, Jones HD, Barcelo P, Lazzeri PA, Halford NG. (2001) Sugar sensing, metabolic regulation and development: Expression of antisense SnRK1 protein Kinase sequence causes abnormal pollen and embryo sac development in transgenic barley. Plant Journal.28(4): 431-441.

Pastori G, Wilkinson M, Steele S, Sparks C, Jones HD and Parry MAJ (2001). Transformation of elite wheat cultivars at high frequencies. J. Exp. Bot. 52(357): 857-863.

Amoah BK, Wu H, Sparks C, Jones HD (2001).  Factors influencing Agrobacterium-mediated transient expression of uidA in wheat inflorescence tissue. J. Exp. Bot. 52(358): 1135-1142.

E. Mendoza de Gyves, C.A. Sparks, A.F. Fieldsend, P.A. Lazzeri, and H.D. Jones (2001). High frequency of adventitious shoot regeneration from commercial cultivars of evening primrose (Oenothera spp.) using thidiazuron. Annals of Applied Biology 138: 329-332.

Huw D Jones, Smita Kurup, Nicholas CB Peters and Michael J Holdsworth (2000). Identification and analysis of proteins that interact with the Avena fatua homologue of the maize transcription factor VIVIPAROUS 1.  Plant Journal 21(2) 133-142.

Smita Kurup, Huw D Jones and Michael J Holdsworth (2000). Interactions of the developmental regulator ABI3 with proteins identified from developing Arabidopsis seeds. Plant Journal 21(2) 143-157.

Duncan A.N. Edlin, Peter Kille, Mark D. Wilkinson, Huw D Jones and John L. Harwood (2000) Morphological and Metabolic Changes in Transgenic Wheat with Altered Glycerol 3-Phosphate Acyltransferase or Acyl-ACP Thioesterase Activities. Biochemical Society Transactions 28: 682-683.

HD Jones, S Smith, R Desikan, S Plakidou-Dymock, A Lovegrove and R Hooley (1998). Heterotrimeric G proteins may be involved in gibberellin-induction of α-amylase gene expression in aleurone.  Plant Cell 10(2): 1-10.

HD Jones, MJ Holdsworth & R Hooley (1997). A full length cDNA, af10, isolated from Avena fatua aleurone encodes a protein with similarity to clathrin assembly proteins. Plant Physiology 113, 663.

HD Jones, NCB Peters and M Holdsworth (1997). Genotype and environment interact to control dormancy and differential expression of the Viviparous 1 homologue in embryos of Avena fatua. Plant Journal 12(4): 911-921.

Books and Book Chapters

Jones, HD., Gebruers, K., Ward, J, and Shewry, PR. 2010. HealthGrain: Increasing the health benefits of wheat. Pages XXX-XXX in: Proc. 4th International Wheat Quality Conference, Chibbar, R. N., and Dexter, J. E. (eds.), AGROBIOS (International), Jodhpur, India.

Jones HD, Sparks CA & Shewry PR (2009) Transgenic manipulation of wheat quality. In: Wheat; Chemistry and Technology 4th Ed. Eds. K Khan & PR Shewry. AACC International MN USA.

Blechl AE and Jones HD (2009) Transgenic Applications in Wheat Improvement. In Wheat: Science and Trade, Ed. Brett F. Carver, Blackwell Publishing.

HD Jones and PR Shewry (2009) Editors and authors, Methods in Biotechnology. Transgenic Wheat, Barley and Oats: Production and Characterisation. Series Ed. J Walker. Humana Press Totowa NJ.

Jones Huw D and Sparks CA (2009) Stable Transformation of Plants. In: Plant Genomics, Methods in Molecular Biology. Eds D Somers, P Langridge and JP Gustafson. Humana Press Totowa, NJ.

Jones Huw D and Sparks CA (2009) Transient Transformation of Plants. In: Plant Genomics, Methods in Molecular Biology. Eds D Somers, P Langridge and JP Gustafson. Humana Press Totowa, NJ.

PR Shewry, HD Jones & NG Halford (2008). Plant Biotechnology: Transgenic Crops. In: Advances in Biochemical Engineering and Biotechnology. Vol.111. Stahl U; Donalies UEB; Nevoigt E (Eds.) 2008, XII, 269p. Springer.

Jones HD, Freeman JA and Sparks CA (2008). A Transgenic approach to understanding gene expression in cereals. In: Cereal Science and Technology for Feeding Ten Billion People: Genomics Era and Beyond. (Options méditerranéennes série A. Séminaires Méditerranéens, N° 81) Eds.: Molina Cano J.L., Christou P., Graner A., Hammer K., Jouve N., Keller B., Lasa J.M., Powell W., Royo C., Shewry P., Stanca A.M. EUCARPIA/CIHEAM. ISBN : 2-85352-404-3

Zhang JU, Li K, Yang G, Luo L, Jones HD, Shewry PR, He G. (2007) Combined Expression of Gluten Strength and Grain Hardness Genes by Crossing Transgenic Plants with Elite Wheat Varieties. In Gluten Proteins 2006, Eds. GL Lookhart & PKW Ng. AACC International Inc.

Maruyama-Funatsuki, W., P. Tosi , H. D. Jones , C. Sparks , A. Riley , H. Yamauchi , and P. R. Shewry. "Production of transgenic wheat transformed with low-molecular-weight glutenin genes aiming for the understanding of the dough strength." 111st meeting of the Japanese Society of Breeding, IKUSHUGAKU KENKYU vol. 9(1), p120 (2007) (in Japanese).

Jones HD, Wilkinson M, Doherty A and Wu H (2007) High Throughput Agrobacterium Transformation of Wheat: a Tool for Functional Genomics. In: Wheat Production in Stressed Environments. Proceedings of the 7th International Wheat Conference, 27 November - 2 December 2005, Mar del Plata, Argentina. Series: Developments in Plant Breeding , Vol. 12. Buck, H.T.; Nisi, J.E.; Salomón, N. (Eds.) 2007, XXI, 795 p. Springer Netherlands.

Shewry PR & Jones HD (2007). Developing allergen-free foods by genetic manipulation. In Managing Allergens in Food, Eds. C Mills, H Wichers & K Hoffmann-Sommergruber. CRC Press.  Woodhead publishing Ltd. Cambridge UK.

Jones HD (2006). Advances in Transformation Technologies. In Plant Biotechnology, Current and Future Applications of Genetically Modified Crops. Ed. Nigel Halford. John Wiley & Sons, Ltd. Chichester, UK. ISBN 0470021810.

PR Shewry, HD Jones & NG Halford (2006). Plant Biotechnology; Transgenic Crops. In Advances in Biochemical Engineering/Biotechnology, Special Volume: Food Biotechnology Eds Stahl & Donalies, Springer Press. In Press.

PR Shewry, P Tosi, HD Jones, CA Sparks, C Gritsch, W Funatsuki, K Niwa, A Huttly, J Napier & J Freeman (2006) Using epitope tagging to explore the trafficking, location and functional properties of wheat gluten proteins. In Proceedings 9th Int. Wheat Gluten Workshop, San Francisco, USA. 14th-16th Sept 2006 AACC , p6-8.

Q Yao, L cong, GY He, JL Chang, KX Li, GX Yang, HD Jones & PR Shewry (2006). Simple integration patterns observed by transformation with Glu-D1x5 gene as a linear fragment.  In Proceedings 9th Int. Wheat Gluten Workshop, San Francisco, USA. 14th-16th Sept 2006 AACC , 112-116.

J Goodwin, G Pastori, M Davey & H D Jones (2005).  Selectable Markers: antibiotic and herbicide resistance, pp 191-203. In Transgenic Plants: Methods and Protocols. Ed. L Pena. Humana Press.

Harwood WA, Irwin J, Snape J and Jones HD (2004). Plant transformation facility for the UK research community. Annual Wheat Newsletter 50: 194-195.

Rob Reid, Huw D Jones & Harry Smith (2004). Phytochromes - Biotechnological Prospects, pp 765-738,.  In Handbook of Plant Biotechnology, Eds. Paul Christou and Harry Klee. ISBN: 0-471-85199-X. John Wiley & Sons, Ltd. Chichester, UK.

Caroline Sparks and Huw D Jones (2004). Transformation of Wheat by Biolistics. In; Transgenic Crops of the World. Ed. Ian S Curtis. 454p, ISBN: 1-4020-2332-4. Springer.

Huw D Jones (2004) Book review, Genetically Modified Organisms: Transgenesis in Plants, Y Tourte, (2003). Annals of Botany 93: 115-116.

Huw D Jones (2003). Transformation – General Principles. In Encyclopedia of Applied Plant Science Eds. B. Thomas, D. Murphy and B. Murray. Academic Press.

Huw D Jones & Martin Cannell (2003). Genetics of Crop Improvement - Transgene Stability and Inheritance. In Encyclopedia of Applied Plant Science Eds. B. Thomas, D. Murphy and B. Murray. Academic Press.

Jones HD & Wiley P (2003). The Double Helix- fifty years on. The Biologist 50 (2) 53-57.

M E Cannell and H D Jones (2003) Transgenic Wheat.  In: Plant Genetic Engineering Vol. 2: Improvement Of Food Crops. Eds. Pawan K. Jaiwal And Rana P.Singh. Pub: Sci-Tech.Pub.Co.P.O. Box-720 728, Houston, Texas 77272 U.S.A. ISBN: 1-930813-02-3.

Jones HD, Wilkinson MD (2000).  Transformation of elite wheat varieties for improved end-use qualities: modification of gibberellin levels and pre-harvest sprouting.  In: Wheat in a Global Environment - Proceedings of 6th International Wheat Conference. Developments in plant breeding Vol 9. Kluwer Academic Publishers, ISBN 0-7923-6722-7 Dec 2000.

Pastori GM, Steele SH, Jones HD, Shewry PR. (2000). Transformation of commercial wheat varieties with high molecular weight glutenin subunit genes. . In: Wheat Gluten, Eds. P.R. Shewry & A.S. Tatham. Pub: Royal Society of Chemistry, ISBN 0-85404-865-0.

Peter R Shewry, Arthur S Tatham, Roger Fido, Huw D Jones, Pilar Barcelo & Paul Lazzeri (2000). Improving the End Use Properties of Wheat by manipulation the grain protein composition. In: Advances in Botanical Research, Biotechnology of Cereals, Ed. P Shewry. Academic Press.

HD Jones (1999). Dormancy, a survival mechanism for seeds, or a useful tool for agriculture. The Biologist. 46(2): 65-69.

 Opinions and Outputs of the EFSA GMO Panel published in the EFSA Journal

Hans Christer Andersson, Salvatore Arpaia, Detlef Bartsch, Josep Casacuberta, Howard Davies, Patrick du Jardin, Gerhard Flachowsky, Lieve Herman, Huw Jones, Sirpa Kärenlampi, Jozsef Kiss, Gijs Kleter, Harry Kuiper, Antoine Messéan, Kaare Magne Nielsen, Joe Perry, Annette Pöting, Jeremy Sweet, Christoph Tebbe, Atte Johannes von Wright, and Jean-Michel Wal (2011). Guidance on the risk assessment of genetically modified microorganisms and their products intended for food and feed use EFSA Panel on Genetically Modified Organisms (GMO). EFSA Journal 2011;9(6): 2193 [54 pp.]. doi: 10.2903/j.efsa.2011.2193

Hans Christer Andersson, Salvatore Arpaia, Detlef Bartsch, Josep Casacuberta, Howard Davies, Patrick du Jardin, Gerhard Flachowsky, Lieve Herman, Huw Jones, Sirpa Kärenlampi, Jozsef Kiss, Gijs Kleter, Harry Kuiper, Antoine Messéan, Kaare Magne Nielsen, Joe Perry, Annette Pöting, Jeremy Sweet, Christoph Tebbe, Atte Johannes von Wright, and Jean-Michel Wal (2011). Guidance for risk assessment of food and feed from genetically modified plants EFSA Panel on Genetically Modified Organisms (GMO). EFSA Journal 2011; 9(5): 2150 [37 pp.]. doi: 10.2903/j.efsa.2011.2150

Hans Christer Andersson, Salvatore Arpaia, Detlef Bartsch, Josep Casacuberta, Howard Davies, Patrick du Jardin, Gerhard Flachowsky, Lieve Herman, Huw Jones, Sirpa Kärenlampi, Jozsef Kiss, Gijs Kleter, Harry Kuiper, Antoine Messéan, Kaare Magne Nielsen, Joe Perry, Annette Pöting, Jeremy Sweet, Christoph Tebbe, Atte Johannes von Wright, and Jean-Michel Wal. (2011). Guidance on selection of comparators for the risk assessment of genetically modified plants and derived food and feed EFSA Panel on Genetically Modified Organisms (GMO). EFSA Journal 2011; 9(5): 2149 [21 pp.]. doi: 10.2903/j.efsa.2011.2149

Hans Christer Andersson, Salvatore Arpaia, Detlef Bartsch, Josep Casacuberta, Howard Davies, Patrick du Jardin, Gerhard Flachowsky, Lieve Herman, Huw Jones, Sirpa Kärenlampi, Jozsef Kiss, Gijs Kleter, Harry Kuiper, Antoine Messéan, Kaare Magne Nielsen, Joe Perry, Annette Pöting, Jeremy Sweet, Christoph Tebbe, Atte Johannes von Wright, and Jean-Michel Wal (2011). Scientific Opinion on application (EFSA-GMO-NL-2008-52) for the placing on the market of herbicide tolerant genetically modified soybean A5547-127 for food and feed uses, import and processing under Regulation (EC) No 1829/2003 from Bayer CropScience EFSA Panel on Genetically Modified Organisms (GMO). The EFSA Journal (2011);9(5): 2147 [27 pp.]. doi: 10.2903/j.efsa.2011.2147

Hans Christer Andersson, Salvatore Arpaia, Detlef Bartsch, Josep Casacuberta, Howard Davies, Patrick du Jardin, Gerhard Flachowsky, Lieve Herman, Huw Jones, Sirpa Kärenlampi, Jozsef Kiss, Gijs Kleter, Harry Kuiper, Antoine Messéan, Kaare Magne Nielsen, Joe Perry, Annette Pöting, Jeremy Sweet, Christoph Tebbe, Atte Johannes von Wright, and Jean-Michel Wal (2011). Scientific Opinion on applications (EFSA-GMO-RX-40-3-2[8-1a/20-1a], EFSA-GMO-RX-40-3-2[8-1b/20-1b]) for renewal of authorisation for the continued marketing of (1) food containing, consisting of, or produced from genetically modified soybean 40-3-2; (2) feed containing, consisting of, or produced from soybean 40-3-2; (3) other products containing or consisting of soybean 40-3-2 with the exception of cultivation, all under Regulation (EC) No 1829/2003 from Monsanto EFSA Panel on Genetically Modified Organisms (GMO). EFSA Journal 2010;8(12): 1908 [38 pp.]. doi: 10.2903/j.efsa.2010.1908

Hans Christer Andersson, Salvatore Arpaia, Detlef Bartsch, Josep Casacuberta, Howard Davies, Patrick du Jardin, Gerhard Flachowsky, Lieve Herman, Huw Jones, Sirpa Kärenlampi, Jozsef Kiss, Gijs Kleter, Harry Kuiper, Antoine Messéan, Kaare Magne Nielsen, Joe Perry, Annette Pöting, Jeremy Sweet, Christoph Tebbe, Atte Johannes von Wright, and Jean-Michel Wal (2010). Scientific Opinion on application (EFSA-GMO-CZ-2008-62) for the placing on the market of insect resistant and herbicide tolerant genetically modified maize MON 89034 x 1507 x MON 88017 x 59122 and all sub-combinations of the individual events as present in its segregating progeny, for food and feed uses, import and processing under Regulation (EC) No 1829/2003 from Dow AgroSciences and Monsanto EFSA Panel on Genetically Modified Organisms (GMO).. EFSA Journal 2010; 8(9): 1781 [37 pp.]. doi: 10.2903/j.efsa.2010.1781

Hans Christer Andersson, Salvatore Arpaia, Detlef Bartsch, Josep Casacuberta, Howard Davies, Patrick du Jardin, Gerhard Flachowsky, Lieve Herman, Huw Jones, Sirpa Kärenlampi, Jozsef Kiss, Gijs Kleter, Harry Kuiper, Antoine Messéan, Kaare Magne Nielsen, Joe Perry, Annette Pöting, Jeremy Sweet, Christoph Tebbe, Atte Johannes von Wright, and Jean-Michel Wal (2010). Guidance on the environmental risk assessment of genetically modified plants EFSA Panel on Genetically Modified Organisms (GMO). EFSA Journal 2010;8(11): 1879 [111 pp.]. doi: 10.2903/j.efsa.2010.1879

Hans Christer Andersson, Salvatore Arpaia, Detlef Bartsch, Josep Casacuberta, Howard Davies, Patrick du Jardin, Gerhard Flachowsky, Lieve Herman, Huw Jones, Sirpa Kärenlampi, Jozsef Kiss, Gijs Kleter, Harry Kuiper, Antoine Messéan, Kaare Magne Nielsen, Joe Perry, Annette Poeting, Jeremy Sweet, Christoph Tebbe, Atte Johannes von Wright, and Jean-Michel Wal (2010). Scientific Opinion on the assessment of potential impacts of genetically modified plants on non-target organisms EFSA Panel on Genetically Modified Organisms (GMO). EFSA Journal 2010;8(11): 1877 [72 pp.]. doi: 10.2903/j.efsa.2010.1877

Hans Christer Andersson, Salvatore Arpaia, Detlef Bartsch, Josep Casacuberta, Howard Davies, Patrick du Jardin, Gerhard Flachowsky, Lieve Herman, Huw Jones, Sirpa Kärenlampi, Jozsef Kiss, Gijs Kleter, Harry Kuiper, Antoine Messéan, Kaare Magne Nielsen, Joe Perry, Annette Pöting, Jeremy Sweet, Christoph Tebbe, Atte Johannes von Wright, and Jean-Michel Wal (2010). Scientific Opinion on an application (EFSA-GMO-NL-2009-65) for the placing on the market of insect resistant and herbicide tolerant genetically modified maize MON 89034 × 1507 × NK603 and all sub-combinations of the individual events as present in its segregating progeny, for food and feed uses, import and processing under Regulation (EC) No 1829/2003 from Dow AgroSciences and Monsanto EFSA Panel on Genetically Modified Organisms (GMO). EFSA Journal 2010; 8(9): 1782 [34 pp.]. doi: 10.2903/j.efsa.2010.1782

Hans Christer Andersson, Salvatore Arpaia, Detlef Bartsch, Josep Casacuberta, Howard Davies, Patrick du Jardin, Gerhard Flachowsky, Lieve Herman, Huw Jones, Sirpa Kärenlampi, Jozsef Kiss, Gijs Kleter, Harry Kuiper, Antoine Messéan, Kaare Magne Nielsen, Joe Perry, Annette Pöting, Jeremy Sweet, Christoph Tebbe, Atte Johannes von Wright, and Jean-Michel Wal (2010). Scientific Opinion on the assessment of allergenicity of GM plants and microorganisms and derived food and feed EFSA Panel on Genetically Modified Organisms (GMO). EFSA Journal 2010; 8(7): 1700 [168 pp.]. doi: 10.2903/j.efsa.2010.1700

Hans Christer Andersson, Salvatore Arpaia, Detlef Bartsch, Josep Casacuberta, Howard Davies, Patrick du Jardin, Gerhard Flachowsky, Lieve Herman, Huw Jones, Sirpa Kärenlampi, Jozsef Kiss, Gijs Kleter, Harry Kuiper, Antoine Messéan, Kaare Magne Nielsen, Joe Perry, Annette Pöting, Jeremy Sweet, Christoph Tebbe, Atte Johannes von Wright, and Jean-Michel Wal (2010). Scientific Opinion on application (EFSA-GMO-NL-2005-16) for the placing on the market of insect resistant genetically modified cotton (Gossypium hirsutum L.) 281-24-236 x 3006-210-23 for food and feed uses, import and processing under Regulation (EC) No 1829/2003 from Dow AgroSciences EFSA Panel on Genetically Modified Organisms (GMO). EFSA Journal 2010; 8(6): 1644 [32 pp.]. doi: 10.2903/j.efsa.2010.1644

 Hans Christer Andersson, Salvatore Arpaia, Detlef Bartsch, Josep Casacuberta, Howard Davies, Patrick du Jardin, Gerhard Flachowsky, Lieve Herman, Huw Jones, Sirpa Kärenlampi, Jozsef Kiss, Gijs Kleter, Harry Kuiper, Antoine Messéan, Kaare Magne Nielsen, Joe Perry, Annette Pöting, Jeremy Sweet, Christoph Tebbe, Atte Johannes von Wright, and Jean-Michel Wal (2010). Scientific Opinion on application (Reference EFSA-GMO-UK-2008-56) for the placing on the market of insect resistant and herbicide tolerant genetically modified maize Bt11 x MIR604 x GA21 , for food and feed uses, import and processing under Regulation (EC) No 1829/2003 from Syngenta Seeds EFSA Panel on Genetically Modified Organisms (GMO). EFSA Journal 2010; 8(5): 1616 [30 pp.]. doi: 10.2903/j.efsa.2010.1616

Hans Christer Andersson, Salvatore Arpaia, Detlef Bartsch, Josep Casacuberta, Howard Davies, Patrick du Jardin, Gerhard Flachowsky, Lieve Herman, Huw Jones, Sirpa Kärenlampi, Jozsef Kiss, Gijs Kleter, Harry Kuiper, Antoine Messéan, Kaare Magne Nielsen, Joe Perry, Annette Pöting, Jeremy Sweet, Christoph Tebbe, Atte Johannes von Wright, and Jean-Michel Wal (2010). Scientific Opinion on application (Reference EFSA-GMO-UK-2007-50) for the placing on the market of insect resistant and herbicide tolerant genetically modified maize Bt11xMIR604, for food and feed uses, import and processing under Regulation (EC) No 1829/2003 from Syngenta Seeds EFSA Panel on Genetically Modified Organisms (GMO). EFSA journal 2010; 8(5): 1614 [30 pp.]. doi: 10.2903/j.efsa.2010.1614

Hans Christer Andersson, Salvatore Arpaia, Detlef Bartsch, Josep Casacuberta, Howard Davies, Patrick du Jardin, Gerhard Flachowsky, Lieve Herman, Huw Jones, Sirpa Kärenlampi, Jozsef Kiss, Gijs Kleter, Harry Kuiper, Antoine Messéan, Kaare Magne Nielsen, Joe Perry, Annette Poeting, Jeremy Sweet, Christoph Tebbe, Atte Johannes von Wright, and Jean-Michel Wal (2010). Scientific Opinion on application (EFSA-GMO-UK-2007-48) for the placing on the market of insect resistant and herbicide tolerant genetically modified maize MIR604 x GA21 for food and feed uses, import and processing under Regulation (EC) No 1829/2003 from Syngenta Seeds EFSA Panel on Genetically Modified Organisms (GMO). EFSA Journal 2010; 8(5): 1611 [30 pp.]. doi: 10.2903/j.efsa.2010.1611

Hans Christer Andersson, Salvatore Arpaia, Detlef Bartsch, Josep Casacuberta, Howard Davies, Patrick du Jardin, Gerhard Flachowsky, Lieve Herman, Huw Jones, Sirpa Kärenlampi, Jozsef Kiss, Gijs Kleter, Harry Kuiper, Antoine Messéan, Kaare Magne Nielsen, Joe Perry, Annette Pöting, Jeremy Sweet, Christoph Tebbe, Atte Johannes von Wright, and Jean-Michel Wal (2010). Scientific Opinion on the revised molecular characterisation for Rf3 oilseed rape received from the Competent Authority of Belgium under Article 20(3) of Directive 2001/18/EC EFSA Panel on Genetically Modified Organisms (GMO). EFSA Journal 2010;8(5): 1582 [8 pp.]. doi: 10.2903/j.efsa.2010.1582

Hans Christer Andersson, Salvatore Arpaia, Detlef Bartsch, Josep Casacuberta, Howard Davies, Patrick du Jardin, Gerhard Flachowsky, Lieve Herman, Huw Jones, Sirpa Kärenlampi, Jozsef Kiss, Gijs Kleter, Harry Kuiper, Antoine Messéan, Kaare Magne Nielsen, Joe Perry, Annette Pöting, Jeremy Sweet, Christoph Tebbe, Atte Johannes von Wright, and Jean-Michel Wal (2010). Scientific Opinion on application (EFSA-GMO-NL-2007-39) for the placing on the market of insect resistant and herbicide tolerant genetically modified maize MON89034 x MON88017 for food and feed uses, import and processing under Regulation (EC) No 1829/2003 from Monsanto EFSA Panel on Genetically Modified Organisms (GMO). EFSA Journal 2010; 8(3): 1564 [27 pp.]. doi: 10.2903/j.efsa.2010.1564

Hans Christer Andersson, Salvatore Arpaia, Detlef Bartsch, Josep Casacuberta, Howard Davies, Patrick du Jardin, Gerhard Flachowsky, Lieve Herman, Huw Jones, Sirpa Kärenlampi, Jozsef Kiss, Gijs Kleter, Harry Kuiper, Antoine Messéan, Kaare Magne Nielsen, Joe Perry, Annette Pöting, Jeremy Sweet, Christoph Tebbe, Atte Johannes von Wright, and Jean-Michel Wal (2010). Scientific Opinion on applications (EFSA-GMO-RX-MON863) for renewal of the authorisation for continued marketing of existing feed materials, feed additives and food additives produced from maize MON863, under Regulation (EC) No 1829/2003 from Monsanto EFSA Panel on Genetically Modified Organisms (GMO). EFSA Journal 2010; 8(3): 1562 [15 pp.]. doi: 10.2903/j.efsa.2010.1562

Hans Christer Andersson, Salvatore Arpaia, Detlef Bartsch, Josep Casacuberta, Howard Davies, Patrick du Jardin, Gerhard Flachowsky, Lieve Herman, Huw Jones, Sirpa Kärenlampi, Jozsef Kiss, Gijs Kleter, Harry Kuiper, Antoine Messéan, Kaare Magne Nielsen, Joe Perry, Annette Pöting, Jeremy Sweet, Christoph Tebbe, Atte Johannes von Wright, and Jean-Michel Wal (2010). Scientific Opinion on a question from the European Commission related to the notification by Portugal, pursuant to Article 95 (5) of the EC Treaty, for the prohibition of cultivation of Genetically Modified Plants in the Autonomous Region of Madeira EFSA Panel on Genetically Modified Organisms (GMO). EFSA Journal 2010; 8(2): 1500 [7 pp.]. doi: 10.2903/j.efsa.2010.1500

Gabriele Aquilina, Georges Bories, Paul Brantom, Francesca Caloni, Andrew Chesson, Pier Sandro Cocconcelli, Noël Dierick, Mikolaj Gralak, Jürgen Gropp, Ingrid Halle, Joop de Knecht, Reinhard Kroker, Nils-Gunnar Ilbäck, Lubomir Leng, Sven Lindgren, Anne-Katrine Lundebye Haldorsen, Alberto Mantovani, Miklós Mézes, Derek Renshaw, Maria Saarela, Hans Christer Andersson, Salvatore Arpaia, Detlef Bartsch, Josep Casacuberta, Howard Davies, Patrick du Jardin, Gerhard Flachowsky, Lieve Herman, Huw Jones, Sirpa Kärenlampi, Jozsef Kiss, Gijs Kleter, Harry Kuiper, Antoine Messéan, Kaare Magne Nielsen, Joe Perry, Annette Poeting, Jeremy Sweet, Christoph Tebbe, Atte Johannes von Wright and Jean-Michel Wal (2010). Scientific Opinion on the safety and efficacy of L-isoleucine for all animal species EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP). EFSA Journal 2010; 8(1): 1425 [19 pp.]. doi: 10.2903/j.efsa.2010.1425

Hans Christer Andersson, Salvatore Arpaia, Detlef Bartsch, Josep Casacuberta, Howard Davies, Patrick du Jardin, Gerhard Flachowsky, Lieve Herman, Huw Jones, Sirpa Kärenlampi, Jozsef Kiss, Gijs Kleter, Harry Kuiper,Antoine Messéan, Kaare Magne Nielsen, Joe Perry, Annette Pöting, Jeremy Sweet, Christoph Tebbe, Atte Johannes von Wright, and Jean-Michel Wal (2009). Scientific Opinion on applications (EFSA-GMO-RX-GT73) for renewal of the authorisation for continued marketing of existing (1) food and food ingredients produced from oilseed rape GT73; and of (2) feed materials, feed additives and food additives produced from oilseed rape GT73, all under Regulation (EC) No 1829/2003 from Monsanto EFSA Panel on Genetically Modified Organisms (GMO). EFSA Journal 2009; 7(12): 1417 [12 pp.]. doi: 10.2903/j.efsa.2009.1417

Hans Christer Andersson, Salvatore Arpaia, Detlef Bartsch, Josep Casacuberta, Howard Davies, Patrick du Jardin, Gerhard Flachowsky, Lieve Herman, Huw Jones, Sirpa Kärenlampi, Jozsef Kiss, Gijs Kleter, Harry Kuiper, Antoine Messéan, Kaare Magne Nielsen, Joe Perry, Annette Poeting, Jeremy Sweet, Christoph Tebbe, Atte Johannes von Wright, and Jean-Michel Wal (2009). Statement on a scientific publication on vertical gene flow in rice and its potential ecological consequences by Lu & Yang (2009) EFSA Panel on Genetically Modified Organisms (GMO). EFSA Journal 2009; 7(11): 1365 [9 pp.]. doi: 10.2903/j.efsa.2009.1365

Hans Christer Andersson, Salvatore Arpaia, Detlef Bartsch, Josep Casacuberta, Howard Davies, Patrick du Jardin, Gerhard Flachowsky, Lieve Herman, Huw Jones, Sirpa Kärenlampi, Jozsef Kiss, Gijs Kleter, Harry Kuiper, Antoine Messéan, Kaare Magne Nielsen, Joe Perry, Annette Pöting, Jeremy Sweet, Christoph Tebbe, Atte Johannes von Wright, and Jean-Michel Wal (2009). Scientific Opinion on application (EFSA-GMO-NL-2007-38) for the placing on the market of insect resistant and/or herbicide tolerant genetically modified maize MON89034 x NK603 for food and feed uses, import and processing under Regulation (EC) No 1829/2003 from Monsanto EFSA Panel on Genetically Modified Organisms (GMO). EFSA Journal 2009; 7(9): 1320 [29 pp.]. doi: 10.2903/j.efsa.2009.1320

Hans Christer Andersson, Salvatore Arpaia, Detlef Bartsch, Josep Casacuberta, Howard Davies, Patrick du Jardin, Gerhard Flachowsky, Lieve Herman, Huw Jones, Sirpa Kärenlampi, Jozsef Kiss, Gijs Kleter, Harry Kuiper, Antoine Messéan, Kaare Magne Nielsen, Joe Perry, Annette Poeting, Jeremy Sweet, Christoph Tebbe, Atte Johannes von Wright, and Jean-Michel Wal (2009). Scientific Opinion on application (EFSA-GMO-UK-2007-49) for the placing on the market of the insect resistant and herbicide tolerant genetically modified maize Bt11xGA21 for food and feed uses, import and processing under Regulation (EC) No 1829/2003 from Syngenta Seeds EFSA Panel on Genetically Modified Organisms (GMO). EFSA Journal 2009; 7(9): 1319 [27 pp.]. doi: 10.2903/j.efsa.2009.1319

Hans Christer Andersson, Salvatore Arpaia, Detlef Bartsch, Josep Casacuberta, Howard Davies, Patrick du Jardin, Gerhard Flachowsky, Lieve Herman, Huw Jones, Sirpa Kärenlampi, Jozsef Kiss, Gijs Kleter, Harry Kuiper, Antoine Messéan, Kaare Magne Nielsen, Joe Perry, Annette Pöting, Jeremy Sweet, Christoph Tebbe, Atte Johannes von Wright, and Jean-Michel Wal (2009). Application (EFSA-GMO-RX-MS8-RF3) for renewal of the authorisation for continued marketing of existing (1) food and food ingredients produced from genetically modified glufosinate-tolerant oilseed rape Ms8, Rf3 and Ms8 x Rf3, and (2) feed materials produced from genetically modified glufosinate-tolerant oilseed rape Ms8, Rf3 and Ms8 x Rf3, under Regulation (EC) No 1829/2003 from Bayer CropScience EFSA Panel on Genetically Modified Organisms (GMO). EFSA Journal 2009; 7(9): 1318 [12 pp.]. doi: 10.2903/j.efsa.2009.1318

Gabriele Aquilina, Georges Bories, Paul Brantom, Francesca Caloni, Andrew Chesson, Pier Sandro Cocconcelli, Joop de Knecht, Noël Albert Dierick, Mikolaj Antoni Gralak, Jürgen Gropp, Ingrid Halle, Nils-Gunnar Ilbäck, Reinhard Kroker, Peadar Lawlor, Lubomir Leng, Sven Lindgren, Anne-Katrine Lundebye Haldorsen, Alberto Mantovani, Miklós Mézes, Derek Renshaw, Maria Saarela, Hans Christer Andersson, Salvatore Arpaia, Detlef Bartsch, Josep Casacuberta, Howard Davies, Gerhard Flachowsky, Patrick du Jardin, Huw Jones, Lieve Herman, Sirpa Kärenlampi, Jozsef Kiss, Gijs Kleter, Harry A. Kuiper, Antoine Messéan, Kaare Nielsen, Joe Perry, Annette Pöting, Jeremy B. Sweet, Christoph Tebbe, Atte von Wright and Jean-Michel Wal (2009). Safety and efficacy of Ronozyme® ProAct (serine protease) for use as feed additive for chickens for fattening EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP). doi: 10.2903/j.efsa.2009.1185

Hans Christer Andersson, Salvatore Arpaia, Detlef Bartsch, Josep Casacuberta, Howard Davies, Patrick du Jardin, Gerhard Flachowsky, Lieve Herman, Huw Jones, Sirpa Kärenlampi, Jozsef Kiss, Gijs Kleter, Harry Kuiper, Antoine Messéan, Kaare Magne Nielsen, Joe Perry, Annette Pöting, Jeremy Sweet, Christoph Tebbe, Atte Johannes von Wright, and Jean-Michel Wal (2009). Application (Reference EFSA-GMO-CZ-2006-33) for the placing on the market of the insect-resistant and glyphosate-tolerant genetically modified maize MON 88017 x MON 810, for food and feed uses, import and processing under Regulation (EC) No 1829/2003 from Monsanto EFSA Panel on Genetically Modified Organisms (GMO).. doi: 10.2903/j.efsa.2009.1192

Hans Christer Andersson, Salvatore Arpaia, Detlef Bartsch, Josep Casacuberta, Howard Davies, Patrick du Jardin, Gerhard Flachowsky, Huw Jones, Lieve Herman, Sirpa Kärenlampi, Jozsef Kiss, Gijs Kleter, Harry Kuiper, Antoine Messéan, Kaare Magne Nielsen, Joe Perry, Annette Pöting, Jeremy Sweet, Christoph Tebbe, Atte Johannes von Wright and Jean-Michel Wal (2009). Application (Reference EFSA-GMO-UK-2005-11) for the placing on the market of insect-resistant genetically modified maize MIR604 event, for food and feed uses, import and processing under Regulation (EC) No 1829/2003 from Syngenta Seeds S.A.S on behalf of Syngenta Crop Protection AG EFSA Panel on Genetically Modified Organisms (GMO). doi: 10.2903/j.efsa.2009.1193

Hans Christer Andersson, Salvatore Arpaia, Detlef Bartsch, Josep Casacuberta, Howard Davies, Gerhard Flachowsky, Patrick du Jardin, Huw Jones, Lieve Herman, Sirpa Kärenlampi, Jozsef Kiss, Gijs Kleter, Harry A. Kuiper, Antoine Messéan, Kaare Nielsen, Joe Perry, Annette Pöting, Jeremy B. Sweet, Christoph Tebbe, Atte von Wright and Jean-Michel Wal (2009). Opinion on a request from the European Commission related to the enzyme preparation of trade name “Danisco Xylanase G/L (endo-1,4-beta-xylanase)” as a feed additive for laying hens and chickens and ducks for fattening. EFSA Panel on Genetically Modified Organisms (GMO). doi: 10.2903/j.efsa.2009.1183